A5012918166- Cardiovascular Function and Risk Factors
- Cardiac electrophysiology and arrhythmias
- Cardiomyopathy and Myosin Studies
- Autophagy in Disease and Therapy
- Cardiac Ischemia and Reperfusion
- Hormonal Regulation and Hypertension
- Cardiac Fibrosis and Remodeling
- Pancreatic function and diabetes
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Cardiovascular Disease and Adiposity
- Renin-Angiotensin System Studies
- Metabolism, Diabetes, and Cancer
- Diet, Metabolism, and Disease
- Cardiovascular Effects of Exercise
- Liver Disease Diagnosis and Treatment
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Congenital heart defects research
- Digital Holography and Microscopy
- Estrogen and related hormone effects
- ATP Synthase and ATPases Research
- Cardiac Imaging and Diagnostics
- Birth, Development, and Health
The University of Melbourne
2015-2024
University of Auckland
2016-2024
Ridge Lea Hospital
2014-2019
O'Brien Institute
2017
St Vincents Institute of Medical Research
2017
Hudson Institute of Medical Research
2015
Monash University
2007-2012
Sorbonne Paris Cité
2012
Université Paris Cité
2012
Federation University
2012
Abstract Background The refractive index (RI) of cellular material provides fundamental biophysical information about the composition and organizational structure cells. Efforts to describe properties cells have been significantly impeded by experimental difficulties encountered in measuring viable cell RI. In this report we a procedure for application quantitative phase microscopy conjunction with confocal measure RI cultured muscle specimen. Methods strategy involved calculation thickness...
The objectives of this study were to measure action potential parameters in enzyme-dissociated, adult rat ventricular myocytes stimulated at 1 Hz, compare these measurements with those obtained from intact tissue, and determine myocyte tissue responses stimulus frequencies between 0.1 5 Hz. Action potentials characterized terms amplitude, overshoot, resting potential, duration 25% 75% repolarization (APD25, APD75), Vmax. Based on statistical differences APD25 APD75, classified as type I (3.1...
Mineralocorticoid receptor (MR) activation promotes the development of cardiac fibrosis and heart failure. Clinical evidence demonstrates that MR antagonism is protective even when plasma aldosterone levels are not increased. We hypothesize in macrophages drives profibrotic phenotype elevated. The aim present study was to establish role macrophage signaling mediating tissue remodeling caused by nitric oxide (NO) deficiency, a mineralocorticoid-independent insult. Male wild-type (MRflox/flox)...
Congenital heart defects can be caused by mutations in genes that guide cardiac lineage formation. Here, we show deletion of NKX2-5, a critical component the gene regulatory network, human embryonic stem cells (hESCs), results impaired cardiomyogenesis, failure to activate VCAM1 and downregulate progenitor marker PDGFRα. Furthermore, NKX2-5 null cardiomyocytes have abnormal physiology, with asynchronous contractions altered action potentials. Molecular profiling genetic rescue experiments...
Male sex, early life chemical exposure and the brain aromatase enzyme have been implicated in autism spectrum disorder (ASD). In Barwon Infant Study birth cohort (n = 1074), higher prenatal maternal bisphenol A (BPA) levels are associated with ASD symptoms at age 2 diagnosis 9 only males low genetic pathway activity scores. Higher BPA predictive of cord blood methylation across CYP19A1 promoter I.f region (P 0.009) gene mediates 0.01) link between brain-derived neurotrophic factor...
Diabetic heart disease morbidity and mortality is escalating. No specific therapeutics exist mechanistic understanding of diabetic cardiomyopathy etiology lacking. While lipid accumulation a recognized cardiomyocyte phenotype diabetes, less known about glycolytic fuel handling storage. Based on in vitro studies, we postulated the operation an autophagy pathway myocardium for glycogen homeostasis - glycophagy. Here visualize occurrence cardiac glycophagy show that characterized by marked...
Autophagy has emerged as an important process in the pathogenesis of cardiovascular diseases, but proximal triggers for autophagy are unknown. Angiotensin II plays a central role cardiac hypertrophy and heart failure. In this study, we used angiotensin type 1 (AT ) 2 receptor–expressing adenoviruses cultured neonatal cardiomyocytes to provide first demonstration that cardiomyocyte autophagic activity is differentially modulated by AT receptor subtypes. stimulation (48 hours) expressing alone...
Because the role of mineralocorticoid receptors in specific cell types cardiac remodeling remains unknown, we have compared responses with deoxycorticosterone/salt cardiomyocyte receptor-null (MyoMRKO) and wild-type (WT) mice at 8 days weeks. No differences function between untreated WT MyoMRKO were found, whereas profibrotic markers reduced hearts baseline. At days, showed monocyte/macrophage recruitment equivalent to response but a suppression fibrosis WT. weeks, no...
Cardiac metabolic stress is a hallmark of many cardiac pathologies, including diabetes. glycogen mis-handling frequent manifestation various cardiopathologies. Diabetic females have higher risk heart disease than males, yet sex disparities in settings are not well understood. Oestrogen acts on key regulatory proteins. The goal this study was to evaluate sex-specific stress-triggered handling responses. Male and female adult C57Bl/6J mice were fasted for 48 h. content, particle size, enzymes,...
Background Cardiac hypertrophy increases the risk of developing heart failure and cardiovascular death. The neutrophil inflammatory protein, lipocalin‐2 ( LCN 2/ NGAL ), is elevated in certain forms cardiac acute failure. However, a specific role for 2 predisposition etiology relevant genetic determinants are unclear. Here, we defined concentric terms pathophysiology, expression networks, genomic determinants. Methods Results We used 3 experimental models: polygenic model failure,...
Chronic elevation of plasma angiotensin II (Ang II) is detrimental to the heart. In addition its hemodynamic effects, Ang exerts cardiotrophic actions that contribute cardiomyocyte remodeling. However, it remains be clarified whether these direct are sufficient cause contractile dysfunction and heart failure in absence altered conditions. this study, we used TG1306/1R (TG) mice develop II–mediated cardiac hypertrophy elevated blood pressure investigate phenotypic changes cardiomyocytes...