Stem cell therapy has emerged as a potential modality for myocardial infarction treatment. Mesenchymal stem cells (MSCs) exert reparative actions in the injured myocardium mainly through secretion of paracrine factors. In addition, overexpression connexin 43 (Cx43), gap junction protein, promotes cardiac repair and function restoration. It is known that MSCs spheroid form, which have enhanced cell–cell interaction, exhibit expression factors Cx43. However, cell‐extracellular matrix (ECM)...

Pressure overload in the heart induces pathological hypertrophy and is associated with cardiac dysfunction. Apoptosis fibrosis signaling initiated by endoplasmic reticulum stress (ERS) known to contribute these maladaptive effects. The aim of this study was investigate whether reduction ERS a chemical chaperone, tauroursodeoxycholic acid (TUDCA) can attenuate pressure overload-induced remodeling mouse model transverse aortic constriction (TAC). Oral administration TUDCA at dose 300 mg/kg...

Abstract We examined whether a shift in macrophage phenotype could be therapeutic for myocardial infarction ( MI ). The mouse cell line RAW 264.7 was stimulated with peptidoglycan PGN ), or without 5‐azacytidine (5 AZ ) treatment. induced by ligation of the left anterior descending coronary artery rats, and rats were divided into two groups; saline‐injection group 5 ‐injection (2.5 mg/kg/day, intraperitoneal injection). LV function evaluated immunohistochemical analyses performed 2 weeks...

Abstract Type 2 diabetes mellitus (T2DM) significantly impacts on human health and patient numbers are predicted to rise. Discovering novel drugs targets for treating T2DM is a research priority. In this study, we investigated targeting of the glycolysis enzyme, enolase, using small molecule ENOblock, which binds enolase modulates its non-glycolytic ‘moonlighting’ functions. insulin-responsive cells ENOblock induced nuclear translocation, where enzyme acts as transcriptional repressor....

The extraction of sulforaphane (SFN) is challenging due to its instability and low water solubility, with existing methods often involving toxic solvents or yielding SFN. We optimized an endogenous protocol for high SFN content, characterized by HPLC LC-MS analyses. remained stable in refrigerated broccoli sprout extract powder (BSEP) over a month. BSEP showed four times higher oxygen radical absorbance capacity (ORAC) than the standard, indicating antioxidant capacity. It also reduced...

Abstract Macrophages are actively involved in inflammatory responses during the progression of cardiac injury, including myocardial infarction (MI). A previous study showed that 5-azacytidine (5AZ), a DNA methylation inhibitor, can ameliorate injury by shifting macrophages toward an anti-inflammatory phenotype via iNOS inhibition. Here, we show beneficial effect 5AZ is associated with sumoylation interferon regulatory factor-1 (IRF1) macrophages. IRF1 critical transcription factor for...

Abstract The cardiac microenvironment includes cardiomyocytes, fibroblasts and macrophages, which regulate remodeling after myocardial infarction (MI). Targeting this is a novel therapeutic approach for MI. We found that the natural compound derivative, BIO ((2′Z,3′E)-6-Bromoindirubin-3′-oxime) modulated to exert effect on Using series of co-culture studies, induced proliferation in cardiomyocytes inhibited fibroblasts. produced multiple anti-fibrotic effects In expression pro-inflammatory...

Abstract Background Angiogenesis is the main therapeutic mechanism of cell therapy for cardiovascular diseases, but diabetes reported to reduce function and number progenitor cells. Therefore, we studied effect streptozotocin-induced on bone marrow-mesenchymal stem (MSC) function, examined whether diabetes-impaired MSC could be rescued by pretreatment with oxytocin. Results MSCs were isolated cultured from diabetic (DM) or non-diabetic (non-DM) rat, proliferation rate was compared. DM-MSC...

Our previous study demonstrated that a 5% ethanol extract of unripe Rubus coreanus (5-uRCK) has hypo-cholesterolemic and anti-obesity activity. However, the molecular mechanisms its effects are poorly characterized. We hypothesized 5-uRCK one major bioactive compounds, ellagic acid, decrease cellular plasma cholesterol levels. Thus, we investigated hypocholesterolemic activity mechanism in both hepatocytes high-cholesterol diet (HCD)-induced rat model. Cholesterol liver serum was...

This study identified microRNAs involved in myocardial infarction (MI) through a novel system-level approach using RNA sequencing data an MI mouse model. the extraction of DEGs and DEmiRs from RNA-seq sham samples subsequent selection two miRNAs: miR-30-5p (family) miR-142a-5p, which were downregulated upregulated MI, respectively. Gene Set Enrichment Analysis (GSEA) predicted targets miRNAs suggested that apoptosis is essential gene ontology (GO)-associated term. In vitro functional assays...

We investigated the time-dependent deleterious ocular changes induced by urban particulate matter (UPM) in vitro and vivo. UPM treatment decreased human corneal epithelial cell migration survival. Fluorescein scores were consistently increased application for 16 weeks. One week of rest at 2 or 4 weeks led to a recovery trend, whereas two 8 no change. tear film break-up time weeks, which was thereafter maintained until No found after periods rest. UPM-treated eyes exhibited greater epithelium...

Foam cell formation from macrophage is a major cause of atherosclerosis. An efficient macrophage-specific promoter required for the targeting to macrophages. In this study, we develop synthetic therapeutic application adiponectin (APN), an antiatherogenic gene. Synthetic promoter-146 (SP146), registered on NCBI website ( http://www.ncbi.nlm.nih.gov/nuccore/DQ107383 ), was tested activities in two non-macrophage lines (293 T, HeLa) and line (RAW264.7, bone marrow-derived macrophages). To...

Dry eye disease is affected by a broad range of causes such as age, lifestyle, environment, medication and autoimmune diseases. These induce tear instability that activates immune cells promotes expression inflammatory molecules. In this study, we investigated the therapeutic effects an ethanolic extract Aucuba japonica (AJE) its bioactive compound, aucubin, on dry disease. The human corneal were exposed to desiccation stress induced exposing air, so viability was decreased. On other hand,...

Blue light is reported to be harmful eyes by inducing reactive oxygen species (ROS). Herein, the roles of Peucedanum japonicum Thunb. leaf extract (PJE) in corneal wound healing under blue irradiation are investigated. Blue-light-irradiated human epithelial cells (HCECs) show increased intracellular ROS levels and delayed without a change survival, these effects reversed PJE treatment. In acute toxicity tests, single oral administration (5000 mg/kg) does not induce any signs clinical or body...

// Dong Im Cho 1 , Wan Seok Kang Moon Hwa Hong Hye Jin Mi Ra Kim Min Chul 2 Yong Sook 1, 3 and Youngkeun Ahn Cell Regeneration Research Center, Chonnam National University Hospital, Gwangju, South Korea Department of Cardiology, Biomedical Institute, Correspondence to: Ahn, email: cecilyk@hanmail.net Kim, retissue@jnu.ac.kr Keywords: mesenchymal stem cell, myocardial infarction, apicidin, differentiation, cotransplantation Received: March 13, 2017     Accepted: April 16,...

Background and Objectives: Diabetes is reported to reduce the function or number of progenitor cells.We compared gene expression patterns bone marrow-derived mesenchymal stem cells from diabetic (DM-BMCs) healthy (non-DM-BMCs) rats suggested Angiopoietin-like 4 (Angptl4) could be a responsible factor for impaired angiogenesis DM-BMCs.Subjects Methods: BMCs were isolated DM non-DM rat, in vitro activity was by tube formation assay on Matrigel complementary deoxyribonucleic acid analyzed...

The renal accumulation of advanced glycation end products (AGEs) is a causative factor various diseases, including chronic kidney disease and diabetic nephropathy. AGE inhibitors, such as aminoguanidine pyridoxamine, have the therapeutic activities for reversing increase in burden. This study evaluated inhibitory effects ethyl pyruvate (EP) on methylglyoxal- (MGO-) modified cross-links with proteins vitro. We also determined potential activity EP reducing burden exogenously MGO-injected...

Intramyocardial Injection of Stem Cells in Pig Myocardial Infarction Model: The First Trial KoreaAlthough cell therapy is emerged for cardiac repair, its efficacy modest by intracoronary infusion.Therefore, we established the intramyocardial delivery technique using a left ventricular (LV) mapping system (NOGA® XP) 18 pigs.After adipose tissue-derived mesenchymal stem cells (ATSCs) were delivered intramyocardially to porcine infarcted heart, LV ejection fraction (EF) was increased, and...

Patients with diabetes commonly experience hyposalivation, which induces discomfort in eating, swallowing, dryness, smell, and speaking, as well increases the incidence of periodontal disease. Dipeptidyl peptidase-4 (DPP4) inhibitors are frequently used antidiabetic drugs that lower glucose levels by utilizing similar mechanisms; however, additional protective functions each gliptin have been discovered. In this study, roles gemigliptin, a DPP4 inhibitor, against salivary dysfunction under...

We previously demonstrated that urban particulate matter (UPM) exposure decreases the migration activity and survival of human corneal epithelial cells (HCECs). Herein, we investigated potential to improve wound-healing ability

Recently, many studies have reported the properties and functionality of okra pectin. However, these are about green pods, pectin in red stems, roots has not been reported. Therefore, this study aimed to optimize extraction method using response surface methodology (RSM) analysis evaluate effects time, temperature, solvent ratio, pH on extract yield crude content. Based RSM analysis, 4.35 h, 98.04 °C, 23.34 3.36 optimal parameters for extracting from okra, content was predicted be 40.83%....

Abstract Background Many clinical trials on antihypertensive drugs have confirmed the usefulness of these in regulating blood pressure effectively. However, all usually require long-term use; thus, economic burdens as well some adverse effects, including headache, diarrhea, skin rash, edema, fever, and liver kidney dysfunction, accompany their use. Therefore, we attempted to identify natural medications for treating hypertension. We investigated effects Dendropanax morbiferus H. Lév. extract...