A5029127339- Ubiquitin and proteasome pathways
- interferon and immune responses
- NF-κB Signaling Pathways
- Inflammasome and immune disorders
- Autophagy in Disease and Therapy
- Wnt/β-catenin signaling in development and cancer
- Peptidase Inhibition and Analysis
- Immune Response and Inflammation
- 14-3-3 protein interactions
- Cancer-related gene regulation
- Natural product bioactivities and synthesis
- Immune cells in cancer
- Chemical Synthesis and Analysis
- Cancer-related Molecular Pathways
- Immune Cell Function and Interaction
- Kruppel-like factors research
- Inflammation biomarkers and pathways
- Herpesvirus Infections and Treatments
- Fungal Infections and Studies
- Bioactive Compounds and Antitumor Agents
- Microtubule and mitosis dynamics
- Autoimmune and Inflammatory Disorders Research
- Hormonal Regulation and Hypertension
- Polyamine Metabolism and Applications
- Caveolin-1 and cellular processes
Shenzhen University
2017-2024
Shenzhen University Health Science Center
2023-2024
Shenzhen Second People's Hospital
2023
Zhejiang University
2015-2020
The NLRP3 inflammasome is involved in a diverse range of inflammatory diseases. activation inflammasomes must be tightly regulated to prevent excessive inflammation, and the protein ubiquitination system reported one ways which regulated. However, deubiquitination regulatory mechanisms remain elusive. Here, we demonstrated that USP22 (ubiquitin specific peptidase 22) promotes degradation inhibits activation. deficiency or vivo silencing significantly increases alum-induced peritonitis...
TBK1 (TANK-binding kinase 1) is an essential receptor protein required for the innate immune response, but mechanisms underlying stability, especially those regulated via autophagy, remain poorly understood. Here, we demonstrate that USP19 (ubiquitin specific peptidase 19) interacts with and promotes lysosomal degradation chaperone-mediated autophagy (CMA). We observed had a canonical CMA motif, knocking down key proteins involved in (HSPA8/HSC70 or LAMP2A) inhibiting CMA-prevented...
Retinoic acid-inducible gene I (RIG-I) is a critical RNA virus sensor that initiates antiviral immune response through K63-linked ubiquitination. In this study, we demonstrated USP14, deubiquitinating enzyme, as negative regulator in responses by directly RIG-I. USP14 knockdown significantly enhanced RIG-I-triggered type IFN signaling and inhibited vesicular stomatitis (VSV) replication both mouse peritoneal macrophages THP1 cells. overexpression HeLa cells attenuated IFN-β expression...
Inflammatory bowel diseases (IBDs) are increasingly common characterized by chronic and relapsing inflammation of the gastrointestinal tract. NLRP3 might be a crucial regulator homeostatic balance intestine, but its upregulation leads to pyroptosis. Munronoid I is extracted purified from Munronia sinica , which has shown an anti-inflammatory effect, efficacy in IBD remains unproven. In this study, we attempted determine effect on regulate inflammasome activation pyroptosis IBD. Our data...
Succinate is at the crossroads of multiple metabolic pathways and plays a role in several immune responses acting as an inflammation signal. However, whether succinate regulates antiviral response remains unclear. Here, we found that production was reduced RAW264.7 cells during vesicular stomatitis virus (VSV) infection. Using diethyl to pretreat mouse peritoneal macrophages before VSV infection, interferon-β (IFN-β), chemokine (C–X–C motif) ligand 10 (CXCL-10), IFN-stimulated genes 15...
Abstract IκBα is a critical protein that inhibits NF-κB nuclear translocation and impairs NF-κB–mediated signaling. The abundance of determines the activation restoration inflammatory response. However, posttranslational regulation remains to be fully understood. In this study, we identified ubiquitin-specific protease 39 (USP39) as negative regulator in response by stabilizing basal IκBα. expression USP39 macrophages was reduced under LPS-induced inflammation. Knockdown or knockout...
The elevated Cyclin B1 expression contributes to various tumorigenesis and poor prognosis. could be regulated by ubiquitination deubiquitination. However, the mechanism of how is deubiquitinated its roles in human glioma remain unclear.Co-immunoprecipitation other assays were performed detect interacting USP39. A series vitro vivo experiments investigate effect USP39 on tumorigenicity tumor cells.USP39 interacts with stabilizes deubiquitinating B1. Notably, cleaves K29-linked polyubiquitin...
Inflammation is a biological process associated with multiple human disorders such as autoimmune diseases and metabolic diseases.Therefore, alleviation of inflammation important for disease prevention or treatment.Recently, deubiquitinating enzymes (DUBs), especially ubiquitin specific protease-7 (USP7) attracts increasing attention potential drug target inflammation.As an inhibitor USP7, P22077 has been used to study the roles USP7 in inflammatory response neuroblastoma growth.However, role...
Abstract R-spondins (RSPOs) are secreted signaling molecules that potentiate the Wnt/β-catenin pathway by cooperating with Wnt ligands. RSPO1 is crucial in tissue development and homeostasis. However, molecular mechanism which RSPOs activate remains elusive. In this study, we found could mediate degradation of Axin through ubiquitin–proteasome pathway. The results Co-IP showed recombinant protein promoted interaction between Axin1 CK1ε. Either knockout CK1ε gene or treatment CK1δ/CK1ε...
Glioma cells are characterized by high migration and invasion ability; however, the molecular mechanism behind both processes still remains to be investigated. Several studies have demonstrated that ubiquitin‐specific protease 39 (USP39) plays an oncogenic role in various cancer types. Here, we investigated expression function of USP39 patients with glioma. Oncomine database analysis revealed was significantly correlated poor overall survival Knockdown U251 U87 cell lines inhibited their...
Acute lung injury (ALI) is a severe disease with limited therapeutic strategies. Munronoid I, limonoid, which extracted and purified from Munronia sinica, exhibits effective anti-neoplastic activities. In this study, we attempted to determine the anti-inflammatory effects of I using both lipopolysaccharide (LPS)-induced in vivo murine ALI models vitro assays. Our results demonstrated that treatment ameliorated LPS-induced inflammation mice. Moreover, it also significantly inhibited...
Inflammation causes many diseases that are serious threats to human health. However, the molecular mechanisms underlying regulation of inflammation and inflammasome activation not fully understood which has delayed discovery new anti-inflammatory drugs urgent clinic need. Here, we found natural compound Teuvincenone F, was isolated purified from stems leaves Premna szemaoensis, could significantly inhibit lipopolysaccharide (LPS)-induced pro-inflammatory cytokines production NLRP3...
Fungal infections cause serious health problems, especially in patients with an immune-deficiency. Histone deacetylase 11 (HDAC11) mediates various immune functions, yet little is known about its role regulating host responses to fungal infection. Here we report that HDAC11 negatively controls antifungal immunity macrophages and dendritic cells. Deleting Hdac11 protects mice from morbidity markedly improves their survival rate upon systemic infection Candida albicans (C. albicans). Moreover,...
Autophagy is an important mechanism of cell homeostasis maintenance. As essential serine/threonine-protein kinases, casein kinase I family members affect tumorigenesis by regulating a variety cellular progression. However, the which they regulate autophagy remains unclear. We silenced CK1δ/ε in cancer cells and observed morphology, expression autophagy-related genes, its impact on growth viability. By inhibiting CK1δ/ε-induced upregulation we profiled regulatory growth. The inhibition tumor...
Casein kinase 1ε (CK1ε) and axis inhibitor 1 (AXIN1) are crucial components of the β-catenin destruction complex in canonical Wnt signaling. CK1ε has been shown to interact with AXIN1, but its physiological function role tumorigenesis remain unknown. In this study, we found that CK1δ/ε inhibitors significantly enhanced AXIN1 protein level colorectal cancer (CRC) cells through targeting CK1ε. Mechanistically, promoted degradation by ubiquitin-proteasome pathway promoting interaction E3...