A5028561124- Advanced Electron Microscopy Techniques and Applications
- Microbial Community Ecology and Physiology
- Photosynthetic Processes and Mechanisms
- Image Processing Techniques and Applications
- Neurobiology and Insect Physiology Research
- Crustacean biology and ecology
- Cell Image Analysis Techniques
- Protist diversity and phylogeny
- Calcium Carbonate Crystallization and Inhibition
- Electron and X-Ray Spectroscopy Techniques
- Bacteriophages and microbial interactions
- Medical Image Segmentation Techniques
- Bone Metabolism and Diseases
- Enzyme Structure and Function
- Plant-Microbe Interactions and Immunity
- Marine and coastal ecosystems
- Monoclonal and Polyclonal Antibodies Research
- Extracellular vesicles in disease
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Photoacoustic and Ultrasonic Imaging
- Light effects on plants
- Insect symbiosis and bacterial influences
- Caveolin-1 and cellular processes
- Transgenic Plants and Applications
University of Delaware
2011-2023
Biotechnology Institute
2012-2023
Fraunhofer USA Center for Molecular Biotechnology
2011-2018
Delaware Technical Community College
2014
Imaging Center
2011
University of North Carolina Wilmington
2009
Washington College
2002
Abstract Plasmodesmata (PD) are thought to play a fundamental role in almost every aspect of plant life, including normal growth, physiology, and developmental responses. However, how specific signaling pathways integrate PD-mediated cell-to-cell communication is not well understood. Here, we present experimental evidence showing that the Arabidopsis thaliana plasmodesmata-located protein 5 (PDLP5; also known as HOPW1-1-INDUCED GENE1) mediates crosstalk between PD regulation salicylic...
Osteocytes, the most abundant bone cells, form an interconnected network in lacunar-canalicular pore system (LCS) buried within mineralized matrix, which allows osteocytes to obtain nutrients from blood supply, sense external mechanical signals, and communicate among themselves with other cells on surfaces. In this study, we examined key features of LCS including topological parameter detailed structure individual connections their variations cortical cancellous compartments, at different...
Dynamic tubular extensions from chloroplasts called stromules have recently been shown to connect with nuclei and function during innate immunity. We demonstrate that extend along microtubules (MTs) MT organization directly affects stromule dynamics since stabilization of MTs chemically or genetically increases numbers length. Although actin filaments (AFs) are not required for extension, they provide anchor points stromules. Interestingly, there is a strong correlation between the direction...
Abstract Osteocytes project long, slender processes throughout the mineralized matrix of bone, where they connect and communicate with effector cells. The interconnected cellular projections form functional lacunocanalicular system, allowing fluid to pass for cell-to-cell communication nutrient waste exchange. Prevention mineralization in pericellular space is crucial uninhibited interstitial movement. Factors contributing ability system remain open unmineralized are unclear....
We developed an approach for focused gallium-ion beam scanning electron microscopy with energy filtered detection of backscattered electrons to create near isometric voxels high-resolution whole cell visualization. Specifically, this method allowed us three-dimensional volumes high-pressure frozen, freeze-substituted Saccharomyces cerevisiae yeast cells pixel resolutions down 3 nm/pixel in x, y, and z, supported by both empirical data Monte Carlo simulations. As a result, we were able...
We report that two different bacterial organisms engage in heterologous cell fusion leads to massive exchange of cellular material, including proteins and RNA, the formation persistent hybrid cells. The interspecies observed here involves a syntrophic microbial system, but these fusions were even under nonstrict conditions, leaving open possibility strict syntrophy may not be necessary for material exchange. Formation cells contain RNA from both is unexpected unprecedented. Such events are...
Elemental sulfur (S0) is produced and degraded by a diverse group of microorganisms. For Chlorobaculum tepidum, an anoxygenic phototroph, sulfide oxidized to produce extracellular S0 globules, which can be further sulfate. While some sulfur-oxidizing bacteria (e.g. Al. vinosum) are also capable growth on commercial as electron donor, Cba. tepidum not. Even colloidal sols, appear indistinguishable from biogenic do not support the tepidum. Here, we investigate properties that make globules...
In this study, we have developed a robust cryohistological method that allows imaging of virtually any type plant cell or tissue while preserving fluorescent protein signals and maintaining excellent cellular subcellular morphology. This involves modified fixation tissues (i.e., leaves, stems, petioles), infiltration in sucrose gradient, freezing, collection cryosections directly onto cryoadhesive tape. Using followed by microscopic analysis, demonstrated localized accumulation green (GFP)...
We examined the three-dimensional organization of endothelial vesicular system with TEM tomography semi-thick sections.Mouse abdominal muscle capillaries were perfused terbium to label compartments open luminal surface. The tissue was prepared for and (250 nm) sections cut. Dual axis tilt series, collected from +60° -60° at 1° increments, acquired in regions labeled abluminal caveolae. These tomograms reconstructed analyzed reveal associations not evident thin sections.Reconstructed revealed...
Crustaceans must replace their old exoskeleton with a new, larger one in order to grow and differentiate. During that process new (pre-exuvial) cuticle is deposited beneath the cuticle. Since breakdown products from have been reported be removed transported across hypodermis into hemolymph, preexuvial presumed permeable. However, it has also intermolt highly impermeable structure prevents loss of water ions external environment. This study was designed determine timing any change...
Protein precipitates that arise during bioprocessing can cause manufacturing challenges, but they also aid in clearance of host-cell protein (HCP) and DNA impurities. Such differ from many have been studied previously their heterogeneous composition, particularly the presence high concentrations product protein. Here, we characterize form after neutralization A purified viral-inactivated material an Fc-fusion produced Chinese hamster ovary cells. The physical growth precipitate particles was...
Marek's disease (MD) is an alphaherpesvirus (Marek's virus, MDV)-induced pathology of chickens associated with paralysis, immunosuppression, neurological signs, and T-cell lymphomas. MD controlled in poultry production via live attenuated vaccines. The purpose the current study was to compare methods for precipitating exosomes from vaccinated protected chicken sera (VEX) tumor-bearing (TEX) biomarker analysis vaccine-induced protection lymphomas respectively. A standard polyethylene glycol...