Osteosarcoma is the predominant form of primary bone malignancy. Although combinational application neoadjuvant chemotherapy and surgical resection significantly increases survival rate, therapeutic outcome remains unsatisfactory. Deoxyelephantopin (DET), an active ingredient Elephantopus scaber, has been reported to have anti-tumor effect in recent publications. This study aimed investigate whether DET antineoplastic effects on osteosarcoma cells its underlying mechanism.Cell viability...

Osteosarcoma (OS) is a type of malignant primary bone cancer, which highly aggressive and occurs more commonly in children adolescents. Thus, novel potential drugs therapeutic methods are urgently needed. In the present study, we aimed to elucidate effects mechanism melatonin on OS cells provide treatment strategy for OS. The cell survival rate, viability, proliferation, migration, invasion metastasis were examined by trypan blue assay, MTT, colony formation, wound healing, transwell...

Abstract There are multiple published data showing that excessive oxidative stress contributes to bone loss and even tissue damage, it is also correlated with the pathophysiology of degenerative diseases, including osteoporosis (OP). Garcinol, a polyisoprenylated benzophenone derivative, has been recently established as an anti-oxidant agent. However, remains elusive whether Garcinol protects marrow mesenchymal stem cells (BMSCs) from stress-induced damage. Here, we explored potential...

Previous studies have demonstrated that extracellular matrix (ECM) can be used in tissue engineering due to its bioactivity. However, adipose-derived ECM (A-dECM) has never been applied bone engineering, and it is unknown whether would beneficial the growth of marrow mesenchymal stem cells (BMSCs). In this study, we produced chitosan/gelatin/A-dECM (C/G/A-dECM) scaffolds via lyophilization crosslinking; chitosan/gelatin (C/G) were as controls. For C/G/A-dECM scaffolds, average pore size was...

Osteosarcoma (OS) is the most common primary malignant tumor of bone tissue. Although treatment effectiveness has improved, OS survival rate fluctuated in recent years. Andrographolide (AG) been reported to have antitumor activity against a variety tumors. Our aim was investigate effects and potential mechanisms AG human osteosarcoma.Cell viability morphological changes were assessed by MTT live/dead assays. Apoptosis detected using Annexin V-FITC/PI double staining, DAPI, caspase-3...

In this study, we evaluated the expressions and functions of a long noncoding RNA (lncRNA), Noncoding activated by DNA damage (NORAD) in human osteosarcoma. NORAD were qRT-PCR vitro osteosarcoma cell lines vivo clinical specimens. SiRNA-induced downregulation was conducted Saos-2 143B cells, functional effects on cells CCK-8 proliferation assay, 24-well transwell invasion assay tumor explant respectively. The possibility endogenously competing microRNA target, hsa-miR-199a-3p examined...

More and more studies have confirmed that miRNAs play an important role in maintaining bone remodeling metabolism. This study investigated the expression level of miR-206 serum osteoporosis (OP) patients explored effect mechanism on occurrence development osteoporosis.120 postmenopausal women were recruited, including 63 cases with OP 57 without OP. The levels determined by qRT-PCR technology. Spearman correlation coefficient was used to evaluate mineral density (BMD). An ROC curve...

Human osteosarcoma is considered a malignant tumor with poor prognosis that readily metastasizes. Tetrahydrocurcumin (THC) has been reported to have anti-tumor activity in numerous tumors. In addition, hypoxia-inducible factor-1α (HIF-1α) demonstrated be associated metastasis by regulating epithelial-mesenchymal transition (EMT). However, the role of THC remains uncertain. Therefore, this study aimed elucidate potential mechanisms. We found significantly reduced growth cells and suppressed...

Bone marrow mesenchymal stem cells (BMSCs) are a potential source of osteoblasts and have been widely used in clinical therapies due to their pluripotency. Recent publications found that resveratrol (RSVL) played crucial role the proliferation differentiation BMSCs; however, underlying molecular mechanism RSVL-induced BMSCs osteogenic needs be fully elucidated. The objective this study was explore functions miRNAs RSVL-treated its effects on potentials BMSCs. findings demonstrated RSVL...

MicroRNAs (miRNAs) play a vital role in the bone development and regeneration. In this study, we investigated effects of miR-26a osteoblasts fractures.Human were cultured used for analysis. To identify differential miRNAs blood samples from patients with fractures healthy controls, quantitative real-time polymerase chain reaction (qRT-PCR) analysis was performed. Human transfected mimics, inhibitor, or their corresponding negative controls (NCs), respectively. MTT assay performed to on cell...

Background: The proteasome exists in all eukaryotic cells and provides the main route of intracellular proteins degradation involved cell growth apoptosis. Proteasome inhibition could block protein pathways disturb regulatory networks, possibly leading to profound effects on growth, particularly cancer cells. A inhibitor with an appropriate toxicity index for malignant rather than normal would be attractive anticancer therapy. Methods: human osteosarcoma (OS) lines MG-63 Saos-2 osteoblast...

The activation of oxidative stress is a primary cause chondrocyte apoptosis in osteoarthritis (OA). 78‑kDa glucose‑regulated protein (GRP78)/mammalian target rapamycin (mTOR) signaling pathway has been demonstrated to be linked with the endoplasmic reticulum (ER) and autophagy. Hydrogen sulfide (H₂S) reported exert antioxidant effects. present study investigated levels via 2',7'‑dichlorofluorescin diacetate MitoSOX staining, rates flow cytometry expression ER stress‑related...

Caffeine is broadly present in tea, coffee, and cocoa, commonly consumed. The bone microenvironment might be damaged by excessive caffeine, which has been shown to exert negative effects on human health. In this study, we sought determine whether caffeine could damage the biological functions of marrow mesenchymal stem cells (BMSCs) induce loss mice, further investigate effective therapeutic methods.BMSCs were treated with different concentrations (0.01, 0.05, 0.1, 0.5, 1.0 mM) for 48 h....

Osteosarcoma is an aggressive cancer of the skeletal system, which associated with a poor prognosis due to high recurrence rate. Although previous studies have revealed that competitive endogenous RNAs (ceRNAs) are involved in various biological processes physiology and development osteosarcoma, roles ceRNAs osteosarcoma remain largely unexplored. The present study constructed ceRNA‑ceRNA network for by systematically integrating matched expression profiles microRNAs (miRNAs/miRs) mRNAs,...

// Xiaoyan Wang 1, 2 , Guanghua Chen Chao Huang 1 Hualei Tu 3 Jilong Zou 4 and Jinglong Yan Department of Orthopedics, The Second Affiliated Hospital Harbin Medical University, 150086, P.R. China Key Laboratory the Ministry Education, Myocardial Ischemia, Burn Department, Fifth Harbin, First 150001, Correspondence to: Yan, email: yanjinglong246@163.com Keywords: extracellular matrix, BMSCs, tissue engineering, scaffold, bone regeneration Received: August 24, 2017 Accepted: September 20,...

Previous articles reported on the tip-apex distance, lag screw placement, fracture pattern, reduction quality, osteoporosis and other factors associated with second surgery. The current study focused investigating association of matching degree between proximal femoral intramedullary nail medullary cavity reoperation rate.A retrospective cohort was conducted. It included patients intertrochanteric who were treated anti-rotatory (PFNA) January 2016 April 2021. gap equal to difference in...

Summary The blowout preventer (BOP) stack is the last line of defense for well control (pressure wellbore), and failure its sealing components will cause serious accidents. At present, there a lack suitable experimental device to verify feasibility emergency rescue method proposed early seal BOP by pumping plugging particles. However, it costly, risky, time-consuming build implement large-scale model with ultrahigh pressure (70 MPa) large displacement. Therefore, based on this device, paper...

The medical damage litigations after knee arthroplasty are on the rise year by year. However, few studies examined litigation arthroplasty. This study analyzed of replacement in past ten years based a Chinese database. It synthesized focus dispute these cases to provide reference for doctors reduce risk litigation.