A5029289026- Glioma Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Cancer Research and Treatments
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- TGF-β signaling in diseases
- RNA Interference and Gene Delivery
- Cancer, Hypoxia, and Metabolism
- Neuroblastoma Research and Treatments
- Glycosylation and Glycoproteins Research
- Renal and related cancers
- Circular RNAs in diseases
- Cancer Immunotherapy and Biomarkers
- Cancer therapeutics and mechanisms
- Ferroptosis and cancer prognosis
- Medicinal Plant Pharmacodynamics Research
- Drug Transport and Resistance Mechanisms
- Pregnancy and preeclampsia studies
- Medical Imaging and Analysis
- Retinoids in leukemia and cellular processes
- Microbial Metabolism and Applications
- Cancer Mechanisms and Therapy
- Signaling Pathways in Disease
- Photodynamic Therapy Research Studies
- Peptidase Inhibition and Analysis
Wuhan University
2013-2024
Renmin Hospital of Wuhan University
2012-2024
Children's Hospital of Fudan University
2023
Fudan University
2022
Abstract Background Glioma is the most common primary brain tumor and represents one of aggressive lethal types human cancer. BCL7 family has been found in several cancer could be involved progression. While role glioma remained to elucidated. Methods Paraffin-embedded samples were obtained detect expression by performing glioma. Data (including normalized gene corresponding clinical data) from Gliovis, CGGA, GEO, cBioportal Oncomine used investigate genes Survival analyses calculated...
Gliomas are commonly malignant tumors that arise in the human central nervous system and have a low overall five-year survival rate. Previous studies reported several members of Rab GTPase family involved development glioma, abnormal expression small GTPases is known to cause aberrant tumor cell behavior. In this study, we characterized roles Rab21 (Rab 21), member family, glioma cells.The study downregulation two lines (T98G U87) through transfection with specific-siRNA. Experiments using...
Purpose: Cancer stem cells have recently been thought to be closely related tumor development and reoccurrence.It may a promising way cure malignant glioma by using cell-targeted dendritic as vaccine.In this study, we explored whether pulsing with antigens of was potent induce specific cytotoxic T lymphocytes anti-tumor immunity.Materials Methods: were cultured from cell line U251.Lysate obtained the repeated freezing thawing method.Dendritic (DCs) induced murine bone marrow cells,...
Introduction: Functional disorder of the placenta is principal cause fetal growth restriction (FGR), usually cured with suitable clinical treatment and good nursing. However, some FGR mothers still give birth to small for gestational age (SGA) babies after treatment. The ineffectiveness in such a group patients confused physicians obstetrics gynecology. Methods: In this study, we performed microRNA-messenger RNA integrative analysis gene expression profiles obtained from Gene Expression...
Glioma is the most common form of primary malignant intracranial tumor.In current study, miRNA matrix were obtained from Chinese Genome Atlas (CGGA), and then univariate Cox regression analysis Lasso utilized to select candidate miRNAs multivariate was applied establish a signature for predicting overall survival (OS) glioma. The assessed with area under curve (AUC) receiver operating characteristic (ROC) validated by data Gene Expression Omnibus (GEO).Eight (miR-1246, miR-148a, miR-150,...
This study was aimed at investigating the mechanism of PATZ1 inducing apoptosis through PUMA in glioblastoma. Overexpressed transfected to explore its role glioblastoma cells.The expression protein detected by western blotting assay. qRT-PCR assay used detect RNA. Confocal microscopy analyze correlation between and PUMA. TUNEL cell apoptosis. The ability proliferation MTT EDU effects on tumor were observed vivo xenograft mouse model.The results showed that low correlates with poor prognosis...
Objective To investigate the effect of MS-275 on proliferation brain glioma cells. Methods U251 cells were respectively cultured for 24, 48, 72 h, with different concentrations MS-275. CCK-8 assay was used to assess The morphological changes observed by Wright-Giemsa staining. cell cycle analyzed PI dyeing. apoptosis rate assayed flow cytometry using annexin V-FITC/PI. protein expression STAT3 and PARP detected Western blotting. Results could significantly inhibited After treatment MS-275,...
Objective To investigate the role of leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) in U251 cells its gene changes.Methods The stable cell line over-expressed LRIG1 (PEGFP-LRIG1) was established; PEGFP-N1-U251 blank-U251 were used as controls.The expression changes ofLRIG1,GDNF topoisomerase Ⅱ (TOPOⅡ) detected by real time-PCR; Western blotting employed to detect protein expression; growth curve PEGFP-LRIG1-U251 drew from 1st 5th d,and inhibition rate temozolomide these two...
Objective To investigate the effects of signal transducers and activators transcription 3 (STAT3) signaling pathway on leucine-rich repeats immunoglobulin-like domains 1 (LRIG1)induced apoptosis U251 cells mechansim.Methods were cultured randomly divided into three groups:control group,N1-U251 LRIG1-U251,which respectively transfected with PBS,PEGFP-N1 plasmid PEGFP-LRIG1 by liposome transfection.Cell counting kit-8 (CCK-8) assay was used to assess proliferation cells,and rate flow cytometry...
Objective The multidrug resistant cell line U251/VP16 was established and induced by etoposide.Methods U251 cells to etoposide were make increasing concentrations.The resistance detected counting Kit-8 (CCK-8).The morphology of observed after Wright-Giemsa staining.Cell cycle tested flow cytometry.Cell doubling time calculated.The expression ( MDR1 ),B lymphocytes/leukemia-2(bcl-2),multidrug associated protein 5 (MRP5) low density lipoprotein receptor related 1 (LRP1) assayed using reverse...
Objective To investigate the gene therapy of experimental rat intracranial glioma by MSCs-hIFN-β. Methods Cellular viability C6 cell was assessed MTT assay and content hIFN-β analyzed using enzyme linked immunosorbent (ELISA). model established verified histopathological characteristics MRI images. Clinical manifestation bearing were recorded. Direct intratumoral injection MSCs-IFN-β undertaken, survival mices The expression protein in tissure examined immunohistochemically. Results MSCs...
Objective To explore the ability of anti-glioma cells and mechanism specific T induced by dendritic loaded with antigen glioma stem cells.Methods The cancer were cultured from U251 cell line.The lysate was obtained through repeated freezing thawing method.Dendritic (DCs) prepared mouse mesenchymal cells.The vaccine produced mixing tumor DCs.Then,the surface markers DCs checked flowcytometry.CCK-8 method used to detect DC vaccine' s promoting proliferation killing level IFN-γ in supernatant...
Objective To investigate the effect of expression leucine-rich repeats and immunoglobulin-like domains 1 （LRIG1） on radiosensitivity human glioma cell line U251 cells possible mechanism. Methods The PEGFP-N1, PEGFP-LRIG1 plasmids were transfected into with application liposome-mediated gene transfer technology. And stably selected by G418. N1-U251 LRIG1-U251 was analyzed clonogenic assay. Western blotting demonstrated LRIG1 RAD51. Repairment double-strand （ds） DNA breaks measured comet...
Objective To investigate the relationship of bone morhogenetic proteins 4 (BMP4)and chemotherapy sensitivity in glioma.Methods The tissue microarry (TMA) human brain astrocytoma was constructed and analyzed for expresion BMP4,B lymphocytes/leukemia-2 (bcl-2) MnSOD by using SP immunohistochemistry.Temozolomide (TMZ) resistant cell line expression BMP4 detected.BMP4 plasmid transfected into TMZ cells,and tested.Results average absorbance ( WHO Ⅰ:0.3320 ± 0.0065,WHO Ⅱ:O.2485 ±0.0066,WHOⅢ:0.1701...
Objective To investigate the ability and mechanism of dendritic cells (DCs) inducing specific cytotoxicity T to kill giloma cells.Methods DCs were induced cultured from murine bone marrow by using modified Inaba method in vitro.The biological characteristics detected an electron microscope flow cytometry.The vaccine was obtained mixing with apoptotic glioma heating treatment.The capacity promoting proliferation killing tested cell counting kit-8 (CCK-8).DCs injected into tumor-bearing mice...