A5058873539- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- HIV, Drug Use, Sexual Risk
- Rabies epidemiology and control
- Virology and Viral Diseases
- Liver Disease Diagnosis and Treatment
- Cervical Cancer and HPV Research
- HIV/AIDS Impact and Responses
- Tuberculosis Research and Epidemiology
- Genital Health and Disease
- Systemic Lupus Erythematosus Research
- Viral gastroenteritis research and epidemiology
- Reproductive tract infections research
- Genomics and Phylogenetic Studies
- Viral Infections and Outbreaks Research
- Vibrio bacteria research studies
- Public Health and Social Inequalities
- Sex work and related issues
- Dengue and Mosquito Control Research
- Machine Learning in Bioinformatics
- Chronic Disease Management Strategies
Universidade Nova de Lisboa
2014-2024
Fundación Instituto de Inmunología de Colombia
2014-2024
University of Applied and Environmental Sciences
2021-2024
Instituto de Medicina Tropical
2024
Sanofi (France)
2022-2024
Institut Mérieux (France)
2022
Universidad del Rosario
2012-2020
Instituto de Investigação Científica Tropical
2017
KU Leuven
2013-2015
Rega Institute for Medical Research
2013-2015
To investigate differences in pathogenesis, diagnosis and resistance pathways between HIV-1 subtypes, an accurate subtyping tool for large datasets is needed. We aimed to evaluate the performance of automated tools classify different subtypes circulating recombinant forms using pol, most sequenced region clinical practice. also present upgraded version 3 Rega HIV (REGAv3).HIV-1 pol sequences (PR+RT) 4674 patients retrieved from Portuguese Drug Resistance Database, 1872 trimmed full-length...
The HIV pandemic is characterized by extensive genetic variability, which has challenged the development of drugs and vaccines. Although genomes have been classified into different types, groups, subtypes recombinants, a comprehensive study that maps genome-wide diversity at population level still lacking to date. This aims characterize genomic in large-scale sequence populations, identify driving factors shape genome diversity. A total 2996 full-length sequences from 1705 patients infected...
A key challenge in the field of HIV-1 protein evolution is identification coevolving amino acids at molecular level. In past decades, many sequence-based methods have been designed to detect position-specific coevolution within and between different proteins. However, an ensemble system that integrates improve detection has not developed.We integrated 27 prediction published 2004 2013 into system. This allowed combinations for predictions. Using structures experimental data, we evaluated...
Since the ignition of HIV-1 group M pandemic in beginning 20th century, lineages have spread heterogeneously throughout world. Subtype C rapidly through sub-Saharan Africa and is currently dominant HIV lineage worldwide. Yet epidemiological evolutionary circumstances that contributed to its expansion remain poorly understood. Here, we analyse 346 novel pol sequences from DRC compare dynamics main lineages, subtypes A1, D. Our results place origins subtype 1950s Mbuji-Mayi, mining city...
We aimed to study epidemic trends and predictors for transmitted drug resistance (TDR) in our region, its clinical impact association with transmission clusters. included 778 patients from the AIDS Reference Center Leuven (Belgium) diagnosed 1998 2012. Resistance testing was performed using population-based sequencing TDR estimated WHO-2009 surveillance list. Phylogenetic analysis maximum likelihood Bayesian techniques. The cohort predominantly Belgian (58.4%), men who have sex (MSM)...
Abstract Background A next-generation Vero cell rabies vaccine (PVRV-NG2) was developed using the same Pitman–Moore strain as in licensed purified (PVRV; Verorab) and human diploid (HDCV; Imovax Rabies®). Methods This dual-center, modified, double-blind, phase 3 study evaluated immunogenic non-inferiority safety of PVRV-NG2 with without concomitant intramuscular immunoglobulin (HRIG) versus PVRV + HRIG HDCV a simulated post-exposure prophylaxis (PEP) regimen. Healthy adults ≥18 years old (N...
Abstract Objectives To identify the clinical and demographic characteristics of HIV ‐positive ‐negative women infected by multiple HPV types. Methods 1399 participated in study (240 1159 women). Samples were provided for Pap tests detection typing PCR . Data collected on infection, frequency type distribution. Odds ratios reported from logistic regression models. Results Compared with women, had higher frequencies cervical abnormality (30% vs 20.8%), prevalence (68.3% 51.3%) more commonly...
Abstract: Historically, human migrations have determined the spread of many infectious diseases by promoting emergence temporal outbreaks between populations. We aimed to analyze health indicators, expenditure, and disability caused tuberculosis (TB) HIV/AIDS burden under Colombian-Venezuelan migration flow focusing on Northeastern border. A retrospective study was conducted using TB data since 2009. consolidated a database official reports from Colombian Surveillance System, World Health...
HIV infection leads to a decreasing immune response, thereby facilitating the appearance of other infections, one most important ones being HPV. However, studies are needed for determining associations between immunodeficiency caused by and/or presence HPV during course cervical lesions and their degree malignancy. This study describes cytological findings revealed Papanicolaou test, laboratory characteristics molecular profile in women with without infection. A total 216 HIV-positive 1,159...
In-depth phylogeographic analysis can reveal migration patterns relevant for public health planning. Here, as a model, we focused on the provenance, in current Italian HCV subtype 1a epidemic, of NS3 resistance-associated variant (RAV) Q80K, known to interfere with action NS3/4A protease inhibitor simeprevir. HCV1a were analysed using Bayesian phylodynamic tools, capitalising newly generated and publicly available time geo-referenced encoding virus genetic sequence data. Our results showed...
Abstract Background Estimation of temporal changes in human immunodeficiency virus (HIV) transmission patterns can help to elucidate the impact preventive strategies and public health policies. Methods Portuguese HIV-1 subtype B G pol genetic sequences were appended global reference data sets identify country-specific clades. Bayesian birth-death models used estimate subtype-specific effective reproductive numbers (Re). Discrete trait analysis (DTA) was quantify mixing among groups. Results...
The latest nonnucleoside reverse transcriptase inhibitor (NNRTI) rilpivirine (RPV) is indicated for human immunodeficiency virus type-1 (HIV-1) patients initiating antiretroviral treatment, but the extent of genotypic RPV resistance in treatment-naive outside clinical trials poorly defined.This retrospective observational study data from Belgium and Portugal evaluates information HIV-1 drug-naive obtained purpose drug testing. Rilpivirine resistance-associated mutations (RPV-RAMs) were...
Background Molecular epidemiology techniques allow us to track the HIV-1 transmission dynamics. Herein, we combined genetic, clinical and epidemiological data collected during routine treatment evaluate dynamics characteristics of clusters most prevalent subtypes in state São Paulo, Brazil. Methods This was a cross-sectional study conducted with 2,518 persons living HIV (PLWH) from 53 cities Paulo between Jan 2004 Feb 2015. The phylogenetic tree protease/reverse transcriptase (PR/RT) regions...
Co-infections with HIV and HCV are very frequent among people who inject drugs (PWID). However, few studies comparatively reconstructed the transmission patterns of both viruses in same population. We have recruited 117 co-infected PWID during a recent outbreak Romania. Phylogenetic analyses were performed on sequences order to characterize compare dynamics two viruses. Three large clusters (2 subtype F1 one CRF14_BG) thirteen smaller networks (genotypes 1a, 1b, 3a, 4a 4d) identified. Eighty...
Abstract Since HIV-1 treatment is increasingly considered an effective preventionstrategy, it important to study local epidemics formulate tailored preventionpolicies. The prevalence of in Cyprus was historically low until 2005. To investigatethe shift epidemiological trends, we studied the transmission dynamics Cyprususing a densely sampled Cypriot cohort that included 85 percent ofHIV-1-infected individuals linked clinical care between 1986 and 2012 based on detailedclinical,...
Migration is associated with HIV-1 vulnerability. Objectives: To identify long-term trends in molecular epidemiology and antiretroviral drug resistance (ARV) among migrants followed up Portugal Methods: 5177 patients were included between 2001 2017. Rega, Scuel, Comet, jPHMM algorithms used for subtyping. Transmitted (TDR) Acquired (ADR) defined as the presence of surveillance mutations (SDRMs) IAS-USA 2015 algorithm, respectively. Statistical analyses performed. Results: subtypes infecting...
Treatment for All recommendations have allowed access to antiretroviral (ARV) treatment an increasing number of patients. This minimizes the transmission infection but can potentiate risk transmitted (TDR) and acquired drug resistance (ADR).
Objective: To investigate the dynamics of phylogenetic transmission clusters involving immigrants Portuguese Speaking Countries living in Portugal. Design/methods: We included genomic sequences, sociodemographic and clinical data from 772 HIV migrants followed Portugal between 2001 2017. reconstruct HIV-1 clusters, we applied inference 16 454 patients: migrants, 2973 12 709 global controls linked to demographic data. Transmission were defined using: with SH greater than 90% (phylogenetic...
Background Portugal has one of the most severe HIV-1 epidemics in Western Europe. Two subtypes circulate parallel since beginning epidemic. Comparing their transmission patterns and its association with transmitted drug resistance (TDR) is important to pinpoint hotspots develop evidence-based treatment guidelines. Methods Demographic, clinical genomic data were collected from 3599 naive patients between 2001 2014. Sequences obtained testing used for subtyping, TDR determination clusters (TC)...
Abstract The World Health Organization protocol for rabies post-exposure prophylaxis (PEP) recommends extensive wound washing, immediate vaccination, and administration of immunoglobulin (RIG) in severe category III exposures. Some studies have shown that RIG can interfere with vaccine immunogenicity to some extent. We investigated the interference on a next generation highly purified Vero cell candidate (PVRV-NG) versus standard-of-care vaccines previously described hamster model. either...
A serum-free, highly purified rabies vaccine produced in Vero cells is under development. The initial formulation, PVRV-NG, was evaluated five Phase II studies and subsequently reformulated (PVRV-NG2). This multicenter, observer-blinded study investigated the safety immune response of three different doses (antigen content) PVRV-NG2 versus a licensed human diploid cell (HDCV; Imovax rabies®). Healthy adults (N = 320) were randomized to receive (low, medium, or high dose), HDCV (2:2:2:1:1...