A5055292298- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Neuroscience and Neuropharmacology Research
- Bone Tissue Engineering Materials
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Cancer, Hypoxia, and Metabolism
- Glioma Diagnosis and Treatment
- Dental Implant Techniques and Outcomes
- RNA modifications and cancer
- Wnt/β-catenin signaling in development and cancer
- Estrogen and related hormone effects
- Periodontal Regeneration and Treatments
- Polymer Surface Interaction Studies
- Stress Responses and Cortisol
- Nanoparticle-Based Drug Delivery
- Neural dynamics and brain function
- Corneal Surgery and Treatments
- Eosinophilic Disorders and Syndromes
- Conducting polymers and applications
- Planarian Biology and Electrostimulation
- Corneal surgery and disorders
- Neurogenesis and neuroplasticity mechanisms
The University of Sydney
2023-2025
University of Wollongong
2015-2025
Chris O’Brien Lifehouse
2023-2025
ARC Centre of Excellence for Electromaterials Science
2015-2024
Illawarra Health and Medical Research Institute
2015-2024
The University of Melbourne
2008-2021
St Vincent's Hospital
2021
St Vincents Institute of Medical Research
2011-2015
Agency for Science, Technology and Research
2011
Institute of Molecular and Cell Biology
2009
Direct‐write printing of stem cells within biomaterials presents an opportunity to engineer tissue for in vitro modeling and regenerative medicine. Here, a first example constructing neural by human that are differentiated situ functional neurons supporting neuroglia is reported. The biomaterial incorporates novel clinically relevant polysaccharide‐based bioink comprising alginate, carboxymethyl‐chitosan, agarose. printed rapidly gels stable cross‐linking form porous 3D scaffold...
The ability to create 3D tissues from induced pluripotent stem cells (iPSCs) is poised revolutionize cell research and regenerative medicine, including individualized, patient‐specific cell‐based treatments. There are, however, few examples of tissue engineering using iPSCs. Their culture differentiation predominantly planar for monolayer support or induction self‐organizing embryoids (EBs) organoids. Bioprinting iPSCs with advanced biomaterials promises augment efforts develop tissues,...
OBJECTIVE: Aberrant cholinergic inputs and synaptic neurotransmission in the prefrontal cortex induce cognitive impairment, which is a central feature of schizophrenia. Postsynaptic excitatory muscarinic M1 M4 receptors are major cholinoceptive targets hence may be involved pathology and/or pharmacotherapeutics METHOD: Using quantitative autoradiography, authors analyzed binding M1/M4 receptor selective antagonist [3H]pirenzepine (Brodmann's areas 8, 9, 10, 46) from schizophrenia patients...
Core/shell particles and capsules formed by a layer-by-layer technique are biofunctionalized with humanized A33 monoclonal antibody (huA33 mAb) that binds to the antigen present on colorectal cancer cells (see figure). Targeting cell line shows selective binding internalization of particles. Supporting information for this article is available WWW under http://www.wiley-vch.de/contents/jc_2089/2006/c0564_s.pdf or from author. Please note: The publisher not responsible content functionality...
There has been increased interest in the use of polymer capsules formed by layer-by-layer (LbL) technique as therapeutic carriers to cancer cells due their versatility and ease surface modification. We have investigated influence size, properties, cell line, kinetic parameters such dosage (particle concentration) incubation time on specific binding humanized A33 monoclonal antibody (huA33 mAb)-coated LbL particles colorectal cells. HuA33 mAb binds antigen present almost all demonstrated...
Abstract Electricity is important in the physiology and development of human tissues such as embryonic fetal development, tissue regeneration for wound healing. Accordingly, electrical stimulation (ES) increasingly being applied to influence cell behavior function a biomimetic approach vitro culture engineering. Here, application conductive polymer (CP) poly(3,4‐ethylenedioxythiophene)‐polystyrenesulfonate (PEDOT:PSS) pillars described, direct‐write printed an array format, 3D ES maturing...
Oestrogen modulates cognitive function and affective behaviours subserved by the prefrontal cortex (PFC). Identifying localising oestrogen receptor (ER)α, in human PFC will contribute to our understanding of molecular mechanism action this region. Inferences about site brain are derived largely from studies performed nonhuman mammalian species; however, congruence findings across species has not been demonstrated. Furthermore, laminar, cellular, subcellular localisation ERα is debated....
The normal process of epithelial mesenchymal transition (EMT) is subverted by carcinoma cells to facilitate metastatic spread. Cancer rarely undergo a full conversion the phenotype, and instead adopt positions along epithelial-mesenchymal axis, propensity we refer as plasticity (EMP). EMP associated with increased risk metastasis in breast cancer consequent poor prognosis. Drivers towards state malignant include growth factor stimulation or exposure hypoxic conditions.We have examined two...
Chemotherapy often kills a large fraction of cancer cells but leaves behind small population drug-tolerant persister cells. These survive drug treatments through reversible, non-genetic mechanisms and cause tumour recurrence upon cessation therapy. Here, we report tolerance mechanism regulated by the germ-cell-specific H3K4 methyltransferase PRDM9. Through histone proteomic, transcriptomic, lipidomic, ChIP-sequencing studies combined with CRISPR knockout phenotypic screen, identified that...
Abstract Glioblastoma multiforme is the most devastating brain tumor without cure. Although in vitro and vivo research on glioblastoma have demonstrated its complexity, including interactions with cells microenvironment, 3D models resembling those key features allowing to study therapeutic interventions of this aggressive are scarce. Here, a model developed that establishes microenvironment hyaluronic acid‐based hydrogel cross‐linked laminin, both which components brain's extracellular...
<title>Abstract</title> Patient-matched scaffold guided bone regeneration (SGBR) has been poised to revolutionize the management of critical-sized defects due trauma, tumors, infection, and congenital deformities. However, translation hampered by focus on bioresorbable scaffolds where rate degradation needs be precisely predictably matched new formation. Furthermore, resorbable ceramics polymers require augmentation metal plates overcome their mechanical limitations under load. Consequently,...
Deficits in neurite outgrowth, possibly involving dysregulation of risk genes neuregulin-1 (NRG1) and disrupted schizophrenia 1 (DISC1) have been implicated psychiatric disorders including schizophrenia. Electrical stimulation using conductive polymers has shown to stimulate outgrowth differentiating human neural stem cells. This study investigated the use electroactive polymer polypyrrole (Ppy) counter impaired primary pre-frontal cortical (PFC) neurons from NRG1-knock out (NRG1-KO)...
Electrical stimulation is increasingly being used to modulate human cell behaviour for biotechnological research and therapeutics. Electrically conductive polymers (CPs) such as polypyrrole (PPy) are amenable in vitro vivo stimulation, easy synthesise with different counter ions (dopants) augment biocompatibility cell-effects. Extending our earlier work, which showed that CP-mediated electrical promotes neural stem differentiation, here we report using electroactive PPy containing the...
Electrical stimulation (ES) of cellular systems can be utilized for biotechnological applications and electroceuticals (bioelectric medicine). Neural cell especially has a long history in neuroscience research is increasingly applied clinical therapies. Application ES via conventional electrodes requires external connectors power sources, hindering scientific therapeutic applications. Here engineering novel 3D scaffold-free human neural stem constructs with integrated piezoelectric...