A5022856643- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Pharmacological Receptor Mechanisms and Effects
- Neuroscience and Neuropharmacology Research
- Pain Mechanisms and Treatments
- Lipid Membrane Structure and Behavior
- Neurotransmitter Receptor Influence on Behavior
- Ion channel regulation and function
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- Bacterial Genetics and Biotechnology
- Photoreceptor and optogenetics research
- Nicotinic Acetylcholine Receptors Study
- Pharmacological Effects and Assays
- Protein Structure and Dynamics
- Stress Responses and Cortisol
- Insect Resistance and Genetics
- Advanced biosensing and bioanalysis techniques
- Regulation of Appetite and Obesity
- Cancer, Stress, Anesthesia, and Immune Response
- RNA Interference and Gene Delivery
- Neuroendocrine regulation and behavior
- Neurobiology and Insect Physiology Research
- Mycotoxins in Agriculture and Food
- Transgenic Plants and Applications
Institut des Neurosciences Cellulaires et Intégratives
2014-2023
Centre National de la Recherche Scientifique
2008-2023
Université de Strasbourg
2006-2023
Inserm
2005-2015
Institut de génétique et de biologie moléculaire et cellulaire
2003-2015
University of Calgary
2007
University of Regina
2007
École Supérieure de Biotechnologie de Strasbourg
1998-2003
École Normale Supérieure - PSL
1999-2002
University of Glasgow
2001
Opioid receptors are G protein-coupled (GPCRs) that modulate brain function at all levels of neural integration, including autonomic, sensory, emotional and cognitive processing. Mu (MOR) delta (DOR) opioid functionally interact in vivo, but whether interactions occur circuitry, cellular or molecular remains unsolved. To challenge the hypothesis MOR/DOR heteromerization brain, we generated redMOR/greenDOR double knock-in mice report dual receptor mapping throughout nervous system. Data...
GPCRs regulate a remarkable diversity of biological functions, and are thus often targeted for drug therapies. Stimulation GPCR by an extracellular ligand triggers receptor signaling via G proteins, this process is highly regulated. Receptor activation typically accompanied desensitization signaling, complex feedback regulatory which internalization postulated as key event. The in vivo significance poorly understood. In fact, the majority studies have been performed transfected cell systems,...
In addition to treating depression, antidepressant drugs are also a first-line treatment for neuropathic pain, which is pain secondary lesion or pathology of the nervous system. Despite widespread use these drugs, mechanism underlying their therapeutic action in this context remains partly elusive. The present study combined data collected male and female mice from model clinical setting understand how act. We show two distinct mechanisms by selective inhibitor serotonin noradrenaline...
Adolescence is a vulnerable period characterized by major cognitive changes. The mechanisms underlying the emergence of new functions are poorly understood. We find that long-term depression inhibitory transmission (iLTD) from parvalbumin-expressing (PV+) interneurons in hippocampal area Cornu Ammonis 2 (CA2) absent young mice but emerges at end adolescence. demonstrate maturation both perineuronal net (PNN) and signaling through ErbB4 required for this plasticity. Furthermore, we social...
Background: Microglia activation contributes to chronic pain and the adverse effects of opiate use such as analgesic tolerance opioid-induced hyperalgesia. Both mu opioid receptor (MOR) encoded by Oprm1/OPRM1 gene toll like 4 (TLR4) have been reported mediate these morphine a current question is whether microglia express Oprm1 transcript MOR protein. The aim this study was characterize Oprm1-MOR expression in naive murine human microglia, combining transcriptomics datasets previously...
In order to gain some insight into the mechanism of insertion membranes pore‐forming domain colicin A and structure its membrane‐bound form, circular dichroism (in near far ultraviolet), fluorescence ultraviolet spectroscopy experiments were carried out. Because water‐soluble form this fragment has been determined by X‐ray crystallography, these spectroscopic methods provided valuable information on secondary environment aromatic residues within two forms peptide. These results strongly...
G-protein-coupled receptors (GPCRs) mediate numerous physiological functions and represent prime therapeutic targets. Receptor trafficking upon agonist stimulation is critical for GPCR function, but examining this process in vivo remains a true challenge. Using knock-in mice expressing functional fluorescent delta opioid under the control of endogenous promoter, we visualized internalization native stimulation. We developed paradigm which animals were made dependent on morphine drug-paired...
The δ-opioid receptor (DOP) is an attractive pharmacological target due to its potent analgesic, anxiolytic and anti-depressant activity in chronic pain models. However, some but not all selective DOP agonists also produce severe adverse effects such as seizures. Thus, the development of novel requires a profound understanding their on phosphorylation, post-activation signaling dephosphorylation. Here we show that agonist-induced phosphorylation at threonine 361 (T361) serine 363 (S363)...
Phosphorylation of G protein-coupled receptors is considered an important step during their desensitization. In SK-N-BE cells, recently presented as a pertinent model for the studies human delta-opioid receptor, pretreatment with opioid agonist etorphine increased time-dependently rate phosphorylation 51-kDa membrane protein. Immunological characterization this protein antibody, raised against amino-terminal region cloned revealed that it corresponded to receptor. During prolonged treatment...
Light opens: Photocontrolled transmembrane passage of soluble dyes and delivery small peptides into mammalian cells has been achieved using azobenzene-modified polymers (AMPs) as permeabilizing agents. Irradiation with UV visible light triggers polarity switches upon cis–trans isomerization the azobenzene moieties. Photoresponsive permeability pore opening promoted by trans-AMPs, but not cis-AMPs, in supported lipid bilayers are observed. Detailed facts importance to specialist readers...
Functional interactions between the mu opioid receptor (MOR) and delta (DOR) represent a potential target for novel analgesics may drive effects of clinically approved drug eluxadoline treatment diarrhea-predominant irritable bowel syndrome. Although enteric nervous system (ENS) is likely site action, coexpression interaction MOR DOR in ENS are largely undefined. In present study, we have characterized distribution mouse examined MOR-DOR by using pharmacologic cell biology techniques....
Opioids are the gold standard for treatment of acute pain despite serious side effects in central and enteric nervous system. µ-opioid receptors (MOPs) expressed functional at terminals sensory axons, when activated by exogenous or endogenous ligands. However, presence function MOP along nociceptive axons remains controversial particularly naïve animals. Here, we characterized axonal MOPs immunofluorescence, ultrastructural, analyses. Furthermore, evaluated hypertonic saline as a possible...
Chronic exposure to the mycotoxin deoxynivalenol (DON) from grain-based food and feed affects human animal health. Known consequences include entereopathogenic immunotoxic defects; however, neurotoxic potential of DON has only come into focus more recently due observation behavioural disorders in exposed farm animals. can cross blood-brain barrier interfere with homeostasis/functioning nervous system, but underlying mechanisms action remain elusive. Here, we have investigated impact on mouse...