A5083477042- Pancreatic function and diabetes
- Diabetes and associated disorders
- Metabolism, Diabetes, and Cancer
- Hippo pathway signaling and YAP/TAZ
- Wnt/β-catenin signaling in development and cancer
- Neuroscience and Neuropharmacology Research
- Epigenetics and DNA Methylation
- Anesthesia and Pain Management
- Genetics and Neurodevelopmental Disorders
- Cellular transport and secretion
- Diabetes Management and Research
- Cancer-related gene regulation
- Pluripotent Stem Cells Research
- Airway Management and Intubation Techniques
- Adipose Tissue and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Pain Mechanisms and Treatments
- Sinusitis and nasal conditions
- Cardiac Arrest and Resuscitation
- RNA Research and Splicing
- Asthma and respiratory diseases
- Barrier Structure and Function Studies
- Congenital heart defects research
- Retinal Development and Disorders
- Pancreatitis Pathology and Treatment
Jichi Medical University
2015-2025
International University of Health and Welfare
2018-2025
National Center for Global Health and Medicine
2013-2024
Center for Global Health
2024
Kagoshima University
2021
Osaka Medical and Pharmaceutical University
2004-2017
Harvard University
2005-2015
Joslin Diabetes Center
2005-2015
University of Tsukuba
2015
Tokyo Medical and Dental University
2000-2004
MAGI-1 is a membrane-associated guanylate kinase protein at tight junctions in epithelial cells. It interacts with various molecules and functions as scaffold cell junctions. We report here novel MAGI-1-binding that we named junctional adhesion molecule 4 (JAM4). JAM4 belongs to an immunoglobulin family. was colocalized ZO-1 kidney glomeruli intestinal Biochemical vitro studies revealed bound but not ZO-1, whereas JAM1 did bind MAGI-1. interacted each other formed clusters COS-7 cells when...
The adult pancreas has considerable capacity to regenerate in response injury. We hypothesized that after partial pancreatectomy (Px) rats, pancreatic-duct cells serve as a source of regeneration by undergoing reproducible dedifferentiation and redifferentiation. support this hypothesis the detection an early loss ductal differentiation marker Hnf6 mature ducts, followed transient appearance areas composed proliferating ductules, called foci regeneration, which subsequently form new...
Dedifferentiation has been implicated in β cell dysfunction and loss rodent diabetes. However, the pathophysiological significance humans remains unclear. To elucidate this, we analyzed surgically resected pancreatic tissues of 26 Japanese subjects with diabetes 11 nondiabetic subjects, who had overweight during adulthood but no family history The diabetic were subclassified into 3 disease stage categories, early, advanced, intermediate. Despite numerical changes endocrine cells...
Significance Recently, the potassium voltage-gated channel, KQT-like subfamily Q, member1 ( KCNQ1 ) gene has received much attention as a candidate susceptibility for type 2 diabetes in Asian, European, and other populations. The molecular mechanism underlying association of with onset remained unclear; however, we have now found that paternal allelic mutation Kcnq1 results up-regulation neighboring imprinted cyclin-dependent kinase inhibitor 1C Cdkn1c ), cell cycle inhibitor, pancreatic...
Insulin-dependent diabetes in patients with Wolfram syndrome (WS; OMIM 222300) has been linked to endoplasmic reticulum (ER) stress caused by WFS1 gene mutations. However, the pathological process of ER stress–associated β cell failure remains be fully elucidated. Our results indicate loss lineage and subsequent dedifferentiation as mechanisms underlying functional mass deficits WS. An immunohistochemical analysis human pancreatic sections from deceased individuals WS revealed a...
Synaptic scaffolding molecule (S-SCAM) is a synaptic membrane-associated guanylate kinase with inverted domain organization (MAGI) that interacts NMDA receptor subunits and neuroligin. In epithelial cells, the non-neuronal isoform of S-SCAM (MAGI-1) localized at tight or adherens junctions. Recent studies have revealed polarized targeting MAGI-1 to lateral membrane mediated by its C-terminal region beta-catenin in cells. this article, we report neurons. beta-Catenin coimmunoprecipitated from...
Mammalian MafA/RIPE3b1 is an important glucose-responsive transcription factor that regulates function, maturation, and survival of beta-cells. Increased expression MafA results in improved glucose-stimulated insulin secretion beta-cell function. Because a highly phosphorylated protein, we examined whether regulating activity protein kinases can increase by enhancing its stability. We demonstrate stability MIN6 cells isolated mouse islets regulated both p38 MAPK glycogen synthase kinase 3....
A neural plakophilin-relatedarmadillo repeat protein (NPRAP)/δ-catenin interacts with one of Alzheimer disease-related gene products, presenilin 1. We have previously reported the interaction NPRAP/δ-catenin synaptic scaffolding molecule, which is involved in assembly components. also E-cadherin and β-catenin implicated organization cell-cell junctions. p0071, a ubiquitous isoform NPRAP/δ-catenin, localized at desmosomes HeLa A431 cells adherens junctions Madin-Darby bovine kidney cells....
OBJECTIVE—Diabetes results from a deficiency of functional β-cells due to both an increase in β-cell death and inhibition replication. The molecular mechanisms responsible for these effects susceptible individuals are mostly unknown. objective this study was determine whether gene critical cell division survival cancer cells, survivin, might also be important β-cells. RESEARCH DESIGN AND METHODS—We generated mice harboring conditional deletion survivin pancreatic endocrine cells using with...
Abstract. Purpose: Hyperglycaemia has been identified as major risk factor for diabetic retinopathy (DR). It is widely accepted that the progression of DR mainly due to a local imbalance pro‐ versus anti‐angiogenic factors in retina. In this study, we investigated whether retinal pigment epithelial (RPE) cells produced pro‐angiogenic under high glucose (HG) conditions vitro . Methods: Cultured human endothelial (RE) were exposed conditioned medium from epithelium (ARPE‐19) grown HG and...
Pathological complete response (pCR) is considered to be a useful prognostic marker for neoadjuvant chemotherapy improve the survival rate of patients with operable breast cancer. In present study, we identified differentially expressed microRNAs (miRNAs) between pCR and non-pCR groups human epidermal growth factor receptor 2 (HER2)-positive cancer who received trastuzumab. Expression profiles were examined by miRNA microarrays using total RNA extracted from formalin-fixed, paraffin-embedded...
The postnatal proliferation and maturation of insulin-secreting pancreatic β-cells are critical for glucose metabolism disease development in adults. Elucidation the molecular mechanisms underlying these events will be beneficial to direct differentiation stem cells into functional β-cells. Maturation is accompanied by increased expression MafA, an insulin gene transcription factor. Transcriptome analysis MafA knockout islets revealed required several molecules β-cell function, including...
Chemotherapy‑induced nausea and vomiting is a serious adverse side‑effect of anthracycline‑based chemotherapy regimens, in patients with breast cancer. A combination three drugs, 5‑hydroxytryptamine (5‑HT3) receptor antagonist, aprepitant dexamethasone, recommended for antiemetic therapy. Palonosetron (PALO), novel 5‑HT3 antagonist has been identified to be effective against delayed vomiting. In this study, the results PALO who received were compared that granisetron (GRA) using crossover...
Brain-enriched guanylate kinase-associated protein (BEGAIN) interacts with postsynaptic density (PSD)-95/synapse-associated (SAP) 90. In immunohistochemistry and immunocytochemistry, BEGAIN was detected in nuclei at synapses neurons. Nuclear localization also confirmed through subcellular fractionation. localized exclusively when expressed epithelial cells. These findings led us to analyze the mechanism determine of Green fluorescent (GFP)-tagged appeared first subsequently accumulated...
Brain-specific angiogenesis inhibitor (BAI)-associated protein (BAP)1 (also called membrane-associated guanylate kinase [MAGI]-1) is composed of six PSD-95/ Dlg-A/ZO-1 (PDZ) domains, two WW and one (GK) domain. We previously reported that BAP1 localized at tight junctions in Madine Darby canine kidney (MDCK) cells intestinal epithelial cells. Here, we have determined the localization normal rat (NRK) do not form junctions. was colocalized with E-cadherin along lateral membrane, suggesting...
Brain-specific angiogenesis inhibitor (BAI)-associated protein (BAP)1 (also called membrane-associated guanylate kinase [MAGI]-1) is composed of six PSD-95/Dlg-A/ZO-1 (PDZ) domains, two WW and one (GK) domain. We previously reported that BAP1 localized at tight junctions in Madine Darby canine kidney (MDCK) cells intestinal epithelial cells. Here, we have determined the localization normal rat (NRK) do not form junctions. was colocalized with E-cadherin along lateral membrane, suggesting its...