A5029526908- 3D Printing in Biomedical Research
- Silk-based biomaterials and applications
- Additive Manufacturing and 3D Printing Technologies
- Electrospun Nanofibers in Biomedical Applications
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- Nanofabrication and Lithography Techniques
- Bone Tissue Engineering Materials
- Biochemical and Structural Characterization
- Liver physiology and pathology
- RNA Interference and Gene Delivery
- Electrohydrodynamics and Fluid Dynamics
- Viral Infectious Diseases and Gene Expression in Insects
- Neuroscience and Neural Engineering
- Wound Healing and Treatments
- Pickering emulsions and particle stabilization
- Glioma Diagnosis and Treatment
- Cell Image Analysis Techniques
- Planarian Biology and Electrostimulation
- Collagen: Extraction and Characterization
- Advanced Sensor and Energy Harvesting Materials
- Polymer Surface Interaction Studies
- Cancer Cells and Metastasis
Universitätsklinikum Würzburg
2023-2025
Bavarian Polymer Institute
2024-2025
University of Würzburg
2022-2024
University of Bayreuth
2022
Abstract In the context of tissue engineering, biofabrication techniques are employed to process cells in hydrogel-based matrices, known as bioinks, into complex 3D structures. The aim is production functional models or even entire organs. regenerative biological tissues adheres a multitude criteria that ultimately determine maturation tissue. These nature, such biomimetic spatial positioning different cell types within physiologically and mechanically suitable matrix, which enables...
Abstract Glioblastoma multiforme is the most devastating brain tumor without cure. Although in vitro and vivo research on glioblastoma have demonstrated its complexity, including interactions with cells microenvironment, 3D models resembling those key features allowing to study therapeutic interventions of this aggressive are scarce. Here, a model developed that establishes microenvironment hyaluronic acid‐based hydrogel cross‐linked laminin, both which components brain's extracellular...
Biofabrication has proved to be a versatile and valuable tool for tissue engineering cancer research in order mimic different tumor microenvironments. In the present study, four cell lines, including two melanoma Mel Im Wei, breast MDA-MB-231 MCF-7, were tested combination with hydrogels. The hydrogels composite ink consisting of alginate, hyaluronic acid gelatine (Alg/HA/Gel), pre-crosslinked oxidized alginate (ADA-GEL), methyl cellulose (Meth-Alg) acrylated (HAA). Rheological analysis...
Tissue engineering enables the production of tissues and organ-like structures as models for drug testing mechanistical studies or functional replacements injured tissues. Available cytocompatible materials are limited in number, suffer from insufficient mechanical properties, cells interacting with them often cause construct shrinkage. As shape is important function, identifying cytocompatible, shrink-resistant a major aim. Here, it shown that hydrogels made interpenetrating networks...
The development of bio-inks capable being 3D-printed into cell-containing bio-fabricates with sufficient shape fidelity is highly demanding. Structural integrity and favorable mechanical properties can be achieved by applying high polymer concentrations in hydrogels. Unfortunately, this often comes at the expense cell performance since cells may become entrapped dense matrix. This drawback addressed incorporating fibers as reinforcing fillers that strengthen overall bio-ink structure provide...
Abstract Triple‐negative breast cancer (TNBC) is the most invasive type of with high risk brain metastasis. To better understand interactions between tumors extracellular matrix (ECM), a 3D cell culture model implemented using thiolated hyaluronic acid (HA‐SH) based hydrogel. The latter used as HA represents major component ECM. Melt‐electrowritten (MEW) scaffolds box‐ and triangular‐shaped polycaprolactone (PCL) micro‐fibers for hydrogel reinforcement are utilized. Two different molecular...
Soft nano- and microfiber-based polymer scaffolds bear enormous potential for their use in cell culture tissue engineering since they mimic natural collagen structures may thus serve as biomimetic adhesive substrates. They have, however, so far been restricted to small-scale production research labs with high batch-to-batch variation. are commonly produced via electrospinning or melt electrowriting delicate nature poses obstacles detachment, storage, transportation. This study focuses on...
Nucleated protein self-assembly of an azido modified spider silk was employed in the preparation nanofibrillar networks with hydrogel-like properties immobilized on coatings same protein. Formation a mild aqueous environment resulted thicknesses between 2 and 60 nm, which were controlled only by concentration. Incorporated groups used to "click" short nucleic acid sequences onto nanofibrils, accessible specific hybridization-based modifications, as proved fluorescently labeled DNA...
Abstract Photolithography combined with surface nucleated protein self‐assembly of azido‐modified spider silk proteins is used to create an arbitrarily shaped, inherently cell repellent micropattern based on nanofibrillar networks. Using “click” chemistry dibenzocyclooctin modified oligonucleotides, the microstructures are functionalized DNA‐aptamers, which selectively bind cancer markers tyrosine kinase 7 or nucleolin. The epitope‐specific interaction aptamer‐modified surfaces tested using...
ABSTRACT The biological and mechanical environment of cells is better mimicked in 3D compared to 2D cell cultures. However, creating accurate culture models particularly for ultra‐soft tissues like brain or spinal cord challenging since the hydrogels that match these properties are mechanically fragile. Therefore, implementing reinforcing structures, such as microfibers, essential provide necessary support. Particularly, fibrous systems interest they offer natural fibrillar structures...
Abstract Self‐assembly of a recombinant spider silk protein into nanofibrillar networks in combination with photolithography is used to produce diversely functionalized micropattern. Amino‐modified substrates coated positive tone photoresist are processed 1 µm deep arbitrarily shaped microwells, at the bottom which proteins covalently coupled deprotected aminated surface. The layer serves seed self‐assembly nanofibrils from same forming immobilized few nanometers thin after stripping...
The successful in vitro culture of functional primary hepatocytes is essential for drug toxicity tests and the modelling liver diseases. This remains difficult due to rapid dedifferentiation during culture. There are many approaches inhibit hepatocyte dedifferentiation, but these oftentimes lack physiological arrangement or sufficient function. Here, a three-dimensional (3D) matrix used platform suitable establishment an advanced model.
<title>Abstract</title> Nano- and micro-fiber-based scaffolds bear enormous potential for their use in cell culture tissue engineering, since they mimic natural collagen structures may thus serve as biomimetic adhesive substrates. They have, however, so far been restricted to small scale production research labs with high batch-to-batch variation. are commonly produced via electrospinning or melt electro-writing delicate nature poses obstacles detachment, storage, transportation. This study...
Treating large-scale skin wounds continues to be a significant therapeutic challenge. Oftentimes, insufficient autologous material is available achieve full coverage. Since biofabrication offers the chance of reproducible and precise production, aim was develop customized 3D bioprinted construct containing immortalized HaCaT keratinocytes ADSC test principal feasibility this approach. Keratinocytes in different hydrogels varying concentrations alginate, fibrin, collagen, gelatin, gelatin...
This study introduces a standardized approach to generating and assembling G-code for biofabrication, ensuring compatibility convergence across diverse machines scales. Using vector-based drawing software, such as Adobe Illustrator, shapes are designed paths converted into modular blocks (subroutines). allows the straightforward design of complex structures, organic shapes, by simply them scale, avoiding need labour-intensive construction. These assembled final script with modified version...
Abstract A key goal of biofabrication is the production 3D tissue models with biomimetic properties. In natural tissues, fibrils—mainly composed collagen—play a critical role in stabilizing and spatially organizing extracellular matrix. To use fibers for reinforcing bioinks printing, fiber fragmentation necessary to prevent nozzle clogging. However, existing methods are often material‐specific, poorly scalable, provide limited control over fragment size shape. novel workflow introduced...