A5090820174- Research in Cotton Cultivation
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Ubiquitin and proteasome pathways
- Molecular Biology Techniques and Applications
- Plant Reproductive Biology
- Plant biochemistry and biosynthesis
- Plant tissue culture and regeneration
- Insect Resistance and Genetics
- Plant responses to water stress
- Lipid metabolism and biosynthesis
- Nanoparticles: synthesis and applications
- Silk-based biomaterials and applications
- Insect-Plant Interactions and Control
- Plant and Fungal Interactions Research
- Endoplasmic Reticulum Stress and Disease
- Cocoa and Sweet Potato Agronomy
- Antioxidant Activity and Oxidative Stress
- Studies on Chitinases and Chitosanases
- Rice Cultivation and Yield Improvement
- Plant responses to elevated CO2
Zhengzhou University
2021-2025
Chinese Academy of Agricultural Sciences
2016-2025
Cotton Research Institute
2015-2024
Nanjing Agricultural University
2023-2024
Zhengzhou Fruit Research Institute
2024
Anyang Institute of Technology
2023
State Key Laboratory of Cotton Biology
2017-2021
Shihezi University
2015
Jiangsu Academy of Agricultural Sciences
2012
Abstract The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 system has been widely used for genome editing in various plants because of its simplicity, high efficiency and design flexibility. However, to our knowledge, there is no report on the application CRISPR/Cas9-mediated targeted mutagenesis cotton. Here, we upland cotton ( Gossypium hirsutum L., hereafter cotton) using CRISPR/Cas9 system. We designed two guide RNAs target distinct sites Cloroplastos alterados...
Recent researches on long noncoding RNAs (lncRNAs) have expanded our horizon of gene regulation and the cellular complexity. However, number, characteristics expression patterns lncRNAs remain poorly characterized how these biogenesis are regulated in response to drought stress cotton still largely unclear. In study, using a reproducibility-based RNA-sequencing bioinformatics strategy analyze 9 samples under three different environment stresses (control, re-watering, replications), we...
As one of the cash crops, cotton is facing threat abiotic stress during its growth and development. It has been reported that melatonin involved in plant defense against salt stress, but whether can improve tolerance molecular mechanism remain unclear. We investigated role by silencing synthesis gene exogenous application upland cotton. In this study, applicating seedlings. The content endogenous was different varieties with tolerance. inhibition biosynthesis related genes resulted decrease...
Abstract Background Cotton is an important industrial crop and a pioneer for saline-alkali land restoration. However, the molecular mechanism underlying cotton response to salt not completely understood. Methods Here, we used metabolome data transcriptome analyze tolerance regulatory network of metabolic biomarkers. Results In this study, was stressed at 400 m M NaCl 0 h, 3 24 h 48 h. interfered with gene expression, altered metabolite contents affected plant growth. Metabolome analysis...
We identified 131 AP2/ERF (APETALA2/ethylene-responsive factor) genes in material from peach using the gene sequences of amino acids Arabidopsis thaliana (Brassicaceae) as probes. Based on number domains and individual characteristics, superfamily can be classified broadly into three families, ERF (ethylene-responsive factor), RAV (related to ABI3/VP1), AP2 (APETALA2), containing 104, 5, 21 members, respectively, along with a solo (ppa005376m). The 104 family were further divided 11 groups...
DNA methylation, with a cryptic role in genome stability, gene transcription and expression, is involved the drought response process plants, but complex regulatory mechanism still largely unknown. Here, we performed whole-genome bisulfite sequencing (WGBS) identified long non-coding RNAs on cotton leaves under stress re-watering treatments. We obtained 31,223 30,997 differentially methylated regions (representing 2.48% of genome) after treatments, respectively. Our data also showed that...
Upland cotton (Gossypium hirsutum L.), an important source of natural fiber, can tolerate relatively high salinity and drought stresses. In the present study, a plasma membrane Na+/H+ antiporter gene, GhSOS1, was cloned from salt-tolerant genotype G. hirsutum, Zhong 9807. The expression level GhSOS1 in roots significantly upregulated presence concentrations NaCl (200 mM), while its transcript abundance increased when exposed to low temperature Localization analysis using onion epidermal...
Basic leucine zipper (bZIP) transcription factor plays an important role in various biological processes, such as response to biotic and abiotic stresses. In this study we performed a systematic investigation analysis of bZIP gene family Gossypium hirsutum predict their functions different A total 207 genes were identified from genome classified into 13 subfamilies through phylogenetic analysis, which was testified by the conserved motifs exon-intron structures. Annotation GHbZIPs based on...
How to develop new cotton varieties possessing high yield traits of Upland and superior fiber quality Sea Island remains a key task for breeders researchers. While multiple attempts bring in little significant progresses, the development Chromosome Segment Substitution Lines (CSSLs) from Gossypium barbadense G. hirsutum background provided ideal materials aforementioned breeding purposes upland improvement. Based on excellent performance relatively clear chromosome substitution segments...
High concentrations of heavy metals in the soil should be removed for environmental safety. Cadmium (Cd) is a metal that pollutes when its concentration exceeds 3.4 mg/kg. Although potential use cotton to remediate Cd-polluted soils known, little understood about molecular mechanisms Cd tolerance. In this study, transcriptome analysis was used identify tolerance genes and their cotton. We exposed plants excess identified 4627 differentially expressed (DEGs) root, 3022 DEGs stem 3854 leaves...
Alkaline stress is one of the abiotic stresses limiting cotton production. Though RNA-Seq analyses, have been conducted to investigate genome-wide gene expression in response alkaline plants, sodium bicarbonate (NaHCO3) stress-related genes has not reported. To explore mechanisms this stress, we used next-generation sequencing (NGS) technology study transcriptional changes under NaHCO3 stress. A total 18,230 and 11,177 differentially expressed (DEGs) were identified roots leaves,...
Abstract Verticillium dahliae is a soil‐borne, phytopathogenic fungus that causes wilt in cotton. Salt tolerance of cotton plants suffering from was significantly improved, but the mechanism unknown. In this study, we built symbiont named Cotton≡VD by immersing plant roots into liquid for four days. This resulted strong interaction between hyphae and roots. Under NaCl treatment 24 h, displayed less leaf wilting improved root growth compared with control. Biochemical tests SOD, POD CAT showed...
Soil salinization is a global constraint that significantly hampers agricultural production, with cotton being an important cash crop not immune to its detrimental effects. The rhizosphere microbiome plays critical role in plant health and growth, which assists plants resisting adverse abiotic stresses including soil salinization. This study explores the impact of on cotton, effects yield, physical chemical properties, as well bacterial community structures. results β-diversity analysis...
The U-box gene encodes a ubiquitin ligase that contain domain. plant (PUB) plays an important role in the response to stresses, but few reports about PUBs cotton were available. Therefore research on is of great importance and necessity when studying mechanisms stress- tolerance cotton.In this study, we identified 93, 96, 185 208 from four sequenced species G. raimondii (D5), arboreum (A2), hirsutum (AD1) barbadense (AD2), respectively. Prediction analysis subcellular localization showed...
Serotonin N-acetyltransferase (SNAT) is a key enzyme in the biosynthesis of melatonin, and plays an important role regulation melatonin synthesis. The study SNAT great significance to understand function melatonin. In this study, we analyzed structural characteristics, phylogenetic relationship, gene structure, expression pattern, evolutionary relationship stress response members family upland cotton through bioinformatics. A putative n-acetyltransferase GhSNAT3D was identified,...
As an important source of fiber and edible oil, cotton has great economic value. In comparison to their individual studies, association differentiation between salt alkaline tolerance not been focused yet by scientists. We have used next-generation RNA-Seq technique analyze transcriptional changes under stresses in cotton. Overall, 25,929 6,564 differentially expressed genes (DEGs) were identified roots leaves, respectively. Gene functional annotation showed that involving ionic homeostasis...
Background-independent (BI) and stably expressed (SE) quantitative trait loci (QTLs) were identified using two sets of introgression lines across multiple environments. Genetic background more greatly affected fiber quality traits than environmental factors. Sixty-one SE-QTLs, including BI-QTLs, novel 48 seven previously reported. Cotton are controlled by QTLs susceptible to influence. Fiber improvement is an essential goal in cotton breeding but hindered limited knowledge the genetic basis...
The phytophagous mite Tetranychus truncatus is a serious pest in East Asia but has relatively narrower host range than the urticae, which can feed on over 1200 plant species. Here, we generated high-quality chromosomal level genome of T. and compared it with that an emphasis genes related to detoxification chemoreception, explore genomic basis underlying evolution range. We also conducted population genetics analyses (in 86 females from 10 populations) transfer experiments 4 investigate...
Abstract The VIM (belonged to E3 ubiquitin ligase) gene family is crucial for plant growth, development, and stress responses, yet their role in salt remains unclear. We analyzed phylogenetic relationships, chromosomal localization, conserved motifs, structure, cis -acting elements, expression patterns of the four cotton varieties. Our findings reveal 29, 17, 14 members Gossypium hirsutum (G.hirsutum), barbadense (G.barbadense), arboreum (G.arboreum), raimondii (G. raimondii) , respectively,...
Naturally existing colored cotton was far from perfection due to having genetic factors for lower yield, poor fiber quality and monotonous color. These posed a challenge breeding innovation. To identify novel quantitative trait loci (QTL) color along with understanding of correlation between in cotton, RIL two F2 populations were generated crosses among Zong128 (Brown cotton) white lines which then analyzed four environments. Two stable major QTLs (qLC-7-1, qFC-7-1) lint fuzz detected...
Abstract Background Cotton ( Gossypium hirsutum ) is considered a fairly salt tolerant crop however, salinity can still cause significant economic losses by affecting the yield and deteriorating fiber quality. We studied salt-tolerant upland cotton cultivar under temporal stress to unfold tolerance molecular mechanisms. Biochemical response (400 mM) was measured at 0 h, 3 12 24 h 48 post intervals single-molecule long-read sequencing technology from Pacific Biosciences (PacBio) combined with...
Choline kinase (CK) gene plays an important role in plants growth, development and resistance to stress. It mainly regulates the biosynthesis of phosphatidylcholine. This study aims explore structure-function relationship, provide a framework for functional validation biochemical characterization various CK genes. Our analysis showed that 87 genes were identified cotton 7 diploid plants, which 43 encode proteins 4 species, 13 Gossypium hirsutum. Most GhCK are affected by abiotic stress...