A5050327323- Cellular Mechanics and Interactions
- Pancreatic and Hepatic Oncology Research
- Cancer Immunotherapy and Biomarkers
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Cell Adhesion Molecules Research
- Axon Guidance and Neuronal Signaling
- Malaria Research and Control
- Glycosylation and Glycoproteins Research
- Proteoglycans and glycosaminoglycans research
- Phagocytosis and Immune Regulation
- Immunotherapy and Immune Responses
- Protease and Inhibitor Mechanisms
- Computational Drug Discovery Methods
- Blood properties and coagulation
- Antimicrobial Peptides and Activities
- RNA Interference and Gene Delivery
- Preterm Birth and Chorioamnionitis
- Nerve injury and regeneration
- Neonatal and Maternal Infections
- COVID-19 Impact on Reproduction
- Hemoglobinopathies and Related Disorders
- Cancer Genomics and Diagnostics
- Nuclear Structure and Function
- Cancer Research and Treatments
Indian Institute of Technology Bombay
2014-2025
The University of Texas MD Anderson Cancer Center
2023-2024
Inflammation and tissue damage associated with pancreatitis can precede or occur concurrently pancreatic ductal adenocarcinoma (PDAC). We demonstrate that in PDAC coupled (ptPDAC), antigen-presenting type I conventional dendritic cells (cDC1s) are specifically activated. Immune checkpoint blockade therapy (iCBT) leads to cytotoxic CD8 + T cell activation elimination of ptPDAC restoration life span even upon rechallenge. Using antigen-loaded cDC1s as a vaccine, immunotherapy-resistant was...
Infection of the genitourinary tract with Group B Streptococcus (GBS), an opportunistic gram positive pathogen, is associated premature rupture amniotic membrane and preterm birth. In this work, we demonstrate that GBS produces vesicles (MVs) in a serotype independent manner. These MVs are loaded virulence factors including extracellular matrix degrading proteases pore forming toxins. Mice chorio-decidual membranes challenged ex vivo resulted extensive collagen degradation leading to loss...
Mutations in KRAS are a dominant driver of pancreatic ductal adenocarcinoma (PDAC), with over 40% PDAC patients presenting G12D mutations. The recent development small molecule inhibitors targeting has enabled mutant signaling and suppression PDAC; however, the contribution tumor microenvironment (TME) to sustained therapeutic efficacy inhibition mechanism/s resistance remain be elucidated. Here, we employed spatial transcriptomics, single cell RNA sequencing, CODEX-based proteomics evaluate...
ABSTRACT In addition to proteins such as collagen (Col) and fibronectin, the extracellular matrix (ECM) is enriched with bulky proteoglycan molecules hyaluronic acid (HA). However, how ECM proteoglycans collectively regulate cellular processes has not been adequately explored. Here, we address this question by studying cytoskeletal focal adhesion organization dynamics on cells cultured polyacrylamide hydrogels functionalized Col, HA a combination of Col (Col/HA). We show that fastest...
Differentiation of stem cells into neurogenic lineage is great interest for treatment neurodegenerative diseases. While the role chemical cues in regulating cell fate well appreciated, identification physical has revolutionized field tissue engineering leading to development scaffolds encoding one or more inducing differentiation. In this study, using human mesenchymal (hMSCs) and mouse embryonic (mESCs), we have tested if stiffness topography can be collectively tuned neuronal...
Physical properties of the extracellular matrix (ECM) are known to regulate cellular processes ranging from spreading differentiation, with alterations in cell phenotype closely associated changes physical cells themselves. When plated on substrates varying stiffness, fibroblasts have been shown exhibit stiffness matching property, wherein cortical increases proportion substrate up 5 kPa, and subsequently saturates. Similar mechanoadaptation responses also observed other types. Trypsin...
Malaria is a deadly disease killing worldwide hundreds of thousands people each year and the responsible parasite has acquired resistance to available drug combinations. The four vacuolar plasmepsins (PMs) in Plasmodium falciparum involved hemoglobin (Hb) catabolism represent promising targets combat resistance. High antimalarial activities can be achieved by developing single that would simultaneously target all PMs. We have demonstrated for first time use soluble recombinant plasmepsin II...
Abstract Mutations in KRAS are a dominant driver of pancreatic ductal adenocarcinoma (PDAC), with over 50% PDAC patients presenting KRASG12D mutations. The recent development small molecule inhibitors targeting has enabled effective mutant signaling and suppression tumor growth; however, the contribution microenvironment to therapeutic efficacy inhibition mechanisms resistance remain be fully elucidated. Here, we employed spatial transcriptomics proteomics evaluate cancer cell intrinsic...
Abstract Embryonic stem cells (ESCs) which are susceptible to DNA damage depend on a robust and highly efficient response (DDR) mechanism for their survival. However, the implications of physical force-mediated ESC fate remains unclear. We show that stiffness-dependent spreading mouse ESCs (mESCs) induces through nuclear compression, with causing differentiation early induction Lamin A/C expression. Interestingly, is associated rescue activation DDR factor ATR. While ATR typically known play...
Abstract In addition to proteins such as collagen (Col) and fibronectin, the extracellular matrix (ECM) is enriched with bulky proteoglycan molecules hyaluronic acid (HA). However, how ECM proteoglycans collectively regulate cellular processes has not been adequately explored. Here, we address this question by studying cytoskeletal focal adhesion organization dynamics on cells cultured polyacrylamide hydrogels functionalized Col, HA a combination of Col (Col/HA). We show that fastest...
ABSTRACT Growth cone - mediated axonal outgrowth and accurate synaptic targeting are central to brain morphogenesis. Translocation of the growth necessitates mechanochemical regulation cell extracellular matrix interactions generation propulsive traction forces onto environment. However, molecular mechanisms subserving force by cones remain poorly characterized. The formin family member, Fmn2, has been identified earlier as a regulator motility. Here, we explore underlying Fmn2 function in...