// Leong-Perng Chan 1, 2 , Cheng Liu 3, 4 Feng-Yu Chiang Ling-Feng Wang Ka-Wo Lee Wan-Ting Chen 5 Po-Lin Kuo 6 and Chia-Hua Liang 1 Graduate Institute of Clinical Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan Department Otolaryngology-Head Neck Surgery, University Hospital, 3 Division Plastic Surgery & HBOT Center, Chi Mei Tainan, Electrical Engineering, Southern Science Technology, Cosmetic Science, Chia Nan Pharmacy National Sun Yat-Sen Correspondence to: Kuo, email:...

Tumor exploitation: Novel monomethylauristatin E and didesmethylauristatin prodrugs were prepared, in vitro vivo evaluations of the using human mouse cancer cell lines showed that they are less toxic more efficacious than parent cytotoxins, as their activation was catalyzed selectively by cysteine protease, legumain, which is overexpressed active form tumor microenvironments.

Long non-coding RNA (lncRNA) XIST has been implicated in the progression of a variety tumor diseases. The purpose this study was to explore molecular role lncRNA human hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC).

BACKGROUND Ovarian cancer has the highest mortality rate among all female genital tumors because of its insidious onset and drug resistance. Hypoxia-inducible factor 1alpha (HIF-1alpha), one best-studied oncogenes, plays an important part in tumor adaptation to microenvironmental hypoxia was found be overexpressed several malignancies, including ovarian cancer. Previous studies that effect HIF-1alpha on cancers may correlated with autophagy some signaling pathways, such as PI3K/AKT/mTOR,...

lncRNA cancer susceptibility candidate 2 (CASC2) is a recently identified oncogenic in different types of cancers. Our preliminary microarray data showed that CASC2 was downregulated the plasma patients with rheumatoid arthritis (RA), indicating involvement this RA. In present study, and IL‑17 were detected by reverse transcription‑­quantitative PCR ELISA, respectively. Diagnostic analyses performed using receiver operating characteristic curves. Flow cytometry to evaluate cell apoptosis....

We investigated whether autophagy-related long noncoding RNAs (lncRNAs) can predict prognosis in bladder cancer. obtained cancer lncRNA data from The Cancer Genome Atlas and genes the Human Autophagy Database. Fifteen lncRNAs with prognostic significance were identified. Multivariate Cox analysis was used to construct a risk score model, which divided patients into high-risk low-risk groups. found that group had better survival than those group. Subgroup showed also worse OS subgroups based...

Abstract Hypoxic–ischemia injury occurs after trauma causes consequential bone necrosis. Non‐steroid anti‐inflammatory drugs (NSAIDs) are frequently used in orthopedic clinics for pain relief. However, the underlying mechanism and outcome usage of NSAIDs is poorly understood. To investigate damage loss osteoblast function hypoxia, two hypoxia mimetics, cobalt chloride (CoCl 2 ) desferrioxamine (DFO), were to create an vitro hypoxic microenvironment. The cell was observed by decreases...

Background: Much evidence unveils the significance of long non-coding RNAs (lncRNAs) in diverse cancers. This study was designed to clarify function and mechanism lncRNA GATA6 antisense RNA 1 (GATA6-AS1) progression non-small cell lung cancer (NSCLC). Methods: GATA6-AS1, miR-543 Raf kinase inhibitor protein ( RKIP ) mRNA expressions were detected by qRT-PCR. Chi-square test adopted analyze relationship between GATA6-AS1 expression clinicopathological parameters NSCLC patients. cells H1299...

The effects of epigenetic modifiers have been uncovered on cellular reprogramming and, specifically, sustaining characteristics cancer stem cells. We here aim to investigate whether lysine-specific demethylase 1 (LSD1) affects the development oral squamous cell carcinoma (OSCC) by cells from OSCC (OSCSCs).RT-qPCR detection was firstly conducted screen out research gene determining differential expression histone demethylases and methylases in identified OSCSCs. Then, microarray analysis...

Analysis of genetic changes is often hampered by insufficient starting DNA from limited clinical tissue specimens. We employed ligation-mediated PCR (LM-PCR) for global amplification the genome to overcome this limitation, generating up 5 microg representative amplicons genomic as little one cell. demonstrate successful in high-quality source like laser-captured cultured cells, well partially degraded old formalin-fixed paraffin-embedded sections. This process generates adaptor-tailed...

The importance of microRNAs in regulating osteosarcoma development has been studied recent years. However, the function microRNA-451a growth is rarely investigated. Here, we explored expression cell lines. Bioinformatic software, luciferase activity reporter assay, and Western blot were conducted to determine association between tripartite motif-containing 66. Cell Counting Kit-8 assay transwell used explore regulatory effects on cells. Moreover, whether modulates biological by was found be...

There is concrete evidence that lncRNA-MINCR involved in tumorigenesis of a number human cancers through modulation Wnt/β-catenin signaling pathway. However, the characterization regulatory role has not been worked out osteosarcoma yet. The present study was undertaken to explore osteosarcoma. tissues and cell lines were found exhibit significant (P<0.05) over-expression lncRNA-MINCR. Silencing cells suppressed their viability induction apoptosis. Saos-2 exhibited decline migration...

<title>Abstract</title> Background Early detection of prostate cancer (PCa) can improve the prognosis patients. Currently, role specific antigen test for PCa screening remains debatable. We aimed to develop an efficient and clinically applicable method by noninvasive several serum miRNA levels. Methods A mixed cohort, including PCa, multiple other cancers (OCa), benign disease (BPD) health population (HP), study with 8,741 samples was conducted. Six machine learning algorithms were employed...

Abstract Hypoxia is a primary inducer of cardiomyocyte injury, its significant marker being hypoxia-induced apoptosis. Nuclear respiratory factor-1 (NRF-1) and hypoxia-inducible factor (HIF)-1α are transcriptional regulatory elements implicated in multiple biological functions, including oxidative stress response. However, their roles apoptosis remain unknown. The effect HIF-α, together with NRF-1, exerts on also remains unclear. We established myocardial hypoxia model investigated the...

Background: Dysregulated HMMR is linked to prostate cancer (PCa) prognosis, the precise mechanisms remain unclear. Here, we sought elucidate role of in PCa progression as well underlying mechanisms.Methods: The expression clinical samples were detected through western blotting and qRT-PCR, further verified immunohistochemistry (IHC) a large cohort. Cell viability, cell cycle, migration invasion assessed by Counting Kit-8 (CCK8) assay, flow cytometry detection Transwell assay respectively....

Abstract Background: Hypoxia is a primary inducer of cardiomyocyte injury, its significant marker being hypoxia-induced apoptosis. Nuclear respiratory factor-1 (NRF-1) and hypoxia-inducible factor-1α (HIF-1α) are transcriptional regulatory elements implicated in multiple biological functions, including oxidative stress response. However, their roles apoptosis remain unknown. The effect HIF-α, together with NRF-1, exerts on also remains unclear. Methods: We established myocardial hypoxia...

Abstract Hypoxia is a primary inducer of cardiomyocyte injury, its significant marker being hypoxia-induced apoptosis. Nuclear respiratory factor-1 (NRF-1) and hypoxia-inducible factor (HIF)-1α are transcriptional regulatory elements implicated in multiple biological functions, including oxidative stress response. However, their roles apoptosis remain unknown. The effect HIF-α, together with NRF-1, exerts on also remains unclear. We established myocardial hypoxia model investigated the...