A5065803694- Vasculitis and related conditions
- PI3K/AKT/mTOR signaling in cancer
- Systemic Lupus Erythematosus Research
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Animal Virus Infections Studies
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- Chronic Lymphocytic Leukemia Research
- Sarcoidosis and Beryllium Toxicity Research
- Renal Transplantation Outcomes and Treatments
- Protein Kinase Regulation and GTPase Signaling
- Renal Diseases and Glomerulopathies
- Immunotherapy and Immune Responses
- Sphingolipid Metabolism and Signaling
- Genomics, phytochemicals, and oxidative stress
- Bone health and treatments
- Otitis Media and Relapsing Polychondritis
- Medical Imaging and Pathology Studies
- Hematopoietic Stem Cell Transplantation
- HIV Research and Treatment
- Parvovirus B19 Infection Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Invertebrate Immune Response Mechanisms
- Organ Donation and Transplantation
SUNY Upstate Medical University
2013-2025
Centro Hospitalar de Entre o Douro e Vouga E.P.E.
2024
Centro Hospitalar de Lisboa Ocidental
2022-2024
Hospital Braga
2024
Centro Hospitalar do Tâmega e Sousa
2024
Centro Hospitalar de Lisboa Central
2024
San Antonio College
2024
Centro Hospitalar do Porto
2024
Leipzig University
2022
Universidade Federal de Viçosa
2022
We show here that both SHIP1 (Inpp5d) and its paralog SHIP2 (Inppl1) are expressed at protein level in microglia. To examine whether targeting of SHIP paralogs might influence microglial physiology function, we tested the capacity SHIP1-selective, SHIP2-selective pan-SHIP1/2 inhibitors for their ability to impact on microglia proliferation, lysosomal compartment size phagocytic function. find highly potent can significantly increase size, phagocytosis dead neurons amyloid beta (Aβ)1-42 by...
Porcine parvovirus (PPV) is a major cause of reproductive failure in swine. The mechanisms implicated the first steps infection that lead to delivery PPV genome nucleus are poorly understood. In present work, panel chemical inhibitors was used dissect cellular involved establishing infection. results demonstrated following binding sialic acids on cell surface glycoproteins, virus both clathrin-mediated endocytosis and macropinocytosis pathways gain access into cells. Virus obtained from...
Abstract Background We evaluated the prevalence of transmitted drug resistance (TDR) to integrase strand-transfer inhibitors (INSTIs) and nucleoside/nucleotide reverse transcriptase (NRTIs) clinically relevant (CRR) in newly diagnosed people with human immunodeficiency virus (HIV; PWH) naive antiretroviral therapy (ART) Europe. Methods MeditRes is a consortium that includes ART-naive PWH France, Greece, Italy, Portugal, Spain during 2018–2021. Reverse INSTI sequences were provided by...
Background: Hemoglobinopathies are the most common group of genetic disorders worldwide.They mainly result from autosomal recessive transmission mutated genes behind synthesis globin chains, and fall into two main groups: thalassemia syndromes, structural haemoglobin (Hb) variants.Although some Hb variants silent in heterozygotes, they can be severe homozygous forms.Aim: To analyse incidence hemoglobinopathies all samples studied both for guided screening incidental finding, last 5 years to...
Abstract Dectin-1 (Clec7a) is a paradigmatic C-type lectin receptor that binds Syk through hemITAM motif and couples sensing of pathogens such as fungi to induction innate responses. engagement triggers plethora activating events, but little known about the modulation pathways. Trying define more precise picture early signaling, we explored interactome intracellular tail in mouse dendritic cells. We found unexpected binding SHIP-1 phosphatase phosphorylated hemITAM. colocalized with during...
Intermittent inhibition of the phosphatase SHIP1 promotes antitumor immunity and memory in mouse models lymphoma colon cancer.
Here, we describe the use of artificial intelligence to identify novel agonists SH2-containing 5' inositol phosphatase 1 (SHIP1). One compounds, K306, represents most potent agonist identified date. We find that K306 exhibits selectivity for SHIP1 vs. paralog enzyme SHIP2, and this activation does not require C2 domain which other known require. Thus, a new class with mode agonism. Importantly, can suppress induction inflammatory cytokines iNOS in macrophages or microglia, but by their...
The SH2‐containing inositol phosphatase (SHIP) has become an actively researched therapeutic target for a number of disorders, including Alzheimer's Disease, Graft‐vs‐Host disease, obesity and cancer. Analogs the aminosteroid SHIP inhibitor 3a‐aminocholestane (3AC) have been synthesized tested. with improved water solubility identified. Deletion C17 alkyl group from cholestane skeleton improves solubility, however these compounds inhibit both SHIP1 SHIP2. Enzyme kinetics imply that molecules...
Abstract Porcine plasma and factor VIII preparations were screened for parvovirus 4 (PARV)–like viruses. Although the prevalence of PARV4-like viruses in samples was relatively low, appeared to be concentrated during manufacture VIII. from human porcine origins coevolved likewise with their hosts.
ABSTRACT Nuclear targeting of capsid proteins (VPs) is important for genome delivery and precedes assembly in the replication cycle porcine parvovirus (PPV). Clusters basic amino acids, corresponding to potential nuclear localization signals (NLS), were found only unique region VP1 (VP1up, part). Of five identified regions (BR), three VP1up: BR1 was a classic Pat7 NLS, combination BR4 BR5 bipartite NLS. These NLS essential viral replication. VP2, major protein, lacked these contained no with...
In our previous study, we observed a severe reduction in the Src Homology 2-containing-inositol-phosphatase-1 (SHIP1) protein subpopulation of subjects from small adult Crohn's Disease (CD) cohort. This pilot study had been undertaken since previously demonstrated that engineered deficiency SHIP1 mice results spontaneous and CD-like ileitis. Here extend analysis expression peripheral blood mononuclear cells second much larger Inflammatory Bowel (IBD) cohort, comprised both CD Ulcerative...
ABSTRACT Factors controlling porcine parvovirus (PPV) replication efficiency are poorly characterized. Two prototype strains of PPV, NADL-2 and Kresse, differ greatly in pathogenic capacity both vivo vitro , yet their genomic sequence is nearly identical (13 single-nucleotide substitutions a 127-nucleotide noncoding repeated sequence). We have created series chimeras these to identify the genetic elements involved host cell line. While capsid proteins ultimately determine viral fitness,...
Hematopoietic stem cell transplantation (HSCT) is a highly effective procedure enabling long-term survival for patients with hematologic malignancy or heritable defects. Although there has been dramatic increase in the success rate of HSCT over last two decades, can result serious, sometime untreatable, disease due to toxic conditioning regimens, graft vs. host and required use mismatched bone marrow some cases. Studies utilizing germline knockout mice have discovered several candidate genes...