Dextran sodium sulfate (DSS)-induced colitis mouse model is used for research of inflammatory bowel disease. The aim this study was to establish the adequate conditions DSS mice model, and find useful tool measure inflammation.The 2.5% administered six male C57BL/6 4% eight at 5 or 9 weeks age. Each group consisted 6 with control in which vehicle instead DSS. were sacrificed on 7th day after administration. Body weight, diarrhea, hematochezia recorded daily. Disease activity index (DAI)...

This study demonstrates that estradiol downregulates inflammation and inhibits colorectal cancer (CRC) development in azoxymethane/dextran sulfate sodium (AOM/DSS) mouse model.AOM/DSS-treated male female mice were sacrificed at weeks 2, 10, 16, to assess estrogen effects on colitis carcinogenesis. Macroscopic histologic severity of Western blot quantitative real-time polymerase chain reaction evaluated, measure inflammatory mediators cytokines.Compared with AOM/DSS-treated (M-AOM/DSS group),...

Developing a process to generate dendritic cells (DCs) applicable for multicenter trials would facilitate cancer vaccine development. Moreover, targeting multiple antigens with such strategy could enhance the efficacy of treatment approach. We performed phase 1/2 clinical trial administering DC-based tumor-associated patients advanced colorectal (CRC). A qualified manufacturing was used DC from blood monocytes using granulocyte macrophage colony-stimulating factor and IL-13, matured 6 hours...

The colitis-associated cancer exhibits different characteristics according to sex in the initiation and progression of tumors. aim this study was investigate sex-associated difference azoxymethane/dextran sulfate sodium (AOM/DSS)-induced model.The AOM/DSS ICR mouse model established compare male with female, then severity carcinogenesis examined macroscopically histologically regarding number, size, location Subsequently, levels colonic mucosal cytokine, interleukin (IL)-1β myeloperoxidase...

Estrogen is known to have a protective effect in colorectal cancer (CRC) development. Previously, we reported the anti-inflammatory and antitumorigenic effects of 17β-estradiol (E2) azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated male mice. The aim this study was investigate whether ovariectomy female AOM/DSS mouse model increases tumorigenesis reduced by estrogen supplementation after ovariectomy. Clinical symptoms histological severity colitis levels inflammatory mediators were...

ABSTRACT Background The clinical utility of urinary red blood cell (RBC) distribution (URD) remains limited. This study aimed to compare the diagnostic performance URD and dysmorphic RBC (dRBC) in a multicenter study. Methods enrolled 703 patients who visited four tertiary medical centers Korea. Patients were classified into glomerular diseases with biopsy ( N = 169), renal including chronic kidney disease 194), nephrotic syndrome (NS; 88), tubulointerstitial 36), acute injury 32), others...

rdxA and frxA mutations enhancement of efflux pump have been suggested as the cause metronidazole resistance in Helicobacter pylori. This study was performed to investigate mechanisms related clinical eradication outcome, examine direct involvement hefA metronidazole-resistant isolates with intact frxA.A total 53 H. pylori-positive patients who were treated metronidazole-containing sequential or quadruple therapy from 2011 2015 enrolled. The susceptibility pylori examined by agar dilution...

Gut microbiota, an important component that affects host health, change rapidly and directly in response to altered diet composition. Recently, the role of diet-microbiome interaction on development colon cancer has been focus interest. Colon occurs more frequently aged population, males. However, effect dietary changes gut microbiome studied mainly young males, even though it may vary with age sex. The aim this study was investigate microbial colons male female 6-week-old (young) 2-year-old...

Intestinal barrier dysfunction is a hallmark of inflammatory bowel diseases (IBDs) such as ulcerative colitis. This caused by increased permeability and the loss tight junctions in intestinal epithelial cells. The aim this study was to investigate whether estradiol treatment reduces colonic permeability, junction disruption, inflammation an azoxymethane (AOM)/dextran sodium sulfate (DSS) colon cancer mouse model.The effects 17β-estradiol (E2) were evaluated ICR male mice 4 weeks after...

The association between adverse effects of PPI and gut microbiota in old age has yet to be elucidated. We assessed changes the butyrate levels following long-term administration PPIs rats investigated their associations. F344 aged male were fed a PPI-supplemented diet for 50 weeks. ileal was analysed by metagenomic sequencing 16S rRNA, while concentration measured high-performance liquid chromatography. observed significant decrease microbial diversity 2-year-old but not 74-week-old rats....

This study aimed to characterize the changes in gut microbiota of irritable bowel syndrome (IBS) patients and investigate consequent alterations bacterial functions.We performed 16S rRNA metagenomic sequencing a phylogenetic investigation communities by reconstruction unobserved states (PICRUSt) analyses using fecal samples from control (n=12) diarrhea-dominant IBS (n=7).The were clustered principal coordinates analysis depending on presence (p=0.003). In patients, abundances...

The colonic response to stress is greater in female rats than male rats. aim of this study was evaluate the effect probiotics repeated water avoidance (rWAS)-induced microinflammation model Wistar a sex-specific manner. three groups (no-stress, WAS, and WAS with probiotics) were exposed r-WAS for 1 h daily 10 days, Lactobacillus farciminis administered by oral gavage days animals group. visceromotor (VMR) colorectal distension (CRD) assessed using barostat noninvasive manometry before after...

to evaluate gender differences in the aspect of ghrelin, nociception-related genes and psychological aspects quality life (QoL) Korean functional dyspepsia (FD) patients.Total 191 persons were prospectively enrolled between March 2013 May 2016 Seoul National Bundang Hospital, classified into control FD group based on ROME III criteria. Questionnaire included assessment for symptoms, QoL anxiety or depression. Preproghrelin nociception gastric mucosa plasma acyl/des-acyl ghrelin...

: The gut microbiome has emerged as a key player that mechanistically links various risk factors to colorectal cancer (CRC) etiology. However, the role of in CRC pathogenesis remains unclear. This study aimed characterize microbiota healthy controls (HCs) and patients with adenoma (AD) subgroups based on sex age.

Several single center studies have provided evidence of immune activation and antitumor activity therapeutic vaccination with dendritic cells (DC) in patients metastatic melanoma. The efficacy this approach favorable prognosis melanoma limited to the skin, subcutaneous tissues lung (stages IIIc, M1a, M1b) was tested a multicenter two stage phase 2 study centralized DC manufacturing. vaccine (IDD-3) consisted 8 doses autologous monocyte-derived matured generated serum-free medium granulocyte...

Gut microbiota contributes to intestinal and immune homeostasis through host-microbiota interactions. Distribution of the gut differs according location in gastrointestinal tract. Although properties change with age, evidence for regional difference has been restricted young. The aim this study is compare between terminal ileum cecum old rats.We analyzed microbiome luminal contents from 74-week-old 2-year-old rats (corresponding 60-year 80-year-old human age) by metagenome sequencing 16S...

Background/Aims Estrogen is known to have protective effect in colorectal cancer development. The aims of this study are investigate whether estradiol treatment reduces inflammation CCD841CoN, a female human colonic epithelial cell line and uncover underlying mechanisms effects. Methods 17β-Estradiol (E2) was measured by Western blot after inducing inf lammation CCD841CoN tumor necrosis factor α (TNF-α). Expression levels estrogen receptor (ERα) β (ERβ), cyclooxygenase-2 (COX-2),...

Emerging evidence shows that the mechanism of irritable bowel syndrome (IBS) is associated with neurotrophic factors and tight junction proteins (TJPs). It known there are sex differences in pathophysiology IBS. The aim present study to determine expression levels factors, TJPs, cytokines according IBS subtype sex.From 59 (33 IBS-constipation, 21 IBS-diarrhea, 5 IBS-mixed) 36 control patients, colonic mucosa mRNA transient receptor potential vanilloid-1 (TRPV1), nerve growth factor (NGF),...

Background Cerebrotendinous xanthomatosis (CTX) is a rare autosomal recessive lipid storage disease caused by mutation in the CYP27A1 gene. Due to disruption of bile acid synthesis leading cholesterol and cholestanol accumulation, CTX manifests as premature cataracts, chronic diarrhea, intellectual disability childhood adolescence. This report presents case with an unusual phenotype behavioral variant frontotemporal dementia (bvFTD) middle age. Case presentation A 60-year-old woman presented...

Abstract Background and Aim Failure of bismuth quadruple therapy for Helicobacter pylori eradication is frequently observed. To increase the rate, comprehensive analyses need to be performed regarding risk factors failure based on complete standard culture antimicrobial susceptibility testing results. Methods Patients with history failed first who had H. colonies isolated from successful minimum inhibitory concentration (MIC) test were enrolled. Esomeprazole, bismuth, metronidazole,...

Male predominance has been observed in the erosive reflux disease (ERD), but reverse finding nonerosive (NERD). This suggests sex-specific medicine approach is needed its mechanism remained to be elucidated. We aimed compare clinical characteristics and mRNA expression levels of tight junction-related proteins between male female gastroesophageal (GERD).Sixteen healthy controls, 45 ERD, 14 NERD patients received upper endoscopies completed questionnaires. Quantitative real-time polymerase...

Skeletal muscles are made up of various muscle fiber type including slow and fast-twitch fibers. Because each has its own physiological characteristics, the effects aging exercise vary depending on fiber. We used bioinformatics screening techniques such as differentially expressed gene analysis, ontology analysis set enrichment to try understand genetic differences between types. The experiment expression profiling in this study soleus (SOL, slow-twitch muscle) gastrocnemius (GAS, muscle)....

Abstract Background Amoxicillin (Amx) is one of the most important antibiotics for eradication Helicobacter pylori ( H. ). Main determinants genetically stable Amx resistance are mutations in C‐terminus penicillin‐binding protein 1A pbp1A However, contribution individual mutation remains unclear. Methods 77 Amx‐resistant (Amx R ) and Amx‐susceptible S strains were isolated from gastric tissues, DNA sequencing was performed to compare sequences gene between strains. Natural transformation...