A5067440472- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Insect-Plant Interactions and Control
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Transgenic Plants and Applications
- Agricultural pest management studies
- Plant Virus Research Studies
- Antimicrobial Peptides and Activities
- Plant Stress Responses and Tolerance
- SARS-CoV-2 and COVID-19 Research
- Insect and Pesticide Research
- Plant Reproductive Biology
- Vibrio bacteria research studies
- Plant and Biological Electrophysiology Studies
- Salmonella and Campylobacter epidemiology
- Animal Virus Infections Studies
- Plant and animal studies
- Genetic and Environmental Crop Studies
- Bioinformatics and Genomic Networks
- Speech Recognition and Synthesis
- Nematode management and characterization studies
- Plant responses to elevated CO2
- Light effects on plants
- Circadian rhythm and melatonin
University of Washington
2019-2025
Washington Research Foundation
2020-2022
Seattle University
2019-2022
University of California, San Diego
2019-2020
University of California, Berkeley
2011-2017
University of Pennsylvania
2015
Tufts University
2011-2012
Summary Xanthomonas spp. are phytopathogenic bacteria that can cause disease on a wide variety of plant species resulting in significant impacts crop yields. Limited genetic resistance is available most and current control methods often inadequate, particularly when environmental conditions favor disease. The Nicotiana benthamiana has been shown to be resistant Pseudomonas due an immune response triggered by the bacterial effector proteins XopQ HopQ1, respectively. We used reverse screen...
Herbivory is fundamental to the regulation of both global food webs and extent agricultural crop losses. Induced plant responses herbivores promote resistance often involve perception specific herbivore-associated molecular patterns (HAMPs); however, precisely defined receptors elicitors associated with herbivore recognition remain elusive. Here, we show that a receptor confers signaling defense outputs in response HAMP common caterpillar oral secretions (OS). Staple crops, including cowpea...
Plants and animals detect biomolecules termed microbe-associated molecular patterns (MAMPs) induce immunity. Agricultural production is severely impacted by pathogens which can be controlled transferring immune receptors. However, most studies use a single MAMP epitope the impact of diverse multicopy MAMPs on induction unknown. Here, we characterized landscape from five proteinaceous across 4,228 plant-associated bacterial genomes. Despite diversity sampled, natural variation was constrained...
The in planta association of the Hyaloperonospora arabidopsidis effector ATR1 with cognate Arabidopsis thaliana RPP1 immune receptor activates a disease-resistance signaling pathway that inhibits pathogen growth. To define molecular events specifying recognition by RPP1, we determined crystal structure and assayed effects surface polymorphisms are critical to activating plant immunity. adopts an elongated, all-helical, two-domain, seahorse-like overall architecture unlike any previously...
In plants, specific recognition of pathogen effector proteins by nucleotide-binding leucine-rich repeat (NLR) receptors leads to activation immune responses. RPP1, an NLR from Arabidopsis thaliana, recognizes the ATR1, oomycete Hyaloperonospora arabidopsidis, direct association via C-terminal repeats (LRRs). Two RPP1 alleles, RPP1-NdA and RPP1-WsB, have narrow broad spectra, respectively, with recognizing a subset ATR1 variants recognized RPP1-WsB. this work, we further characterized through...
Abstract Upon herbivory, plants can swiftly reallocate newly acquired resources to different tissues within a plant. Although the herbivore‐induced movement of is apparent, direction and role remobilized are not well understood. Here, we used two‐pronged approach combining radioisotope metabolomic techniques shed light on whole‐plant resource reallocation changes in primary metabolism tomato, Solanum lycopersicum (L.) (Solanaceae), model response simulated herbivory by specialist Manduca...
ABSTRACT Mouse hepatitis virus (MHV) isolates JHM.WU and JHM.SD promote severe central nervous system disease. However, while replicates robustly induces hepatitis, fails to replicate or induce pathology in the liver. These two JHM variants encode homologous proteins with few polymorphisms, little is known about which viral proteins(s) responsible for liver tropism of JHM.WU. We constructed reverse genetic systems and, utilizing these full-length cDNA clones, chimeric viruses mapped...
As a first step in innate immunity, pattern recognition receptors (PRRs) recognize the distinct pathogen and herbivore-associated molecular patterns mediate activation of immune responses, but specific steps evolution new PRR sensing functions are not well understood. We employed comparative genomic functional analyses to define evolutionary events leading peptide inceptin (In11) by receptor (INR) legume plant species. Existing de novo genome assemblies revealed that presence INR gene...
Summary Nucleotide‐binding leucine‐rich repeat ( NB ‐ LRR , or NLR ) receptors mediate pathogen recognition. The Arabidopsis thaliana RPP 1 recognizes the tandem WY ‐domain effector ATR from oomycete Hyaloperonospora arabidopsidis through direct association with C‐terminal s. We isolated and characterized homologous genes 1‐EstA 1‐ZdrA two ecotypes, Estland (Est‐1) Zdarec (Zdr‐1), responsible for recognizing a novel spectrum of alleles. ‐ZdrA encode nearly identical s that are...
SUMMARY Chewing herbivores activate plant defense responses through a combination of mechanical wounding and elicitation by herbivore‐associated molecular patterns (HAMPs). HAMPs are wound response amplifiers; however, specific outputs may also exist that strictly require HAMP‐mediated signaling. To investigate signaling responses, we characterized cowpea ( Vigna unguiculata ) transcriptome changes following inceptin, peptide HAMP common in Lepidoptera larvae oral secretions. Following...
Crop damage by herbivorous insects remains a significant contributor to annual yield reductions. Following attack, maize (Zea mays) responds herbivore-associated molecular patterns (HAMPs) and damage-associated (DAMPs), activating dynamic direct indirect antiherbivore defense responses. To define underlying signaling processes, comparative analyses between plant elicitor peptide (Pep) DAMPs fatty acid-amino acid conjugate (FAC) HAMPs were conducted. RNA sequencing analysis of early...
Abstract Plant-herbivore interactions are ubiquitous across nature and drive major agricultural losses. Inducible defense responses triggered through immune recognition aid in host plant protection; however, specific ligand-receptor pairs mediating the initial perception of herbivory remain unknown. Plants subtribe Phaseolinae detect herbivore-associated peptides caterpillar oral secretions defined ligands proteolytic fragments chloroplastic ATP synthase termed inceptins. Using forward...
This strategic plan summarizes the major accomplishments achieved in last quinquennial by soybean [Glycine max (L.) Merr.] genetics and genomics research community outlines key priorities for next 5 years (2024-2028). work is result of deliberations among over 50 researchers during a 2-day workshop St Louis, MO, USA, at end 2022. The divided into seven traditional areas/disciplines: Breeding, Biotic Interactions, Physiology Abiotic Stress, Functional Genomics, Biotechnology, Genomic...
Many biological systems across scales of size and complexity exhibit a time-varying complex network structure that emerges self-organizes as result interactions with the environment. Network optimize some intrinsic cost functions are unknown involve for example energy efficiency, robustness, resilience, frailty. A wide range networks exist in biology, from gene regulatory important organismal development, protein interaction govern physiology metabolism, neural store convey information to...
Plants rely on innate immune systems to defend against a wide variety of biotic attackers. Key components immunity include cell-surface pattern-recognition receptors (PRRs), which recognize pest- and pathogen-associated molecular patterns (PAMPs). Unlike other classes that often have visible cell-death outputs upon activation, PRRs generally lack rapid methods for assessing function. Here, we describe genetically encoded bioluminescent reporter activation by heterologously expressed in the...
Abstract Plants and animals detect biomolecules termed Microbe-Associated Molecular Patterns (MAMPs) induce immunity. Agricultural production is severely impacted by pathogens which can be controlled transferring immune receptors. However, most studies use a single MAMP epitope the impact of diverse multi-copy MAMPs on induction unknown. Here we characterized landscape from five proteinaceous across 4,228 plant-associated bacterial genomes. Despite diversity sampled, natural variation was...
Summary Plants activate induced defenses through the recognition of molecular patterns. Like pathogen-associated patterns (PAMPs), herbivore-associated (HAMPs) can be recognized by cell surface pattern receptors leading to defensive transcriptional changes in host plants. Herbivore-induced outputs are regulated circadian clock, but underlying mechanisms remain unknown. To investigate how plant clock regulates reprogramming a specific HAMP-induced pathway, we characterized daytime and...