A5050699602- Gut microbiota and health
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Growth Hormone and Insulin-like Growth Factors
- Cytokine Signaling Pathways and Interactions
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Diabetes and associated disorders
- Rheumatoid Arthritis Research and Therapies
- Alzheimer's disease research and treatments
- Systemic Lupus Erythematosus Research
- Immunodeficiency and Autoimmune Disorders
- Bladder and Urothelial Cancer Treatments
- Monoclonal and Polyclonal Antibodies Research
- Diet and metabolism studies
- Tryptophan and brain disorders
- Probiotics and Fermented Foods
- Psoriasis: Treatment and Pathogenesis
- Extracellular vesicles in disease
- Esophageal Cancer Research and Treatment
- Nerve injury and regeneration
- Chemokine receptors and signaling
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Clostridium difficile and Clostridium perfringens research
- Thermal Regulation in Medicine
Brigham and Women's Hospital
2021-2024
Harvard University
2021-2024
University of Gothenburg
2015-2024
Chalmers University of Technology
2022
Objective This study was undertaken to investigate the gut microbiome in progressive multiple sclerosis (MS) and how it relates clinical disease. Methods We sequenced microbiota from healthy controls relapsing–remitting MS (RRMS) patients correlated levels of bacteria with features disease, including Expanded Disability Status Scale (EDSS), quality life, brain magnetic resonance imaging lesions/atrophy. colonized mice MS‐derived Akkermansia induced experimental autoimmune encephalomyelitis...
Abstract The gut microbiota and microglia play critical roles in Alzheimer’s disease (AD), elevated Bacteroides is correlated with cerebrospinal fluid amyloid-β (Aβ) tau levels AD. We hypothesize that contributes to AD by modulating microglia. Here we show administering fragilis APP/PS1-21 mice increases Aβ plaques females, modulates cortical amyloid processing gene expression, down regulates phagocytosis protein degradation microglial expression. further aged wild-type male female...
Vertically oriented graphene (VG) has attracted attention for years, but the growth mechanism is still not fully revealed. The electric field may play a role, direct evidence and exactly what role it plays remains unclear. Here, we conduct systematic study find that in plasma-enhanced chemical vapor deposition, VG preferably occurs at spots where local stronger, example, GaN nanowire tips. On almost round-shaped nanoparticles, instead of being perpendicular to substrate, grows along...
Abstract The gut microbiota plays crucial roles in maintaining the health and homeostasis of its host throughout lifespan, including through ability to impact brain function regulate behaviour during ageing. Studies have shown that there are disparate rates biologic ageing despite equivalencies chronologic age, development neurodegenerative diseases, which suggests environmental factors may play an important role determining outcomes Recent evidence demonstrates be a potential novel target...
Significance Aberrant insulin-like growth factor 1 receptor (IGF1R)/insulin signaling in brain has recently been linked to neurodegeneration diabetes mellitus and Alzheimer’s disease. In this study, we demonstrate that functional disability pain patients with rheumatoid arthritis (RA) experimental RA are associated hippocampal inflammation inhibition of IGF1R/insulin substrate (IRS1) signal, reproducing an IGF1/insulin-resistant state. This restricts formation new neurons the hippocampus,...
CD8+ T cells have an emerging role in RA. Resent research indicates a causal relationship between the non-exhausted state of cells, defined by lost function PD-1, and development arthritis. We investigated how smoking contributes to phenotype cause survivin release serum. compared serum levels smokers non-smokers 252 RA 168 healthy subjects. Nicotine effects on were studied peripheral blood women, bone marrow nicotine treated mice sorted CD8 spleen vitro using flow cytometry quantitative...
Since low insulin-like growth factor (IGF) 1 is often linked to inflammation, we analyze whether serum levels of IGF1 are associated with cardiovascular disease (CVD) in rheumatoid arthritis (RA) a longitudinal observational study. A CVD risk was estimated (eCVR) 184 female RA patients (mean age 52 years) and 132 after ischemic stroke 56 no rheumatic disease, using the Framingham algorithm. The median level divided cohorts IGF1high IGF1low groups. 5-year prospective follow-up for new events...
The pathophysiology behind radiation cystitis is poorly understood. Here we investigated whether bladder irradiation affects the immune system of rat urinary bladder. Female rats were sedated and exposed to one single dose 20 Gy or only (controls) killed 16 h 14 days later. Rats placed in a metabolic cage at h, 3 days, 7 following irradiation. bladders harvested analysed with qPCR, immunohistochemistry and/or Western blot for expression interferon (IFN)-γ, interleukin (IL)-1β, IL-2, IL-4,...
Smoking is a risk factor for developing rheumatoid arthritis (RA), but the mechanism remains uncertain. We previously demonstrated that smoking lowers T cell activation threshold by limiting programmed death protein 1 (PD-1) expression. To investigate how influence levels of soluble PD-1 ligand (sPD-L1). Serum sPD-L1 were measured in 246 RA patients and 168 healthy subjects. The analysis was done with respect to inflammation, smoking, treatments, autoantibody status. effect therapeutic...
Significance The protein kinase fms-like tyrosine 3 receptor (Flt3) has an important role during early B-cell development. Recent reports have shown that Flt3 is reexpressed on peripheral B cells activation, however the function for activation poorly understood. present study describes a and antibody production. We show activated germinal center lack of functional signaling in results specific deficiency production subclass IgG1. Mechanistic studies indicate synergizes with IL-4 to promote...
Objective: Smoking suppresses PD-1 expression in patients with rheumatoid arthritis (RA). In this study, we assess if smoking changed the epigenetic control over CD8+ T cell memory formation through a microRNA (miR) dependent mechanism. Methods: Phenotypes of cells from smokers and non-smokers, RA healthy, were analyzed by flow cytometry. A microarray analysis was used to screen for differences miR expression. Sorted vitro stimulated nicotine transcription miRs genes related programming...
Objective Insulin-like growth factor 1 receptor (IGF1R) acts at the crossroad between immunity and cancer, being an attractive therapeutic target in these areas. IGF1R is broadly expressed by antigen-presenting cells (APC). Using mice immunised with methylated albumin from bovine serum (BSA-immunised mice) human CD14 + APCs, we investigated role that plays during adaptive immune responses. Methods The mBSA-immunised were treated synthetic inhibitor NT157 or short hairpin RNA to inhibit...
We wanted to assess whether β-adrenoceptors mediate proliferation in the normal and malignant urothelial cell lines UROtsa T24, respectively. Urothelial cells were cultured for 24 h presence of β-adrenoceptor agonists isoprenaline (β1/2/3 ), dobutamine (β1 salbutamol (β2 BRL 37344 (β3 CGP 12177 (a partial β-agonist) or antagonists (metoprolol; β1 , propranolol; β1/2 ). Phosphorylation kinases was screened with a Human Phospho-Kinase Array Kit (R&D systems). Intracellular pathways activated...
Epidermal growth factor receptor (EGFr)-targeted therapy may be used in subgroups of patients with urinary bladder cancer. Here we assessed the role EGFr urothelial proliferation and migration a two- three-dimensional cell culture system.UROtsa cells derived from normal urothelium malignant T24 were cultured Type I collagen gel. Proliferation cells, absence presence inhibitor cetuximab, test (ATCC) Axioplan 2 imaging microscope motorized stage (Carl Zeiss), respectively. The expressions...
<h3>Background:</h3> Neurological involvement in rheumatoid arthritis (RA) is understudied despite a high prevalence of depression, cognitive deficits, chronic pain, and fatigue reported RA patients. Furthermore, there increasing interest the role peripheral brain-resident innate immune cells aging neurodegenerative disease. Systemic activation has been shown to activate microglia affect brain region size models [1,2], but how inflammation affects human not previously studied....
Cosmic radiation experienced during space travel may increase the risk of cognitive impairment. While simulated galactic cosmic (GCRsim) has led to memory deficits in wildtype (WT) mice, it not been investigated whether GCRsim combination with genetic factors for Alzheimer's disease (AD) worsens further aging mice. Here, we central nervous system (CNS) effects 0 Gy (sham) or 0.75 five-ion 2 gamma (IRR) 14-month-old female and male APP
Background: Depression and cognitive impairment have been frequently reported in rheumatoid arthritis (RA) (1). Studies of the molecular mechanisms behind these phenomena attract increasing attention. We previously that signaling through insulin-like receptor is impaired RA has consequences for pain processing (2). Objectives: investigated central peripheral footprint major neurotrophin nervous system, brain-derived neurotrophic factor (BDNF), on mood perception patients. Methods: Pain...
<h3>Background</h3> Smoking is the most extensively studied environmental risk factor of rheumatoid arthritis (RA). We have recently shown that smoking associated with high serum levels survivin (Svensson, Hafström et al. 2014). Survivin a novel biomarker for RA can predict severe joint destruction as well treatment outcome patients (Levitski al, 2015). hypothesized cytotoxic activity to be reason increased observed in and we wished further investigate possible molecular mechanisms behind...
<h3>Background</h3> Rheumatoid arthritis (RA) has recently been linked to an exhausted state of CD4<sup>+</sup> T cells in peripheral blood patients [1]. Exhaustion limits their proliferation and increases cell death. In CD8<sup>+</sup> smoking counteract exhaustion, which may lead increased cytotoxic activity exemplified by targeting with high expression the anti-apoptotic protein survivin [2]. coincides interferon (IFN) response genes [3], referred as IFN signature. The development...