A5100747802- Microbial Metabolic Engineering and Bioproduction
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Genetics and Neurodevelopmental Disorders
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- DNA Repair Mechanisms
- Genomics and Phylogenetic Studies
- Congenital heart defects research
- Cancer Genomics and Diagnostics
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Bioinformatics and Genomic Networks
- Gastric Cancer Management and Outcomes
- Cardiac Fibrosis and Remodeling
- Cancer-related Molecular Pathways
- Tissue Engineering and Regenerative Medicine
- HER2/EGFR in Cancer Research
- Gut microbiota and health
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Fungal and yeast genetics research
- Computational Drug Discovery Methods
Seoul National University Hospital
2004-2024
Pusan National University
2021-2024
Memorial Sloan Kettering Cancer Center
2023
Kangbuk Samsung Hospital
2022
San Diego State University
2017-2021
University of California, Berkeley
2021
Dong-Eui University
2021
Moores Cancer Center
2020
Regenerative Medicine Institute
2019
Sungkyunkwan University
2019
Cell-based therapies represent a very promising strategy to repair and regenerate the injured heart prevent progression failure. To date, these have had limited success due lack of survival retention infused cells. Therefore, it is important increase our understanding biology cells utilize this information enhance their function in heart. Mitochondria are critical for progenitor cell survival. Here, we demonstrate importance mitochondrial autophagy, or mitophagy, differentiation process...
Although both physiological hypertrophy (PHH) and pathological (PAH) of the heart have similar morphological appearances, only PAH leads to fatal failure. In present study, we used RNA sequencing (RNA-Seq) determine transcriptomic signatures for PHH PAH. Approximately 13-20 million reads were obtained models, among which showed more differentially expressed genes (DEGs) (2,041) than (245). The expression 417 was barely detectable in normal but suddenly activated Among them, Foxm1 Plk1 are...
Microbiota plays a critical role in the overall growth performance and health status of dairy cows, especially during their early life. Several studies have reported that fecal microbiome neonatal calves is shifted by various factors such as diarrhea, antibiotic treatment, or environmental changes. Despite importance gut microbiome, lack knowledge regarding composition functions microbiota impedes development new strategies for improving disease resistance calf period. In this study, we...
This study aimed to develop a novel multiplex polymerase chain reaction (PCR) primer set for the identification of seven probiotic Lactobacillus species such as acidophilus, delbrueckii, casei, gasseri, plantarum, reuteri and rhamnosus. The set, comprising specific two conserved primers, was derived from integrated sequences 16S 23S rRNA genes their intergenic spacer region each species. It able identify target with 93.6% accuracy, which exceeds that general biochemical methods. phylogenetic...
Abstract How cell fate (survival or death) is determined and whether such determination depends on the strength of stimulation has remained unclear. In this study, we discover that cardiomyocytes switches from survival to death with increase β-adrenergic receptor (β-AR) stimulation. Mathematical simulations combined biochemical experimentation β-AR signalling pathways show gradual increment isoproterenol (a non-selective β 1 /β 2 -AR agonist) induces switching response Bcl-2 expression...
A causal relationship between diabetes mellitus (DM) and pancreatic cancer is well established. However, in patients with advanced (APC) who receive palliative chemotherapy, the impact of DM on prognosis APC unclear.We retrospectively enrolled received chemotherapy 2003 2010. The were stratified according to status DM, accordance 2010 criteria (American Heart Association/American Diabetes Association). at least 2 years' duration prior diagnosis was defined as remote-onset (vs....
The relative actions and synergism between distinct myocardial-derived stem cell populations remain obscure. Ongoing debates on optimal population(s) for treatment of heart failure prompted implementation a protocol isolation multiple from single myocardial tissue sample to develop new insights achieving regeneration.Establish robust cardiac culture consistently generate 3 human biopsy.Isolation endogenous was performed samples routinely discarded during implantation left ventricular assist...
Senescence-associated dysfunction deleteriously affects biological activities of human c-Kit+ cardiac progenitor cells (hCPCs), particularly under conditions in vitro culture. In comparison, preservation self-renewal and decreases mitochondrial reactive oxygen species (ROS) are characteristics murine CPCs vivo that reside within hypoxic niches. Recapitulating niche tension ∼1% O2 for expansion hCPCs rather than typical normoxic cell culture (21% ) could provide significant improvement...
Abstract Background Claudin 18.2 (CLDN18.2) is a promising target for targeted therapies in gastric cancer (GC). This study investigated the prevalence of CLDN18.2 expression patients with stages II-IV GC or gastroesophageal junction (GEJ) adenocarcinoma and its correlation clinicopathologic features other crucial biomarkers. Methods We enrolled 1000 diagnosed after surgical treatment. Immunohistochemistry CLDN18 (43-14A clone), PD-L1 (22C3 pharmDx), HER2, FGFR2 was performed. positivity...
The association between the CpG island methylator phenotype (CIMP) and clinical outcomes in metastatic colorectal cancer remains unclear. We investigated prognostic impact of CIMP patients with treated systemic chemotherapy. Eight CIMP-specific promoters (CACNA1G, IGF2, NEUROG1, RUNX3, SOCS1, CDKN2A, CRABP1, MLH1) were examined. status was determined by number methylated as high (⩾5), low (1–4), negative (0). A total 153 included (men/women, 103/50; median age, 61 years; range, 22–80 years)....
Abstract Background: Polo-like kinase 1 (PLK1) has been emerging as a promising therapeutic target in solid tumors due to its central role cell cycle regulation and cancer proliferation. In biliary tract (BTC), cycle-related factors such PLK1, CDKs, Aurora are frequently overexpressed or aberrantly activated, driving tumor progression. However, studies targeting these proteins BTC remain limited. BUBR1, critical mitotic checkpoint protein, is essential for maintaining chromosomal stability...
AMPA-type glutamate receptors play a key role in mediating postsynaptic responses of excitatory neurotransmitters. It is now well accepted that AMPA are also present at the presynapse, where they thought to modulate neurotransmitter release. However, mechanisms through which control synaptic vesicle traffic have remained elusive. We used cultured hippocampal neurons and growth cone particles prepared from fetal rat brain investigate functional presynaptic receptors. show here stimulation...
Calumenin is a multiple EF-hand Ca(2+)-binding protein localized in the sarcoplasmic reticulum (SR) with C-terminal SR retention signal HDEF. Recently, we showed evidence that calumenin interacts SERCA2 rat cardiac (Sahoo, S. K., and Kim, D. H. (2008) Mol. Cells 26, 265-269). The present study was undertaken to further characterize association of mouse heart by various gene manipulation approaches. Immunocytochemical analysis were partially co-localized HL-1 cells. Knockdown (KD) conducted...
Graphene has attracted substantial attention due to its advantageous materialistic applicability. In the present study, we tested biocompatibility of graphene films synthesized by chemical vapor deposition with electrogenic primary adult cardiac cells (cardiomyocytes) measuring cell properties such as attachment, survival, contractility and calcium transients. The results show that showed stable attachment excellent cardiomyocytes, suggesting their useful applications for future biology studies.
Regenerative therapeutic approaches for myocardial diseases often involve delivery of stem cells expanded ex vivo. Prior studies indicate that cell culture conditions affect functional and phenotypic characteristics, but relationship(s) cultured derived from freshly isolated populations the heterogeneity population remain poorly defined. Functional characteristics vivo will determine outcomes interventional treatment disease, necessitating characterization impact expansion has upon...
Germline mutations of breast cancer susceptibility gene BRCA1 and BRCA2 (gBRCA1/2) are associated with elevated risk in young women Asia. proteins contribute to genomic stability through homologous recombination (HR)-mediated double-strand DNA break repair cooperation other HR-related proteins. In this study, we analyzed the targeted sequencing data Korean patients gBRCA1/2 investigate alterations genes their clinical implications.
This study aimed at developing a novel multiplex polymerase chain reaction (PCR) primer set for identification of the potentially probiotic Bifidobacterium species B. adolescentis, animalis subsp. (B. animalis), bifidum, breve, longum biovar infantis infantis), lactis lactis, longum) and pseudolongum. The comprised specific conserved primers was derived from integrated sequences 16S 23S rRNA genes intergenic spacer region (ISR) each species. It could detect identify type strains isolates...
Purpose KX-01 is a novel dual inhibitor of Src and tubulin. Unlike previous inhibitors that failed to show clinical benefit during treatment breast cancer, can potentially overcome the therapeutic limitations current through inhibition both The present study further evaluates activity mechanism in vitro vivo. Materials Methods antitumor effect triple negative cancer (TNBC) cell lines was determined by MTT assay. Wound healing immunofluorescence assays were performed evaluate action...
Abstract Although the efficacy of trastuzumab deruxtecan (T-DXd) against HER2-positive gastric cancers (GCs) has driven its clinical application, precise mechanisms governing immunomodulatory role remain unclear. In this study, we examined immune-related action T-DXd in GC cells. exhibited potent antitumor effects cells across diverse HER2 expression levels by inducing DNA damage and apoptosis. Activation response led to increased PD-L1 expression. RNA-Seq analysis revealed that modulated...
This study identified microRNAs involved in myocardial infarction (MI) through a novel system-level approach using RNA sequencing data an MI mouse model. the extraction of DEGs and DEmiRs from RNA-seq sham samples subsequent selection two miRNAs: miR-30-5p (family) miR-142a-5p, which were downregulated upregulated MI, respectively. Gene Set Enrichment Analysis (GSEA) predicted targets miRNAs suggested that apoptosis is essential gene ontology (GO)-associated term. In vitro functional assays...
Abstract Cardiomyocyte ploidy has been described but remains obscure in cardiac interstitial cells. Ploidy of c-kit+ cells was assessed using confocal, karyotypic, and flow cytometric technique. Notable differences were found between rodent (rat, mouse) possessing mononuclear tetraploid (4n) content, compared to large mammals (human, swine) with diploid (2n) content. In-situ analysis, confirmed fresh isolates, revealed content human a mixture mouse. Downregulation the p53 signaling pathway...