A5086814819- Pharmacogenetics and Drug Metabolism
- Polyamine Metabolism and Applications
- Bacteriophages and microbial interactions
- Dermatology and Skin Diseases
- Peroxisome Proliferator-Activated Receptors
- Contact Dermatitis and Allergies
- Viral Infectious Diseases and Gene Expression in Insects
- Escherichia coli research studies
- Pharmaceutical studies and practices
- Amino Acid Enzymes and Metabolism
- Lipoproteins and Cardiovascular Health
- T-cell and Retrovirus Studies
- CRISPR and Genetic Engineering
- Computational Drug Discovery Methods
- Cholesterol and Lipid Metabolism
- Lipid metabolism and disorders
- Fatty Acid Research and Health
- Monoclonal and Polyclonal Antibodies Research
- HIV/AIDS drug development and treatment
- Viral Infections and Immunology Research
- Protein purification and stability
- Endoplasmic Reticulum Stress and Disease
- Food Allergy and Anaphylaxis Research
- Lipid metabolism and biosynthesis
- Enterobacteriaceae and Cronobacter Research
National University Hospital
2024
Nanyang Technological University
2022
University of British Columbia
2019
Agency for Science, Technology and Research
2016-2019
Genome Institute of Singapore
2016-2019
National University of Singapore
2019
Xenon Pharmaceuticals (Canada)
2012-2014
Amsterdam UMC Location University of Amsterdam
2012
Montreal Heart Institute
2012
Université de Montréal
2012
Aims Adverse drug reactions (ADRs) contribute to poorer patient outcomes and additional burden the healthcare system. However, data on true burden, relevant types drugs causing ADRs are lacking. The aim of this study was determine prevalence ADR‐related hospitalization in general adult population Singapore investigate their characteristics. Methods We prospectively recruited 1000 patients with unplanned admission a large tertiary‐care hospital. Two independent reviewers evaluated all...
beta-Galactosidase (GZ) is an intracellular protein that frequently used to express cloned antigens as fusion proteins in Escherichia coli. Salmonella typhimurium strain SL3261, attenuated aroA vaccine strain, was a carrier for the plasmid pXY411, which directs expression of GZ salmonellae (which do not normally produce this protein). The resulting strain--SL3261(pXY411)--expressed antigen. stable vitro and vivo did significantly alter behavior SL3261 mice. Animals intravenously vaccinated...
Genotype-guided warfarin dosing has been shown in some randomized trials to improve anticoagulation outcomes individuals of European ancestry, yet its utility Asian patients remains unresolved.An open-label, non-inferiority, 1:1 trial was conducted at three academic hospitals South East Asia, involving 322 ethnically diverse newly indicated for (NCT00700895). Clinical follow-up 90 days. The primary efficacy measure the number dose titrations within first 2 weeks therapy, with a mean...
Yeast co‐expressing human elongase and desaturase genes were used to investigate whether the same gene encodes an enzyme able desaturate n‐3 n‐6 fatty acids with or different carbon chain length. The results clearly demonstrated that a single Δ5 is active on 20:3n‐6 20:4n‐3. Endogenous Δ6 substrates generated by providing yeast radiolabelled 20:4n‐6 20:5n‐3 which, through two sequential elongations, produced 24:4n‐6 24:5n‐3, respectively. Overall, our data suggest 18:2n‐6, 18:3n‐3, 24:5n‐3.
Endothelial lipase is a phospholipase with activity against high-density lipoprotein. Although small number of mutations in LIPG have been described, the role protection atherosclerosis unclear.We identified 8 loss-of-function (LOF) individuals lipoprotein cholesterol. Functional analysis confirmed that most rare abolish vitro, indicating complete LOF, whereas 2 more common N396S and R476W reduce by ≈50%, partial LOF implying ≈50% ≈75% remaining endothelial function heterozygous mutation...
Pharmacogenomics (PGx) is increasingly being recognized as a potential tool for improving the efficacy and safety of drug therapy. Therefore, several efforts have been undertaken globally to facilitate implementation process PGx into routine clinical practice. Part these include formation working groups on research, synthesis, dissemination data creation strategies. In Asia, Southeast Asian Research Network (SEAPharm) established enable strengthen research among various communities within...
A fusion protein consisting of beta-galactosidase (GZ) to which was attached at its N-terminus the amino acid sequence corresponding residues 142-160 immunogenic VP1 foot-and-mouth disease virus (FMDV) has been expressed in E. coli. chemically synthesized section DNA inserted into a vector (pXY410) designed express proteins with carboxy terminal 1015 acids GZ. The hybrid immunopurified by GZ-specific monoclonal antibody soluble, retained full GZ activity, and induced virus-neutralizing...
Stearoyl-CoA desaturase-1 (SCD1) catalyzes de novo synthesis of monounsaturated fatty acids from saturated acids. Studies have demonstrated that rodents lacking a functional SCD1 gene an improved metabolic profile, including reduced weight gain, lower triglycerides, and insulin response. In this study, we discovered series piperazinylpyridazine-based highly potent, selective, orally bioavailable compounds. Particularly, compound 49 (XEN103) was active in vitro (mSCD1 IC(50) = 14 nM HepG2 12...
While genetic determinants strongly influence HDL cholesterol (HDLc) levels, most causes underlying variation in HDLc remain unknown. We aimed to identify novel rare mutations with large effects candidate genes contributing extreme humans, utilizing family-based Mendelian genetics. performed next-generation sequencing of 456 HDLc-regulating 200 unrelated probands extremely low (≤10th percentile) or high (≥90th HDLc. Probands were excluded if known existed the established ABCA1, APOA1, LCAT,...
1. The activities of ornithine decarboxylase, S-adenosylmethionine decarboxylase and ornithine-2-oxoglutarate aminotransferase were studied during the first 24 h conidial germination in Aspergillus nidulans. 2. Increases (over 100-fold) occurred emergence germ-tube before doubling DNA this was followed by a sharp fall both enzymes 16h. 3. increase could be largely suppressed if 0.6 mM-putrescine added to growth medium. 4. Low concentrations cycloheximide, which delayed 2h, caused...
The determinant for the K88ac adherence antigen of porcine enterotoxigenic Escherichia coli has been cloned previously onto vector plasmid pBR322 to form K88ac-pBR322 hybrid pMK005 (M. Kehoe et al., Nature [London] 291:122-126). Further studies on expression from are presented in this paper. Expression was found be dependent mainly P1 promoter vector. natural apparently not original parental plasmid. deleted and replaced by a DNA sequence encoding promoter-operator region E. tryptophan (Trp)...