A5103260889- Research in Cotton Cultivation
- Plant Virus Research Studies
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant and Fungal Interactions Research
- Plant Molecular Biology Research
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Genetic Mapping and Diversity in Plants and Animals
- Neurobiology and Insect Physiology Research
- Retinal Development and Disorders
- Virus-based gene therapy research
- vaccines and immunoinformatics approaches
- Geothermal Energy Systems and Applications
- Insect Resistance and Genetics
- Hydrocarbon exploration and reservoir analysis
- Hearing, Cochlea, Tinnitus, Genetics
- Pharmacogenetics and Drug Metabolism
- Hippo pathway signaling and YAP/TAZ
- Cocoa and Sweet Potato Agronomy
- Reservoir Engineering and Simulation Methods
- Plant Gene Expression Analysis
- Synthetic Aperture Radar (SAR) Applications and Techniques
- Muscle Physiology and Disorders
- Genetics and Plant Breeding
Chinese Academy of Agricultural Sciences
2015-2024
Shanghai Veterinary Research Institute
2023-2024
Henan Institute of Geological Survey
2024
Beijing Institute of Technology
2024
Cotton Research Institute
2015-2023
Shanghai Academy of Agricultural Sciences
2023
Animal Science Research Institute
2023
Yangzhou University
2023
Peking University Shenzhen Hospital
2016-2022
Hong Kong University of Science and Technology
2016-2022
Upland Cotton (Gossypium hirsutum) is one of the most important worldwide crops it provides natural high-quality fiber for industrial production and everyday use. Next-generation sequencing a powerful method to identify single nucleotide polymorphism markers on large scale construction high-density genetic map quantitative trait loci mapping. In this research, recombinant inbred lines population developed from two upland cotton cultivars 0–153 sGK9708 was used construct through specific...
It is of great importance to identify quantitative trait loci (QTLs) controlling fiber quality traits and yield components for future marker-assisted selection candidate gene function identifications. In this study, two kinds in 231 F6:8 recombinant inbred lines (RILs), derived from an intraspecific cross between Xinluzao24, a cultivar with elite quality, Lumianyan28, wide adaptability high potential, were measured nine environments. This RIL population was genotyped by 122 SSR 4729 SNP...
The identification of quantitative trait loci (QTLs) that are stable and consistent across multiple environments populations plays an essential role in marker-assisted selection (MAS). In the present study, we used 28,861 simple sequence repeat (SSR) markers, which included 12,560 Gossypium raimondii (D genome) sequence-based SSR markers to identify polymorphism between two upland cotton strains 0–153 sGK9708. A total 851 polymorphic primers were finally selected genotype 196 recombinant...
Cotton is widely cultivated globally because it provides natural fibre for the textile industry and human use. To identify quantitative trait loci (QTLs)/genes associated with quality yield, a recombinant inbred line (RIL) population was developed in upland cotton. A consensus map covering whole genome constructed three types of markers (8295 markers, 5197.17 centimorgans (cM)). Six yield traits were evaluated 17 environments, 983 QTLs identified, 198 which stable mainly distributed on...
Verticillium wilt (VW), also known as "cotton cancer," is one of the most destructive diseases in global cotton production that seriously impacts fiber yield and quality. Despite numerous attempts, little significant progress has been made improving VW resistance upland cotton. The development chromosome segment substitution lines (CSSLs) from Gossypium hirsutum × G. barbadense emerged a means simultaneously developing new varieties with high-yield, superior fiber, to VW. In this study,...
Cotton (Gossypium hirsutum L.) is an important agricultural crop that provides renewable natural fiber resources for the global textile industry. Technological developments in industry and improvements human living standards have increased requirement supplies better quality cotton. Upland cotton 0–153 elite cultivar harboring strong strength genes. To conduct quantitative trait locus (QTL) mapping 0–153, we developed a population of 196 recombinant inbred lines (RILs) from cross between...
Pectin methylesterase (PME, EC 3.1.1.11) is a hydrolytic enzyme that utilizes pectin as substrates, and plays significant role in regulating reconstruction thereby plant growth. one of the important components cell wall, which forms main structural material cotton fiber. In this research, genome information was used to identify PMEs. We identified 80 (GaPME01-GaPME80) PME genes from diploid G. arboreum (A genome), 78 (GrPME01-GrPME78) raimondii (D 135 (GhPME001-GhPME135) tetraploid hirsutum...
Feline coronavirus (FCoV) is an unsegmented, single-stranded RNA virus belonging to the Alphacoronavirus genus. It can cause fatal feline infectious peritonitis (FIP) in cats of any ages. Currently, there are no effective prevention and control measures against FCoV. In this study, we developed a recombinant adenovirus vaccine, AD5-N, based on nucleocapsid(N) protein The immunogenicity AD5-N was evaluated through intramuscular immunization 6-week-old Balb/c mice 9–12 months old cats....
Introduction Chronic kidney disease (CKD) is a globally prevalent condition characterized by high morbidity and progressive course that often culminates in end-stage renal (ESRD), necessitating dialysis or transplantation. In recent years, genetic factors have received increasing attention the pathogenesis of CKD, particularly among patients with unexplained dysfunction. Genetic screening has emerged as valuable diagnostic tool. Mutations TMEM216 gene, pathogenic variant associated...
Cotton ( Gossypium sp.) is an important worldwide cash crop that provides a competitive renewable natural fiber supply for the demands of textile industry. The development new technologies and improvement living standards increase both quantity quality. ‘0–153’ upland cotton cultivar with excellent quality derived from Asiatic sources, especially regards to strength. To identify quantitative trait loci (QTLs) strength in this line, recombinant inbred line population consisting 196 lines was...
Background-independent (BI) and stably expressed (SE) quantitative trait loci (QTLs) were identified using two sets of introgression lines across multiple environments. Genetic background more greatly affected fiber quality traits than environmental factors. Sixty-one SE-QTLs, including BI-QTLs, novel 48 seven previously reported. Cotton are controlled by QTLs susceptible to influence. Fiber improvement is an essential goal in cotton breeding but hindered limited knowledge the genetic basis...
Background Porcine deltacoronavirus (PDCoV), a novel swine enteropathogenic coronavirus, challenges the global industry. Currently, there are no approaches preventing from PDCoV infection. Methods A new strain named JS2211 was isolated. Next, dimer receptor binding domain of spike protein (RBD-dimer) expressed using prokaryotic expression system, and nanoparticle containing RBD-dimer ferritin (SC-Fe) constructed SpyTag/SpyCatcher system. Finally, immunoprotection RBD-Fe nanoparticles...
Class III myosins (Myo3) and actin-bundling protein Espin play critical roles in regulating the development maintenance of stereocilia vertebrate hair cells, their defects cause hereditary hearing impairments. Myo3 interacts with Espin1 through its tail homology I motif (THDI), however it is not clear how specifically acts to regulate actin bundle assembly stabilization. Here we discover that THDI contains a pair repeat sequences capable independently strongly binding ankyrin repeats Espin1,...
To obtain more information of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) transmission via faeces in/between farms, 360 swine faecal samples were randomly collected from different farms in China 2017 to 2019. Sixty-two ORF5 genes amplified by PCR 120 positive identified real-time RT-PCR further characterized sequencing. Phylogenetic analysis based on the gene revealed that these strains can be divided into four lineages: lineage 1 (NADC30-like), 3 (QYYZ-like), 5.1...
Abstract Background O-methyltransferases (OMTs) are an important group of enzymes that catalyze the transfer a methyl from S-adenosyl-L-methionine to their acceptor substrates. OMTs divided into several groups according structural features. In Gossypium species, they involved in phenolics and flavonoid pathways. Phenolics defend cellulose fiber dreadful external conditions biotic abiotic stresses, promoting strength growth plant cell wall. Results An OMT gene family, containing total 192...
The idea of enhanced methanol production from cell wall by pectin methyl esterase enzymes (PME) combined with expression cry genes Bacillus thuringiensis as a strategy to improve insect pest control in cotton is presented. We constructed cassette containing two (cry1Fa and Cry32Aa) pme genes, one Arabidopsis thaliana (AtPME), other Aspergillus. niger (AnPME) pCAMBIA1301 plant vector using CAMV-35S promoter. This construction was transformed Eagle-2 variety shoot apex-cut...
Fiber quality and yield are important traits of cotton. Quantitative trait locus (QTL) mapping is a prerequisite for marker-assisted selection (MAS) in cotton breeding. To identify QTLs fiber traits, 4 backcross-generation populations (BC1F1, BC1S1, BC2F1, BC3F0) were developed from an interspecific cross between CCRI36 (Gossypium hirsutum L.) Hai1 (G. barbadense L.). A total 153 identified based on data the BC1F1, BC2F1 BC3F0 field population artificial disease nursery using high-density...
Abstract Background Fructose-1,6-bisphosphatase (FBP) is a key enzyme in the plant sucrose synthesis pathway, Calvin cycle, and plays an important role photosynthesis regulation green plants. However, no systemic analysis of FBPs has been reported Gossypium species. Results A total 41 FBP genes from four species were identified analyzed. These sorted into two groups seven subgroups. revealed that family under purifying selection pressure rendered members as being conserved evolutionarily,...
Abstract The idea of enhanced methanol production from cell wall by pectin methyl esterase enzymes (PME) combined with expression cry genes Bacillus thuringiensis as a strategy to improve pests control in cotton is presented. We constructed cassette containing two (cry1Fa and Cry32Aa) pme genes, one Arabidopsis thaliana (AtPME), other Aspergillus niger (AnPME) pCAMBIA1301 plant vector using CAMV-35S promoter. This construction was transformed Eagle-2 variety shoot apex-cut...
Cotton is a significant economic crop that plays an indispensable role in many domains. Gossypium hirsutum L. the most important fiber worldwide and contributes to more than 95% of global cotton production. Identifying stable quantitative trait locus (QTLs) controlling quality yield related traits are necessary prerequisites for marker-assisted selection (MAS). A genetic linkage map was constructed with 312 simple sequence repeat (SSR) loci 35 groups using JoinMap 4.0; spanned 1 929.9 cM,...
Upland cotton is an important allotetraploid crop that provides both natural fiber for the textile industry and edible vegetable oil food or feed industry. To better understand genetic mechanism regulates biosynthesis of storage in cottonseed, we identified genes harbored major quantitative trait loci/nucleotides (QTLs/QTNs) kernel content (KOC) cottonseed via multiple linkage analyses genome-wide association studies (GWAS). In 'CCRI70' RILs, six stable QTLs were simultaneously by analysis...
The generation and differentiation of B lymphocytes (B cells) is a flexible process with many critical regulatory factors. Previous studies indicated that non-coding RNAs play multiple roles in the development lymphocytes. However, little has been known about circular RNA (circRNA) profiles their competing endogenous (ceRNA) networks B-cell differentiation. Here, four subsets were purified from single-cell suspensions mouse bone marrow. Then sequencing (RNA-Seq) was used to display...