A5038359701- Organ Transplantation Techniques and Outcomes
- Renal Transplantation Outcomes and Treatments
- Fatty Acid Research and Health
- Erythrocyte Function and Pathophysiology
- Dialysis and Renal Disease Management
- Biomedical Research and Pathophysiology
- Hormonal Regulation and Hypertension
- Advanced Glycation End Products research
- Organ Donation and Transplantation
- Neonatal Health and Biochemistry
- Birth, Development, and Health
- Biochemical effects in animals
- Acute Kidney Injury Research
- Liver Disease and Transplantation
- Ion Transport and Channel Regulation
- Renal function and acid-base balance
- Electrolyte and hormonal disorders
- Fibroblast Growth Factor Research
- Pharmacological Effects and Toxicity Studies
- Neurological Disorders and Treatments
- Adrenal and Paraganglionic Tumors
- Renin-Angiotensin System Studies
- Pregnancy and preeclampsia studies
- Adipokines, Inflammation, and Metabolic Diseases
- Sodium Intake and Health
Wroclaw Medical University
2023-2024
Pomeranian Medical University
2010-2022
University of Wrocław
2003-2018
Bernard (Czechia)
2005
FGF1 and FGF2 bind to specific cell-surface tyrosine kinase receptors (FGFRs) activate intracellular signaling that leads proliferation, migration or differentiation of many cell types. Besides this classical mode action, under stress conditions, are translocated in a receptor-dependent manner via the endosomal membrane into cytosol nucleus cell. However, despite years research, role inside remains unclear. Here, we reveal an anti-apoptotic activity FGF2, which is independent FGFR activation...
Renal ischemia-reperfusion (I-R) injury (IRI) is an inseparable feature of organ transplantation and may have a negative impact on the graft, its function survival. Acute tubular necrosis, which reversible thanks to regenerative capacity renal epithelial cells, main cause acute failure secondary IRI. MMP-2 MMP-9 are proteolytic enzymes involved in digesting proteins that components extracellular matrix (ECM) basement membrane nephrons. This way post-reperfusion MMP activation allows...
Abstract Prostaglandin E 2 produced endogenously (by cyclooxygenases) can regulate macrophage phagocytosis. Cyclooxygenase activity reduction (mainly through inhibition of inducible Cox‐2) induce PGE synthesis depression and activate the phagocytosis process. There are no reports in literature explaining whether conjugated linoleic acid dienes ( trans‐ 10, cis ‐12 CLA ‐9, 11 CLA) modify phagocytic human macrophages. For purpose this study, monocytes were isolated from venous blood, incubated...
Dysfunction of endothelial cells and activation monocytes in the vascular wall are important pathogenetic factors atherosclerosis. Conjugated linoleic acids (CLAs) can modulate function immune system humans: reduce concentration atherogenic lipoproteins, intensity inflammatory processes plasma. In this paper, we focus on macrophage's surface integrins (β1 integrin CD49d/CD29-(VLA4); Mac-1 as well human vein cell (HUVEC) adhesins: adhesion molecule-1 (VCAM-1) intracellular (ICAM-1))...
Extracellular fibroblast growth factor 1 (FGF1) acts through cell surface tyrosine kinase receptors, but FGF1 can also act directly in the nucleus, as a result of nuclear import endogenously produced, non-secreted or by transport extracellular via endosomes and cytosol into nucleus. In be phosphorylated protein C δ (PKCδ), this event induces export FGF1. To identify intracellular targets we performed affinity pull-down assays identified nucleolin, multifunctional protein, an interaction...
Apart from their main role in transporting oxygen and carbon dioxide, erythrocytes play also an important organism antioxidative defence. Direct exposure to reactive species (ROS) results shortening of half-life, even by 50%. The presence glucose, being the substrate pentose phosphate pathway (PPP) cycle, is one factors that can have influence on level oxidative stress. activity PPP increases during Glucose guarantees normal functioning with production reductive equivalents amounts necessary...
Abstract Besides its classical mode of action through activation specific receptors at the cell surface, fibroblast growth factor 1 (FGF1) can also cross cellular membrane and translocate into cytosol further to nucleus. The mechanism this translocation is described partially, but role FGF1 inside remains unknown. aim our work was identify novel binding partners predict intracellular functions. We combined three methods identification such based on different principles: yeast two‐hybrid...
Ischaemia-reperfusion injury (IRI) is a factor leading to the damages of transplanted kidney, what affects mainly proximal tubules. Early monitoring tubule damage can be an efficient tool predict allograft dysfunction. Present in tubules, N-acetyl-beta-glucosaminidase (NAG) lysosomal enzyme whose excretion rises as result IRI or acute rejection. The aim this study was monitor NAG urine activity evaluate early damage, and try long-term function kidney.The enrolled 87 Caucasian renal...
ABSTRACT Background The natural history of abdominal aortic aneurysm (AAA) is wall remodelling potentially leading to a final rupture. pathogenesis AAA appears be multifactorial. aim this pilot prospective study was assess the relationship between thickness thrombus within aneurysm, intramural pH and local elastin degradation. Materials methods size, intraluminal (ILT) morphology location were evaluated in 206 consecutive patients. Thirty patients with large (aortic diameter ≥ 50 mm)...
Adsorption of hen egg-white lysozyme (HEWL) molecules at negatively charged polystyrene microparticles was studied using dynamic light scattering (DLS), electrophoresis (LDV), solution depletion techniques, enzyme-linked immunosorbent assay (ELISA), and atomic force microscopy (AFM). The measurements were carried out pHs 3.5 7.4 for NaCl/PBS concentration 10–2 0.15 M. Initially, the dependence electrophoretic mobility zeta potential on bulk determined. These results interpreted in terms...
To analyse the impact of most commonly used immunosuppressive drugs on occurrence apoptosis in native kidneys Wistar rats.The study involved 36 rats. animals being grouped according to regimen (tacrolimus, mycophenolate mofetil, cyclosporine A, rapamycin and prednisone). The rats all groups were treated with a 3-drug protocol for 6 months. medication dose was adjusted based available literature data. No administered control group. then killed. Autopsies performed isolated histopathology (HE...
<i>Background/Aims:</i> The aim of this study was to assess the multifaceted influence glucose present in dialyzing fluid on erythrocytes patients with chronic renal failure (CRF) undergoing regular hemodialysis. <i>Methods:</i> A group 44 subjects CRF hemodialysis studied. Two tests were used: osmotic fragility and resistance hemolytic agent saponin. total content isoprostane 8-iso-prostaglandin F<sub>2</sub>α type III (8-iPF<sub>2</sub>α-III)...
The aim of this study was to assess calcium-phosphate metabolism autosomal dominant polycystic kidney disease (ADPKD) patients with a special consideration the following serum parameters: calcium (Ca(2+)), inorganic phosphate (Pi), parathyroid hormone (PTH) and intracellular erythrocyte ([Ca(2+)]i) concentrations.The included 49 adult ADPKD (19 males, 30 females) aged 36 ±11 years normal renal function no diagnosis diabetes as well 50 healthy controls (22 28 matched for age gender. Serum...
Post-transplant erythrocytosis (PTE) is estimated at 5-20%. Angiotensin II generated by angiotensin I-converting enzyme (ACE) stimulates erythropoiesis. The highest activity of ACE observed in DD genotype. question arises if gene polymorphism influences PTE.One-year prospective study included 89 kidney recipients (28 women and 61 men).Thirty-four (38%) proved to be genotype, 36 (40%) ID, 19 (22%) Absolute PTE [hematocrit level (HCT) >50% RBC > 5.5 mln/mm(3)] was found 14 (19%) patients....
Lipid metabolism disorders are found in patients with chronic renal failure (CRF). Changes the content of fatty acids phospholipid fraction erythrocyte membranes can lead to changes rheological properties. The objective our study was assess effect hemodialysis on composition two fractions phospholipids: sphingomyelin (SPH, representative external monolayer) and phosphatidylethanolamine (PE, internal monolayer). Venous blood drawn from patient CRF before after HD procedure. Lipids stroma were...
Abstract: The structural proteins of renal tubular epithelial cells may become a target for the toxic metabolites immunosuppressants. These can modify properties proteins, thereby affecting cell function, which is possible explanation mechanism immunosuppressive agents' toxicity. In our study, we evaluated effect two strategies on protein expression in kidneys Wistar rats. Fragments rat were homogenized after cooling liquid nitrogen and then dissolved lysis buffer. concentration samples was...