A5051229881- Advanced Sensor and Energy Harvesting Materials
- Conducting polymers and applications
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Spaceflight effects on biology
- Cellular Mechanics and Interactions
- Supramolecular Self-Assembly in Materials
- Mesenchymal stem cell research
- Enzyme-mediated dye degradation
- Fungal Biology and Applications
- Analytical Chemistry and Sensors
- Biofuel production and bioconversion
- Photochromic and Fluorescence Chemistry
- Microbial Metabolic Engineering and Bioproduction
- Microbial bioremediation and biosurfactants
- Leech Biology and Applications
- Polymer Surface Interaction Studies
- Biomedical and Engineering Education
- Electron and X-Ray Spectroscopy Techniques
- Ga2O3 and related materials
- Planarian Biology and Electrostimulation
- Microbial Metabolism and Applications
Université Claude Bernard Lyon 1
2019-2022
Biologie, ingénierie et imagerie pour l'Ophtalmologie
2021-2022
Inserm
2019-2022
Université Jean Monnet
2019-2022
Centre National de la Recherche Scientifique
2021-2022
Matériaux Ingénierie et Science
2021-2022
Linköping University
2016-2021
Mines Saint-Étienne
2017-2021
University of Cambridge
2018-2020
Institute Cancer De La Loire Lucien Neuwirth
2020
This work reports the design of a live‐cell monitoring platform based on macroporous scaffold conducting polymer, poly(3,4‐ethylene dioxythiophene):poly(styrenesulfonate). The polymer scaffolds support 3D cell cultures due to their biocompatibility and tissue‐like elasticity, which can be manipulated by inclusion biopolymers such as collagen. Integration media perfusion tube inside enables homogenous spreading fluid transport throughout scaffold, ensuring long term viability. also allows for...
We report development of the first biomimetic transistor in a tube for continuous monitoring 3D cell cultures.
This study aims at investigating the behavior in terms of proliferation and skeletal muscle differentiation capability two myoblastic cell lines, C2C12 H9c2, on both isotropic anisotropic electrospun nanofibrous poly(hydroxybutyrate) (PHB) scaffolds, as well PHB films polystyrene controls. After a careful characterization matrices surface morphology, roughness mechanical properties, rate lines to form myotubes were evaluated. Genetic analyses also performed order assess level cells different...
Conducting polymer scaffolds can promote cell growth by electrical stimulation, which is advantageous for some specific type of cells such as neurons, muscle, or cardiac cells. As an additional feature, the measure their impedance has been demonstrated a tool to monitor within scaffold. In this work, we present innovative conducting porous based on poly(3,4-ethylenedioxythiophene) (PEDOT):xanthan gum instead well-known PEDOT:polystyrene sulfonate scaffolds. These novel combine conductivity...
Bones have been shown to exhibit piezoelectric properties, generating electrical potential upon mechanical deformation and responding stimulation with the generation of stress. Thus, effects on bone tissue engineering extensively studied. However, in regeneration applications, only few studies focused use electroactive 3D biodegradable scaffolds at interphase stem cells. Here a method is described combine capabilities 3D‐printed macroporous medical grade polycaprolactone (PCL)...
A new technique for patterning and tuning of electrochromic properties conductive polymers was used to make dual image displays.
A novel patterning technique of conductive polymers produced by vapor phase polymerization is demonstrated. The method involves exposing an oxidant film to UV light which changes the local chemical environment and subsequently kinetics. This procedure used control conductivity in conjugated polymer poly(3,4‐ethylenedioxythiophene):tosylate more than six orders magnitude addition producing high‐resolution patterns optical gradients. mechanism behind modulation kinetics irradiation as well...
While most of the studies on molecular machines have been performed in solution, interfacing these supramolecular systems with solid-state nanostructures and materials is very important view their utilization sensing components working by chemical photonic actuation. Host polymeric materials, particularly polymer nanofibers, enable manipulation functional molecules constituting machines, provide a way to induce control organization. Here, we present electrospun nanocomposites embedding...
In the last decade, importance of topographic properties extracellular environments has been shown to be essential addressing cell response, especially when replacing damaged tissues with functional constructs obtained in vitro. current study, densely packed sub-micron poly(3-hydroxybutyrate) (PHB) fibres were electrospun random and parallel orientations. PC12 pheochromocytoma cells that mimic central dopaminergic neurons represent a model for neuronal differentiation cultured on...
Low temperature aqueous chemical synthesis (LT-ACS) of zinc oxide (ZnO) nanorods (NRs) has been attracting considerable research interest due to its great potential in the development light-emitting diodes (LEDs). The influence molar ratio acetate (ZnAc): KOH as a ZnO seed layer precursor on density interface defects and hence presence non-radiative recombination centers LT-ACS NRs/GaN LEDs systematically investigated. material quality as-prepared quantitatively deduced by X-ray...
Bone exhibits piezoelectric properties. Thus, electrical stimulations such as pulsed electromagnetic fields (PEMFs) and stimuli-responsive properties of scaffolds have been investigated separately to evaluate their efficacy in supporting osteogenesis. However, current understanding cells responding under the combined influence PEMF is still lacking. Therefore, this study, we fabricated by functionalization polycaprolactone-tricalcium phosphate (PCL-TCP) films with a polyvinylidene fluoride...
Abstract Cell fate is largely determined by interactions that occur at the interface between cells and their surrounding microenvironment. For this reason, especially in field of tissue‐engineering, there a growing interest developing techniques allow evaluating cell–material interaction nanoscale, particularly focusing on cell adhesion processes. While for 2D culturing systems consolidated series tools already satisfy need, 3D environments, more closely recapitulating complex vivo...
Abstract Osteoporosis is a skeletal disease characterized by bone loss and microarchitectural deterioration. The combination of smart materials stem cells represents new therapeutic approach. In the present study, highly porous scaffolds are prepared combining conducting polymer PEDOT:PSS with collagen type I, most abundant protein in bone. inclusion proves to be an effective way modulate their mechanical properties it induces increase scaffolds’ electrochemical impedance. biomimetic support...
Due to the ageing population, there is a steadily increasing incidence of osteoporosis and osteoporotic fractures. As conventional pharmacological therapy options for are often associated with severe side effects, bone grafts still considered clinical gold standard. However, availability viable, autologous limited making alternative cell-based strategies promising therapeutic alternative. Adipose-derived stem cells (ASCs) readily available population mesenchymal stem/stromal (MSCs) that can...
The anti-inflammatory and immunomodulatory properties of human mesenchymal stromal cells (MSCs) are a focus within regenerative medicine. However, 2D cultivation MSCs for extended periods results in abnormal cell polarity, chromosomal changes, reduction viability, altered differentiation potential. As an alternative, various 3D hydrogels have been developed which mimic the endogenous niche MSCs. Nevertheless, imaging embedded often suffers from low signal-to-noise ratios can be at least...
Stem cell osteogenic differentiation is a complex process, associated with number of events such as the secretion collagen type I, osteopontin, osteonectin, osteocalcin and Bone Morphogenic Protein 2 (BMP-2). These molecules can be used markers to monitor stem fate while studying effects specific treatment (e.g. electrical stimulation). Currently available techniques, evaluation expression levels genes end-point biochemical assays, do not allow real-time monitoring cellular processes,...
scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et recherche français étrangers, laboratoires publics privés.
The use of adult stem/progenitor cells is a challenge in current research about kidney functional regeneration. In this framework, material‐induced stem cell differentiation can become new paradigm to promote advances therapies. Here, the effects both isotropic and anisotropic fibrous topographies on podocyte renal (RSCs) from human donors are investigated. proliferation rate RSCs analyzed, immunofluorescence genetic analyses specific markers (Wilms’ tumor 1 gene, nephrin, podocin) performed...
The influence of novel nanostructured anisotropically electrospun poly(hydroxy butyrate) matrices on skeletal and cardiac muscle-like cell proliferation differentiation was investigated, in comparison with isotropic no-topographically cues-provided substrates. After the matrix characterization, terms surface SEM imaging mechanical properties, different substrates evaluated. Myogenin F-actin staining at several time-points suggested that aligned nanofibers promote both types. Moreover,...