A5058744784- Connexins and lens biology
- Inflammatory mediators and NSAID effects
- Cancer, Hypoxia, and Metabolism
- Intraocular Surgery and Lenses
- Cancer, Stress, Anesthesia, and Immune Response
- Prostate Cancer Treatment and Research
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Eicosanoids and Hypertension Pharmacology
- Ocular Surface and Contact Lens
- Immune cells in cancer
- Prostate Cancer Diagnosis and Treatment
- RNA Research and Splicing
- Heat shock proteins research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- HIV Research and Treatment
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Aldose Reductase and Taurine
- Corneal Surgery and Treatments
- Congenital gastrointestinal and neural anomalies
- T-cell and Retrovirus Studies
- Advanced X-ray Imaging Techniques
- Plant Virus Research Studies
Frederick National Laboratory for Cancer Research
2017-2025
Leidos (United States)
2017-2022
National Cancer Institute
2017-2022
National Institutes of Health
2017-2021
University of Delaware
2010-2016
Biotechnology Institute
2012
Significance Nitric oxide synthase-2 (NOS2) and cyclooxygenase-2 (COX2) are inflammation-associated enzymes with oncogenic function in breast cancer. We show that crosstalk between NOS2/COX2 promotes aggressive phenotypes elevated coexpression of tumors predict significantly reduced patient survival (33%) when compared 95% estrogen receptor-negative patients low tumor expression. In addition, we have identified a subtype-specific mechanism showing involvement TNFα and/or endoplasmic...
Abstract Posterior capsular opacification ( PCO ) is the major complication arising after cataract treatment. occurs when lens epithelial cells remaining following surgery LC s) undergo a wound healing response producing mixture of α‐smooth muscle actin (α‐ SMA )‐expressing myofibroblasts and fibre cells, which impair vision. Prior investigations have proposed that integrins play central role in we found that, mouse cell removal model surgery, expression α V integrin its interacting...
Radiotherapy enhances innate and adaptive anti-tumour immunity. It is unclear whether this effect may be harnessed by combining immunotherapy with radiotherapy fractions used to treat prostate cancer. We investigated tumour immune microenvironment responses of pre-clinical cancer models radiotherapy. Having defined landscape, we tested radiotherapy-induced growth delay could enhanced anti-PD-L1.
The role of nitric oxide (NO) in cancer progression has largely been studied the context tumor NOS2 expression. However, pro- versus anti-tumor signaling is also affected by cell-macrophage interactions. While these cell-cell interactions are partly regulated NO, functional effects NO flux on proinflammatory (M1) macrophages unknown. Using a triple negative murine breast model, we explored potential macrophage Nos2 4T1 progression. phenotype were examined bone marrow derived from wild type...
Background: Multiplexed tissue imaging enables the simultaneous detection of dozens proteins at single-cell resolution, providing unprecedented insights into organization and disease microenvironments. However, resulting high-dimensional, gigabyte-scale datasets pose significant computational methodological challenges. Existing analytical workflows, often fragmented between bespoke scripts static visualizations, lack scalability user-friendly interfaces required for efficient, reproducible...
Abstract Lamin A/C (LMNA), a key component of the nuclear envelope, plays an essential role in maintaining integrity and regulating genome organization. While LMNA dysregulation has been linked to genomic instability both cancer aging, specific mechanisms remain poorly understood. We investigated small cell lung (SCLC), highly lethal form characterized by neuroendocrine (NE) differentiation, low expression, extreme instability. show that depletion leads increased R-loop formation,...
Antitumor immune polarization is a key predictor of clinical outcomes to cancer therapy. An emerging concept influencing outcome involves the spatial location CD8
In this study, we have developed a robust cryohistological method that allows imaging of virtually any type plant cell or tissue while preserving fluorescent protein signals and maintaining excellent cellular subcellular morphology. This involves modified fixation tissues (i.e., leaves, stems, petioles), infiltration in sucrose gradient, freezing, collection cryosections directly onto cryoadhesive tape. Using followed by microscopic analysis, demonstrated localized accumulation green (GFP)...
Abstract The tumor microenvironment (TME) is multi-cellular, spatially heterogenous, and contains cell-generated gradients of soluble molecules. Current cell-based model systems lack this complexity or are difficult to interrogate microscopically. We present a 2D live-cell chamber that approximates the TME demonstrate breast cancer cells macrophages generate hypoxic nutrient gradients, self-organize, have varying phenotypes along leading new insights into tumorigenesis.
There is a need to improve the treatment of prostate cancer (PCa) and reduce side effects. Vascular-targeted photodynamic therapy (VTP) focal for low-risk low-volume localised PCa, which rapidly disrupts targeted tumour vessels. interest in expanding use VTP higher-risk disease. Tumour vasculature characterised by vessel immaturity, increased permeability, aberrant branching inefficient flow. FRT alters microenvironment promotes transient 'vascular normalisation'. We hypothesised that...
The MT-4 human T-cell line expresses HTLV-1 Tax and is permissive for replication of an HIV-1 gp41 mutant lacking the cytoplasmic tail. cells (lot 150048), distributed by NIH AIDS Reagent Program (NIH-ARP), were found to be deficient unable host gp41-truncated mutant. These findings, together with short tandem repeat profiling, established that lot 150048 are not bona fide cells.
The HIV-1 envelope glycoprotein (Env) contains a long cytoplasmic tail harboring highly conserved motifs that direct Env trafficking and incorporation into virions promote efficient virus spread. cellular factor Rab11a family interacting protein 1C (FIP1C) has been implicated in the directed of to sites viral assembly. In this study, we confirm small interfering RNA (siRNA)-mediated depletion FIP1C HeLa cells modestly reduces virions. To determine whether is required for replication...
Abstract Anti-tumor immune polarization is a key predictor of clinical outcomes to cancer therapy. An emerging concept influencing outcome involves the spatial location CD8 + T cells, within tumor. Our earlier work demonstrated immunosuppressive effects NOS2/ COX2 tumor expression. Here, we show that NOS2/COX2 levels influence and lymphoid cells including cells. Importantly, elevated correlated with exclusion from epithelium. In contrast, tumors expressing low had increased cell penetration...
We study the molecular mechanisms that are essential for development and maintenance of transparency in eye lens. Toward this goal, we have applied bioinformatics-based gene discovery tool ...