A5102015650- Silk-based biomaterials and applications
- Viral Infectious Diseases and Gene Expression in Insects
- Invertebrate Immune Response Mechanisms
- Insect Resistance and Genetics
- Periodontal Regeneration and Treatments
- Animal Genetics and Reproduction
- RNA Interference and Gene Delivery
- Virology and Viral Diseases
- Bone Tissue Engineering Materials
- Antimicrobial Peptides and Activities
- Conducting polymers and applications
- Advanced Sensor and Energy Harvesting Materials
- Phytase and its Applications
- Chemical Synthesis and Analysis
- Infant Nutrition and Health
- Microbial infections and disease research
- vaccines and immunoinformatics approaches
- Collagen: Extraction and Characterization
- Blood groups and transfusion
- Electrospun Nanofibers in Biomedical Applications
- Osteoarthritis Treatment and Mechanisms
- Insect symbiosis and bacterial influences
- Tendon Structure and Treatment
- Silkworms and Sericulture Research
- Rabies epidemiology and control
Southwest University
2013-2024
Sanofi (China)
2024
The First People's Hospital of Tianmen
2022
State Key Laboratory of Silkworm Genomic Biology
2013-2022
Novel (United States)
2019-2021
Sericultural Research Institute
2019-2021
Xi'an University of Science and Technology
2019-2020
Chongqing University
2019
State Council of the People's Republic of China
2019
Abstract Fundamental and applied studies of silkworms have entered the functional genomics era. Here, we report a multi-gene expression system (MGES) based on 2A self-cleaving peptide (2A), which regulates simultaneous cleavage multiple gene targets in silk gland transgenic silkworms. First, glycine-serine-glycine spacer (GSG) was found to significantly improve efficiency 2A. Then, six types 2As with GSG analyzed. The shortest porcine teschovirus-1 (P2A-GSG) exhibited highest all insect cell...
Abstract With an increasing clinical demand for functional therapeutic proteins every year, there is requirement the massive production of bioactive recombinant human acidic fibroblast growth factor (r-haFGF). In this present study, we delicately explore a strategy mass r-haFGF protein with biological activity in transgenic silkworm cocoons. The sequence-optimized haFGF was inserted into enhanced sericin-1 expression system to generate original strain, which then further crossed PIG...
Human platelet derived growth factor (PDGF) is a major therapeutic protein with great demand in the clinical setting; however, its rate of supply far from meeting needs. Here, we provide an effective strategy to produce PDGF-BB large quantities using transgenic silkworm. The codon-optimized PDGF-B gene regulated by highly efficient sericin-1 expression system was integrated into genome high transcriptional silkworm competitively inhibited transcription endogenous which caused significant...
The present study demonstrates fabrication of PDGF-BB functionalized sericin hydrogel to explore biomaterials-related utility in bone tissue engineering.
Conventional hot-alkaline cocoon degumming techniques greatly weaken the physicochemical and mechanical properties of silk fibroin fiber, thus affecting quality fabric. Moreover, it causes massive energy waste serious environmental pollution.This study aims to establish a novel self-degumming method by genetic modification silkworm varieties fibers.The self-degummed material was generated specifically overexpressing trypsinogen protein in sericin layer thread; effect evaluated rate protein,...
Silk glands are used by silkworms to spin silk fibers for making their cocoons. These have recently been regarded as bioreactor hosts the cost-effective production of other valuable exogenous proteins and drawn wide attention.In this study, we established a transgenic silkworm strain which synthesizes recombinant human lactoferrin (rhLF) in gland spins them into cocoon our previously constructed based system. The yield rhLF with highest expression level was estimated be 12.07 mg/g shell...
In this article, polyaniline nanofibers/reduced graphene oxide (PANI-NFs/rGO) nanocomposites were prepared by a one-pot hydrothermal method. Under the condition of high temperature and pressure, (GO) was reduced to rGO aniline in-situ polymerized form PANI-NFs using ammonium persulfate as oxidant. The morphologies structures PANI-NFs/rGO characterized scanning electron microscopy, Fourier transform infrared spectroscopy (FTIR), Raman analysis. results show that uniformly grow on surfaces...
Functional biomaterials have great potential for future medical application. Although functional produced from genetically engineered silk are promising, it can be challenging to simultaneously maintain their structural stability and functionality. Herein, a stable sericin protein-based composite gel biomaterial with improved cell-proliferation activity was designed fabricated using material containing human-derived cytokines combined agarose molecules. This is porous consists of protein...
Functional silk mats with improved cell proliferation activity are promising medical materials to accelerate damaged wound healing and tissue repair. In this study, novel functional were fabricated from human fibroblast growth factor (FGF)-containing cocoons generated by expressing acid FGF1 basic FGF2 in silkworms. First, containing proteins alone or combination physically cutting genetically engineered cocoons. Compared those of normal mats, the physical properties these such as fibre...
Introduction Signal peptide peptidase ( SPP ) is an intramembrane protease involved in a variety of biological processes, it participates the processing signal peptides after release nascent protein to regulate endoplasmic reticulum associated degradation (ERAD) pathway, binds misfolded membrane proteins, and aids their clearance process. Additionally, regulates normal immune surveillance assists viral proteins. Although essential for many infections, its role silkworms remains unclear....
With the demand for more efficient and safer therapeutic drugs, targeted peptides are well received due to their advantages of high targeting (specificity), low immunogenicity, minimal side effects. However, conventional methods screening in natural proteins tedious, time-consuming, less efficient, require too many validation experiments, which seriously restricts innovation clinical development peptide drugs. In this study, we established a novel method proteins. We also provide details...
There is concrete evidence that lncRNA-MINCR involved in tumorigenesis of a number human cancers through modulation Wnt/β-catenin signaling pathway. However, the characterization regulatory role has not been worked out osteosarcoma yet. The present study was undertaken to explore osteosarcoma. tissues and cell lines were found exhibit significant (P<0.05) over-expression lncRNA-MINCR. Silencing cells suppressed their viability induction apoptosis. Saos-2 exhibited decline migration...
Abstract Background A serum-free, highly purified Vero rabies vaccine–next generation (PVRV-NG2) is under development. We conducted a phase III trial to describe the safety and immunogenicity profile of PVRV-NG2 compared with those licensed vaccine (PVRV) in simulated postexposure prophylaxis (PEP) Zagreb regimen Thailand. Methods Healthy adults aged ≥18 years (n = 201) were randomized 2:1 ratio receive or PVRV PEP (days 0, 7, 21 [2-1-1]) regimen, concomitant human immunoglobulin (HRIG) at...