A5036020802- Chronic Obstructive Pulmonary Disease (COPD) Research
- Asthma and respiratory diseases
- Respiratory and Cough-Related Research
- Neuroscience and Neuropharmacology Research
- Respiratory Support and Mechanisms
- Delphi Technique in Research
- Pediatric health and respiratory diseases
- COVID-19 Clinical Research Studies
- Ion channel regulation and function
- GABA and Rice Research
- COVID-19 and healthcare impacts
- Mass Spectrometry Techniques and Applications
- Drug Transport and Resistance Mechanisms
- Physiological and biochemical adaptations
- Amino Acid Enzymes and Metabolism
- Inhalation and Respiratory Drug Delivery
- Advanced Chemical Sensor Technologies
- Photoreceptor and optogenetics research
- Musculoskeletal pain and rehabilitation
- DNA and Nucleic Acid Chemistry
- Cannabis and Cannabinoid Research
- Neonatal Respiratory Health Research
- Pharmacological Receptor Mechanisms and Effects
- Cardiovascular and exercise physiology
- Intensive Care Unit Cognitive Disorders
Ospedale San Paolo
2024
Istituti Clinici Scientifici Maugeri
2010-2021
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2021
University Orthopedics Center
2021
Institute for Transfusion Medicine
2021
Fondazione Salvatore Maugeri
2009-2015
University of Insubria
2007-2012
The effect of pulmonary rehabilitation (PR) on the EuroQol Group's 5-dimension questionnaire (EQ-5D) in COPD has been poorly investigated. In addition, conflicting results were reported about visual analog scale component EQ-5D (EQ-VAS). purpose this study was to evaluate responsiveness EQ-VAS PR and its relationship with clinical functional parameters subjects COPD, as well define minimal clinically important difference (MCID) estimate for after PR.A total 468 in-patients stable...
Background: Individuals with COPD may present reduced peripheral muscle strength, leading to impaired mobility. Comprehensive pulmonary rehabilitation (PR) should include strength training, in particular lower limbs. Furthermore, simple tools for the assessment of performance are required. Objectives: To assess patients by sit-to-stand test (STST), as compared one-repetition maximum (1-RM), considered gold standard assessing non-laboratory situations, and evaluate responsiveness STST a PR...
The sit-to-stand (STS) test is a feasible tool for measuring peripheral muscle strength of the lower limbs. There evidence increasing use STS tests in patients with COPD. We sought to evaluate subjects COPD minimum clinically important difference 30-s after pulmonary rehabilitation.Stable undergoing and 6-min walk (6MWT) before rehabilitation were included. Responsiveness was determined by change results (Δ STS) rehabilitation. evaluated using an anchor-based method.96 moderate-to-severe At...
<b><i>Background:</i></b> International guidelines recommend the inclusion of patients with bronchiectasis in pulmonary rehabilitation (PR) to improve exercise capacity and health-related quality life (HRQoL). At present, effect PR these has been poorly investigated. <b><i>Objective:</i></b> The aim our retrospective analysis was evaluate effects predictors success for a program not related cystic fibrosis (non-CF bronchiectasis)....
Background: Bronchial hyperresponsiveness (BHR), sputum eosinophilia, and bronchial reversibility are often thought to be a hallmark of asthma, yet it has been shown occur in COPD as well. Objectives: To evaluate the relationship between BHR, lung function, airway inflammation patients. Methods: Thirty-one, steroid-free patients with stable, mild moderate were studied. The following tests carried out: baseline reversibility, provocative dose methacholine causing 20% fall forced expiratory...
Airway eosinophilic inflammation is a characteristic of asthmatic patients and sub group COPD subjects. Blood eosinophils are deemed as good surrogate marker sputum inflammation; however, controversial data have been published particularly in COPD. The aim our study was to compare blood "real life". Sputum induced stable with or asthma hypertonic saline solution were evaluated. Frequency comorbidities recorded. Correlations performed stratifying by disease comorbidities. 146 patients, 57 89...
There is, so far, no universal definition of severe asthma. This usually relies on: number exacerbations, inhaled therapy, need for oral corticosteroids, and respiratory function. The use such parameters varies in the different definitions used. Thus, according to chosen, each patient may result having asthma or not. aim this study was evaluate how choice a specific can change allocation patients.Data collected from Severe Asthma Network Italy (SANI) registry were analyzed. All patients...
Background: Pulmonary Rehabilitation (PR) is a multimodal treatment that still poorly investigated in severe asthma where respiratory symptoms remain "uncontrolled" despite intensive pharmacological therapy. Bronchiectasis and obstructive sleep apnea (OSAS) are common comorbidities which may worsen control.Aim: Aim of the present study to investigate effectiveness PR on functional exercise, dyspnea, muscle fatigue patients with asthma.Methods: A total 317 affected from according GINA...
<h3>BACKGROUND:</h3> Asthma is defined by airway inflammation associated with various respiratory symptoms, and pharmacologic treatment based on inhaled corticosteroids bronchodilators. Physical activity, educational training, nutritional support, psychological counseling are considered part of non-pharmacologic treatment; however, studies so far have investigated the effect single treatment. There few that demonstrate comprehensive pulmonary rehabilitation, but no clear data available...
Synaptic glycine levels are controlled by GLYTs (glycine transporters). GLYT1 is the main regulator of synaptic concentrations and catalyses Na+-Cl--glycine co-transport with a 2:1:1 stoichiometry. In contrast, neuronal GLYT2 supplies to presynaptic terminal 3:1:1 We subjected homology models molecular dynamics simulations in presence Na+. Using interaction potential maps silico mutagenesis, we identified conserved region external vestibule likely be involved Na+ interactions. Replacement...
The role of intracellular ions on the reverse GABA transport by neuronal transporter GAT1 was studied using voltage-clamp and [(3)H]GABA efflux determinations in Xenopus oocytes transfected with heterologous mRNA. Reverse induced injections measured terms net outward current generated transporter. Changes various ionic conditions affected current: higher concentrations Na(+) enhanced ratio over inward current, while a considerable decrease parallel reduction transporter-mediated were...
The sit-to-stand test (STST) is a feasible alternative for measuring peripheral muscle strength of the lower limbs. Our aim was to evaluate, in Chronic Obstructive Pulmonary Disease (COPD) patients, minimal clinically important difference (MCID) 30-second STST (30-STST) after pulmonary rehabilitation (PR). Stable COPD inpatients undergoing 30-STST and 6-minute walk (6MWT) before PR were included. Responsiveness determined by pre-to-post (∆) evaluation 30-STST. MCID evaluated using an...
BACKGROUND: The 6‐min walking test (6MWT) is responsive to physiological changes and pulmonary rehabilitation (PR) in patients with asthma. minimal clinically important difference (MCID) has not been established yet. OBJECTIVE: To determine the MCID of 6MWT METHODS: Using perceived change ability modified Medical Research Council (mMRC) score as anchors, receiver operating characteristic curves quantile regression, we evaluated before after PR these patients. St George Respiratory...
Electrophysiological and biophysical analyses were used to compare the partial complete transport cycles of intestinal oligopeptide transporter PepT1 among three species (seabass, zebrafish rabbit). On whole, presteady-state currents fish transporters similar each other. Rabbit differed from by having slower-decaying currents, charge vs. potential (Q/V) time constant (τ/V) curves shifted more positive potentials. All isoforms similarly affected external pH, showing acidity-induced slowing...
The oligopeptide transporter PepT1 is a protein found in the membrane of cells intestinal walls, and represents main route through which proteic nutrients are absorbed by organism. Along polypeptidic chain this protein, two oppositely charged amino acids, an arginine position 282 aspartate 341 sequence, have been hypothesised to form barrier absorption pathway. In paper we show that appropriate mutations these acids change properties way confirms parts indeed act as electrostatic gate...
The role of internal substrates in the biophysical properties GABA transporter GAT1 has been investigated electrophysiologically Xenopus oocytes heterologously expressing cotransporter. Increments Cl − and/or Na + concentrations caused by intracellular injections did not produce significant effects on pre-steady-state currents, while a positive shift charge-voltage ( Q–V) and decay time constant (τ)-voltage (τ- V) curves, together with slowing τ at potentials, was observed following...
The substrate specificity of KAAT1, a Na+- and K+-dependent neutral amino acid cotransporter cloned from the larva invertebrate Manduca sexta belonging to SLC6A gene family has been investigated using electrophysiological radiotracer methods. KAAT1 was compared that CAATCH1, strictly related transporter with different selectivity. Competition experiments between substrates indicate both transporters bind leucine more strongly than threonine proline, difference CAATCH1 residing in incapacity...