A5101997656- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Pluripotent Stem Cells Research
- RNA modifications and cancer
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- Circular RNAs in diseases
- Tissue Engineering and Regenerative Medicine
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- Animal Genetics and Reproduction
- Endoplasmic Reticulum Stress and Disease
- Cancer Cells and Metastasis
- bioluminescence and chemiluminescence research
- CRISPR and Genetic Engineering
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Galectins and Cancer Biology
- Cell Adhesion Molecules Research
- interferon and immune responses
- Atherosclerosis and Cardiovascular Diseases
- Epigenetics and DNA Methylation
- Enzyme function and inhibition
- Lipoproteins and Cardiovascular Health
- Caveolin-1 and cellular processes
Henan Cancer Hospital
2015-2023
Zhengzhou University
2015-2023
University of Chinese Academy of Sciences
2020-2023
Shandong First Medical University
2023
Shandong Tumor Hospital
2023
Institute of Zoology
2023
Chinese Academy of Sciences
2023
Lishui City People's Hospital
2021
Affiliated Hospital of Jining Medical University
2017-2021
Xuzhou No.1 People's Hospital
2021
Abstract This study was aimed to explore the role of miR‐29b‐3p and PGRN in chondrocyte apoptosis initiation progress osteoarthritis ( OA ). Both were up‐regulated cartilage tissue from patients with . Transfection mimic into rat primary chondrocytes SW 1353 chondrosarcoma cells significantly suppressed expression release, induced apoptosis, inhibited proliferation scratch wound closure. By contrast, transfection inhibitor exhibited opposite effects. Moreover, secretion cartilaginous...
TBK1 (TANK-binding kinase 1) is an essential receptor protein required for the innate immune response, but mechanisms underlying stability, especially those regulated via autophagy, remain poorly understood. Here, we demonstrate that USP19 (ubiquitin specific peptidase 19) interacts with and promotes lysosomal degradation chaperone-mediated autophagy (CMA). We observed had a canonical CMA motif, knocking down key proteins involved in (HSPA8/HSC70 or LAMP2A) inhibiting CMA-prevented...
Breast cancer is one of the most common malignancies worldwide and second leading cause cancer-related mortality among females. miRNAs are a class small noncoding RNAs that aberrantly expressed in human cancers. Due to their size stability, have potential be efficacious clinical targets. MicroRNA-320a (miR-320a) has been shown dysregulated multiple malignancies. In present study, expression levels miR-320a were investigated 15 paraffin-embedded situ breast carcinoma 130 invasive tissues,...
BACKGROUND: Recent studies have demonstrated that MALAT1 is involved in cancer metastasis and recurrence it up-regulated cervical cancer, hepatocellular carcinoma, non-small cell lung (NSCLC) colorectal cancer. However, the role of esophageal squamous carcinoma (ESCC) still unclear. METHODS: The expression was evaluated tissue paired adjacent normal samples from 77 middle thoracic ESCC patients who received radical surgical resection using QRT-PCR. correlations between levels...
Hypoxia, a common phenomenon during the development of malignant solid tumors including breast cancer, serves to propagate cascade molecular pathways triggered by hypoxia-inducible factor-1α (HIF-1α). Carbonic anhydrase IX (CA IX), one target genes HIF-1α, has been reported be involved in progression tumors. The objective this study was investigate expression HIF-1α and CA hypoxia, involvement regulation migration invasion/metastasis its prognostic significance cancer. We used cobalt...
MicroRNA-27b (miR-27b) has been shown to play a role in the progression of many different forms cancer, but its specific relevance context non-small cell lung cancer (NSCLC) remains uncertain. As such, this study sought explore miR-27b NSCLC and mechanisms whereby it functions.We quantified target gene expression via quantitative real-time PCR (RT-qPCR).We then used functional including proliferation assays, migration assay, flow cytometry, western blotting functions vitro vivo. We...
Circular RNAs (circRNAs) have emerged as vital regulators in human cancers, including colorectal cancer (CRC). In this study, we aimed to explore the roles of circRUNX1 CRC.The levels circRUNX1, RUNX1 mRNA, solute carrier family 38 member 1 (SLC38A1) mRNA and microRNA-485-5p (miR-485-5p) were determined by quantitative real-time polymerase chain reaction (qRT-PCR) analysis. The protein level SLC38A1 was measured Western blot assay. Cell colony formation, migration, invasion apoptosis...
Sepsis-induced acute lung injury (ALI) culminates in multiple organ failure via uncontrolled inflammatory responses and requires effective treatment. Herein, we aimed to investigate the effect of calycosin (CA), a natural isoflavonoid, on sepsis-induced ALI. CA attenuated lipopolysaccharide (LPS) cecal ligation puncture (CLP)-induced structural damage cell infiltration tissues by histopathological analysis. significantly reduced wet/dry ratio, bronchoalveolar lavage fluid, myeloperoxidase...
We found that microRNA-320a (miR-320a) was an attractive prognostic biomarker in breast cancer (BC) previously, whereas its regulatory mechanism BC not well understood.Our aim to identify miR-320a target gene, examine the clinical relationship between and target, further explore functions of BC.In this study, downstream gene determined HEK-293T cells by dual luciferase reporter assay.Then western blotting immunohistochemistry were used assess expression fresh frozen (n=19, matched...
Long non-coding RNAs (lncRNAs) have been defined as vital regulators in the progression of human cancers, including colorectal cancer (CRC). intergenic non-protein coding RNA 667 (LINC00667) is a tumor promoter several types, while its role CRC remains to be unmasked. This study focused on exploring potential function and regulatory mechanism LINC00667 CRC.qRT-PCR analysis was applied detect expression cells. Loss-of assays revealed silencing regulating cell proliferation, apoptosis...
The fatality rate for cardiovascular disease (CVD) has increased in recent years and higher levels of triglyceride have been shown to be an independent risk factor atherosclerotic CVD. Dysfunction endothelial cells (ECs) is also a key APOC3 important molecule lipid metabolism that closely associated with hyperlipidemia developing But the direct effects on ECs were still unknown. This study was aimed at determining inflammation, chemotaxis exudation ECs. ELISA, qRT-PCR, immunofluorescence,...
BACKGROUND:LAMP2 is one of the major protein components lysosome. In addition to expression on lysosomal membrane, LAMP2 has also been found relocalizing cell surface some highly metastatic tumor cells. OBJECTIVE: The aim this study was detect levels LAM P2 and discuss its roles in esophageal squamous carcinoma (ESCC). METHODS: Six hundred ten tissue samples ESCC were collected construct microarrays, which stained by immunohistochemistry. RESULTS: After immunohistochemical staining, 596...
Porphyromonas endodontalis lipopolysaccharide (LPS) is considered to be correlated with the progression of bone resorption in periodontal and periapical diseases. Wnt5a has recently been implicated inflammatory processes, but its role unclear as a P. LPS-induced mediator osteoblasts. Tribbles homolog 3 (TRIB3) encodes pseudokinase linked inflammation certain situations. Here, we found that LPS induced expression dose- time-dependent manner it also upregulated translocation, phosphorylation...
Colon cancer (CC) belongs to a common of digestive system. Long non-coding RNAs (lncRNAs) are dysregulated in numerous cancers and affect their development. The function lncRNA CERS6 antisense RNA 1 (CERS6-AS1) CC remains unclear.CERS6-AS1 expression colon adenocarcinoma tissues cell lines was assessed by Cancer Genome Atlas database quantitative real-time polymerase chain reaction analysis. CERS6-AS1 analysed 5-ethynyl-2'-deoxyuridine, colony formation, flow cytometry, terminal...
Ubiquitin C-terminal Hydrolase-L5 (UCH-L5/UCH37), a member of the deubiquitinases (DUBs), suppresses protein degeneration via removing ubiquitin from distal subunit polyubiquitin chain. The activity UCH-L5 is enhanced when combines with proteasome 19S regulatory by Rpn13/Admr1 receptor and inhibited interacts NFRKB. But role in gliomas remains unknown. In this study, analysis 19 frozen 51 paraffin-embedded clinic pathological cases showed that expression glioma tissues was lower than normal...
Background . Syndecan-1 (SDC1/CD138) is a key cell surface adhesion molecule essential for maintaining morphology and the interactions with surrounding microenvironment. SDC1 tumor immunoexpression may be increased or decreased in epithelial malignant neoplasms compared to that adjacent non-neoplastic tissue, depending on type of carcinoma, it has been correlated various clinicopathological parameters patient prognosis. expression colorectal cancer (CRC) but relationship between prognosis...
Aim: Long noncoding RNA (lncRNA) B3GALT5-AS1 has been reported as a biomarker for cancer monitoring. This research aims to identify serum long new the diagnosis of colorectal (CRC) and evaluate its clinical value. Materials & methods: Serum expression levels were measured by quantitative real-time PCR. Results: The level in CRC patients was significantly lower than that healthy (p < 0.0001). Further exploration validated high related tumor node metastasis (TNM) stage = 0.008) histological...
Abstract ‘Human neural stem cells’ jointly drafted and agreed upon by experts from the Chinese Society for Stem Cell Research, is first guideline human cells (hNSCs) in China. This standard specifies technical requirements, test methods, regulations, instructions use, labelling packaging storage transportation requirements waste disposal hNSCs, which applicable to quality control hNSCs. It was originally released China Biology on 30 August 2022. We hope that publication of will facilitate...
Esophageal cancer is a common human malignant tumor with high mortality. Glandular epithelial markers, such as CAM5.2, can be expressed in esophageal squamous cell carcinoma (ESCC), but the clinical significance of these cells ESCC remains elusive.Immunohistochemical analysis CAM5.2 was performed on 604 specimens using tissue microarray. Our study design and population used retrospective cohorts based hospital information system pathological management which included medical information,...