A5079332209- Advanced Fluorescence Microscopy Techniques
- Nitric Oxide and Endothelin Effects
- Protease and Inhibitor Mechanisms
- Cardiac electrophysiology and arrhythmias
- Receptor Mechanisms and Signaling
- Acute Myeloid Leukemia Research
- Asthma and respiratory diseases
- Cellular transport and secretion
- bioluminescence and chemiluminescence research
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Photoreceptor and optogenetics research
- Biotin and Related Studies
- Ion channel regulation and function
- Lipid Membrane Structure and Behavior
- Photosynthetic Processes and Mechanisms
- Venous Thromboembolism Diagnosis and Management
- Neuroscience and Neural Engineering
- Endoplasmic Reticulum Stress and Disease
- Wnt/β-catenin signaling in development and cancer
- Biocrusts and Microbial Ecology
- Advanced Electron Microscopy Techniques and Applications
- Nuclear Structure and Function
- Blood Coagulation and Thrombosis Mechanisms
- Optical Imaging and Spectroscopy Techniques
Monash University
2012-2022
Ludwig Cancer Research
2000-2009
The Royal Melbourne Hospital
2000-2009
Howard Hughes Medical Institute
2003
Deakin University
2003
The University of Melbourne
1990-2001
The University of Western Australia
1997
Core/shell particles and capsules formed by a layer-by-layer technique are biofunctionalized with humanized A33 monoclonal antibody (huA33 mAb) that binds to the antigen present on colorectal cancer cells (see figure). Targeting cell line shows selective binding internalization of particles. Supporting information for this article is available WWW under http://www.wiley-vch.de/contents/jc_2089/2006/c0564_s.pdf or from author. Please note: The publisher not responsible content functionality...
Laser scanning confocal microscopy of the Ca(2+)-sensitive fluorophore fluo-3 has been used to investigate spontaneous and propagated calcium release at high temporal spatial resolution in enzymatically dispersed rat cardiomyocytes. Waves fluorescence which throughout cytosol were evident spontaneously contracting cardiac cells containing fluo-3, but not Ca(2+)-insensitive fluorophores [2',7'-bis (carboxyethyl)-5,6-carboxyfluorescein, SNARF-1, rhodamine-123, or tetramethylrhodamine-labeled...
The murine A33 antigen is emerging as a definitive marker of intestinal epithelial cells. Cloning and sequence determination cDNAs encoding mA33 predict novel type 1 transmembrane protein 298 amino acids, comprising an extracellular domain with two immunoglobulin-like domains, single-span domain, highly acidic cytoplasmic domain. On the basis conservation acid genomic structure, member growing subfamily within immunoglobulin superfamily, which includes proteins CTX/ChT1, CTM/CTH, CAR. During...
Cellular injury causes a myriad of processes that affect proteostasis. We describe nucleocytoplasmic coagulation (NCC), an intracellular disulfide-dependent protein crosslinking event occurring upon late-stage cell death orchestrates the proteolytic removal misfolded proteins. In vitro and in vivo models neuronal show NCC involves conversion soluble proteins, including tubulin, into insoluble oligomers. These oligomers, also seen human brain tissue following neurotrauma, act as cofactor...
Nanomedicine holds great promise for vascular disease diagnosis and specific therapy, yet rapid sequestration by the mononuclear phagocytic system limits efficacy of particle-based agents. The use low-fouling polymers, such as poly(ethylene glycol), efficiently reduces this immune recognition, but these nondegradable polymers can accumulate in human body may cause adverse effects after prolonged use. Thus, new particle formulations combining stealth, low immunogenicity biocompatible features...
We describe the first direct visualization of Ca2+ oscillations in perfused whole rat heart. Dye loading at a low temperature and enhanced optical-section techniques confocal microscopy by elimination refractive index mismatch with use saline-immersible objective lens enabled us to image multiple waves subepicardial myocardium fluo 3-loaded These were sporadically seen even physiological extracellular perfusion either paced or an arrested heart propagated beyond cellular boundaries within...
AIBP (apolipoprotein A-I binding protein) is an effective and selective regulator of lipid rafts modulating many metabolic pathways originating from the rafts, including inflammation. The mechanism action was suggested to involve stimulation by cholesterol efflux, depleting cholesterol, which essential for raft integrity. Here we describe a different contributing regulation AIBP. Approach Results: We demonstrate that modulation may not exclusively depend on rate efflux or presence key ABCA1...
We report the development of a theranostic collagen targeted cell penetrating drug delivery system toward treatment cardiovascular disease. Caused by action matrix metalloproteinases (MMP), degraded is hallmark unstable atherosclerotic plaques that are highly susceptible rupture. Targeting and delivering MMP blockers directly to plaque inhibit activity promising new strategy requires benefits possibilities nanodelivery approaches. The presented designed (a) target bind cryptic epitope on IV...
Arterial thrombosis models are important for preclinical evaluation of antithrombotics but how anesthetic protocol can influence experimental results is not studied.We studied three most commonly used rodent anesthetics affect the induction and thrombus resolution with antiplatelet agent integrilin (Eptifibatide).The Folts, electrolytic, FeCl3 carotid artery were evaluated. The extent blood flow reduction required to elicit cyclic reductions (CFR) was examined in Folts model. occlusion time...
Summary Current optical methods to collect Nomarski differential interference contrast (DIC) or phase images with a transmitted light detector (TLD) in conjunction confocal laser scanning microscopy (CLSM) can be technically challenging and inefficient. We describe for the first time simple method that combines use of commercial product QPm (Iatia, Melbourne Australia) brightfield collected TLD CLSM, generating DIC, phase, Zernike dark‐field Hoffman modulation images. The may at same as This...
Endothelin-1(ET-1) has been shown to potentiate cholinergic neurotransmission in human bronchus as well airways from a variety of animal species, suggesting that ET receptors exist prejunctionally on airway nerves. We have successfully isolated and maintained rat tracheal parasympathetic neurons culture. Most cultured cells were associated with specific fluorescence for the nerve cell marker protein gene product 9.5 (PGP 9.5). These cultures contained high proportion neurons. Importantly,...
Abstract Vascular injury models are indispensable for studying thrombotic processes in vivo. Amongst the available methods inducing thrombosis, laser-induced endothelial (LIEI) has several unique advantages. However, a lack of methodological standardization and expensive instrumentation remain significant problems decreasing reproducibility impeding adoption LIEI wider scientific community. In this, study, we developed standardized protocol scanning (scanning-LIEI) murine mesenteric veins...