A5013109810- Cardiomyopathy and Myosin Studies
- Congenital heart defects research
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Biomedical Ethics and Regulation
- Muscle Physiology and Disorders
- Neurogenetic and Muscular Disorders Research
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Physical Activity and Health
- Coronary Interventions and Diagnostics
- Cardiac electrophysiology and arrhythmias
- Polysaccharides and Plant Cell Walls
- Viral Infections and Outbreaks Research
- Advanced Chemical Sensor Technologies
- Antiplatelet Therapy and Cardiovascular Diseases
- Cardiac pacing and defibrillation studies
- Heart Failure Treatment and Management
- Cellular transport and secretion
- Ocular Surface and Contact Lens
- Signaling Pathways in Disease
- Fire effects on ecosystems
- Circular RNAs in diseases
- Genetics and Neurodevelopmental Disorders
- Viral Infections and Vectors
First Affiliated Hospital of Zhengzhou University
2018-2025
Zhengzhou University
2020-2021
Galectin-3, a β-galactoside-binding lectin, is abnormally increased in cardiovascular disease. Plasma Galectin-3 receives Class II recommendation for heart failure management and has been extensively studied multiple cellular functions. The direct effects of on platelet activation remain unclear. This study explores the thrombosis.A strong positive correlation between plasma concentration aggregation or whole blood thrombus formation was observed patients with coronary artery disease (CAD)....
To investigate the combined effect of albumin (ALB) and globulin (GLB) on overall survival (OS) patients with heart failure (HF).Retrospective cohort study.A hospital.404 first diagnosed HF.Serum ALB GLB were measured within 3 days after admission. The to ratio (AGR) was calculated as divided by GLB. receiver operating characteristic curve used calculate cut-off points for ALB, AGR. Patients low levels (≤35.3 g/L) high (>27.0 assigned an albumin-globulin score (AGS) 2, those only one two...
Abstract Pleckstrin homology domain-containing family M member 2 (PLEKHM2) is an essential adaptor for lysosomal trafficking and its homozygous truncation have been reported to cause early onset dilated cardiomyopathy (DCM). However, the molecular mechanism of PLEKHM2 deficiency in DCM pathogenesis progression poorly understood. Here, we generated vitro model knockout (KO) induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) elucidate potential pathogenic PLEKHM2-deficient...
Highlights•SLC25A3 deficiency causes myocardial hypertrophy and Ca2+ homeostasis imbalance•SLC25A3 leads to impaired ATP synthesis enhanced glycolysis•Missense mutations in SLC25A3 produce similar disease phenotypes deficiency•Mitochondrial transplantation improves energy metabolism restores homeostasisSummarySLC25A3 encodes mitochondrial phosphate carrier (PiC), which is involved inorganic transport. Clinical reports have found that most patients with homozygous or complex heterozygous...
Hypertrophic cardiomyopathy (HCM), the most common inherited heart disease, is frequently caused by mutations in β-cardiac myosin heavy chain gene (MYH7). Abnormal calcium handling and diastolic dysfunction are archetypical features of HCM MYH7 mutations. However, mechanism how leads to these remains unclear, which inhibits development effective therapies. Initially, cardiomyocytes were generated from induced pluripotent stem cells an eight-year-old girl diagnosed with carrying a MYH7(C.1063...
Hypertrophic cardiomyopathy (HCM) is a heterogeneous myocardial disease often caused by sarcomeric gene mutations. MYH7 one of the most common genes associated with HCM. In this study, we generated human induced pluripotent stem cell (iPSC) line ZZUNEUi020-A from peripheral blood mononuclear cells female HCM patient p. R719Q (c. 2156G > A) mutation in MYH7. This expressed pluripotency markers, showed normal karyotype and could differentiate into all three germ layers vitro.
In this study, we report a human induced pluripotent stem cell (iPSC) line from healthy 27-year-old female individual using non-integrative Sendai viral reprogramming technology. The expresses stemness markers, exhibits normal karyotype, and can differentiate into three germ layers in vivo. This iPSC provides control group for studying disease mechanisms, drug screening, toxicity testing.
Exposure to ambient pollutants and chemicals were found be associated with increased risk of hypertension. However, the relationship between aldehyde exposure hypertension are still unclear. This study aimed investigate potential associations serum aldehydes levels prevalent hypertension.A total 1,733 U.S. adults data on outcome measurement from National Health Nutrition Examination Survey 2013-2014 included. The measured via an automated analytical method using solid phase microextraction...
Urine cells (or renal tubular cells) can be isolated from human urine samples efficiently. This noninvasive and cost-effective method to collect biological sample provide us favorable access donor human. In the present study, we generate ZZUNEUi022-A, a cells-derived induced pluripotent stem cell (iPSC) line, 29-year-old healthy male via Sendai virus delivery system. ZZUNEUi022-A showed stable karyotype, could differentiate into three germ layers (ectoderm, mesoderm, endoderm) readily in an...
<title>Abstract</title> We conducted circRNA sequencing of endothelial cells (ECs) subjected to hypoxia and serum deprivation ascertain molecules linked partial or transient mesenchymal activation in following tissue ischemia/hypoxia. The outcome demonstrated that the circATXN1 expression was significantly raised. Herein, we estimated function regulating characteristics during endothelial-to-mesenchymal transition (EndMT) its impact on angiogenesis. Over-expression inhibited traits cells,...
Abstract BACKGROUND The non-exercise estimated cardiorespiratory fitness (eCRF) has been recognized as an important predictor of mortality among the general population. This study sought to evaluate relationship between eCRF and from all causes, cardiovascular disease (CVD), cancer in hypertensive adults. METHODS We included 27,437 adults with hypertension National Health Nutrition Examination Survey (NHANES) III 10 NHANES cycles 1999 2018. Multivariate Cox proportional hazard models were...
Hypertrophic cardiomyopathy (HCM) is the most common monogenic cardiovascular disorder. In this study, we generated human induced pluripotent stem cells (iPSC) ZZUNEUi007-A from dermal fibroblasts of an HCM patient with p. R663H (c. 1988 G > A) mutation in MYH7. The hiPSC line had normal karyotype, showed robust expression pluripotency markers and could differentiate into all three germ layers vivo.
The transient receptor potential cation channel subfamily M member 8 (TRPM8) is a kind of non-selective which controls Ca2+ homeostasis. Mutations in TRPM8 were related to dry eye diseases (DED). Here we constructed knockout cell line WAe009-A-A from the original embryonic stem H9 using CRISPR/Cas9 technology, maybe helpful for exploring pathogenesis DED. cells possess morphology and pluripotency as well normal karyotype, have ability differentiating into three germ layers vitro.
An induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMCs) of a 41-year-old male patient with hypertrophic cardiomyopathy who carries G3755A heterozygote mutation in the MYH6 gene. The iPSC expressed pluripotency markers, exhibited normal karyotype, presented specific mutation, and demonstrated differentiation potential into three germ layers vitro.
HCM is one of the most common inheritable cardiac disease. In our study, we established a human-induced pluripotent stem cells (hiPSCs) line (ZZUNEUi016-A) from hypertrophic cardiomyopathy patient with pathogenic heterozygote mutation in MYH7 gene. ZZUNEUi016-A expressed pluripotency markers normal karyotype and showed ability to differentiate into all three germ layers vitro.
A 25-years-old hypertrophic cardiomyopathy male patient donated his peripheral blood mononuclear cells (PBMCs) with heterozygote mutation in theTNNT2 gene. We generated induced pluripotent stem cell (iPSC) normal karyotypic and expressing NANOG, Lin28, GDF3 DNMT3. The iPSC line has demonstrated pluripotency by differentiating into three germ layers vitro. ZZUNEUi021-A would serve as an vitro model for loss of TNNT2 function.
Induced pluripotent stem cells (iPSCs) have differentiation potential into different somatic cell types in vitro and are a useful tool to investigate pathomechanistic cellular processes. In this study, we generated human induced (iPSC) ZZUNEUi012-A from an apparently healthy female individual using integration-free reprogramming method. The hiPSC line was had normal karyotype, showed robust expression of pluripotency markers could differentiate all three germ layers vitro.
Dilated cardiomyopathy (DCM) is the commonest type of cardiomyopathy. In this study, peripheral blood mononuclear cells (PBMCs) were isolated from a DCM patient with p. Glu12513fs(c.37537delG) mutation in TTN and reprogrammed to human induced pluripotent stem (iPSCs). The ZZUNEUi017-A iPSC line expressed pluripotency markers, exhibiting normal male karyotype (46, XY) demonstrating differentiation potential into three germ layers vitro.
Hypertrophic cardiomyopathy is the commonest monogenic in humans and was reported to be associated with ALPK3 gene mutation. We report generation characterization of human induced pluripotent stem cell (iPSC) line ZZUNEUi015-A, which derived from a patient heterozygous mutation (c.1013 T > C) diagnosed hypertrophic cardiomyopathy. The ZZUNEUi015-A maintains morphology cells, has pluripotency normal karyotype, differentiated into three germ layers vitro.
Induced pluripotent stem cells (iPSCs) can be used to generate different types of somatic in vitro and are a useful tool for investigating drug disease mechanisms. Here, we generated human induced cell (iPSC) line ZZUNEUi009-A from an apparently healthy 28-year-old female by reprogramming peripheral blood mononuclear with non-integrating vector. The iPSCs was pluripotent, maintained stable karyotype, could the three layers (ectoderm, mesoderm, endoderm) vitro.