A5087543776- Liver Disease Diagnosis and Treatment
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Adipose Tissue and Metabolism
- Diabetes Treatment and Management
- Adipokines, Inflammation, and Metabolic Diseases
- Diet, Metabolism, and Disease
- Peroxisome Proliferator-Activated Receptors
- Silymarin and Mushroom Poisoning
- Menopause: Health Impacts and Treatments
- Pharmacology and Obesity Treatment
- Cardiovascular Disease and Adiposity
- Advanced Glycation End Products research
- Natural Antidiabetic Agents Studies
- Lipid metabolism and biosynthesis
- Eicosanoids and Hypertension Pharmacology
- Biochemical effects in animals
- Botanical Research and Applications
- Nutrition, Genetics, and Disease
- Lipid metabolism and disorders
- Cancer, Lipids, and Metabolism
- Fatty Acid Research and Health
- Advanced Nanomaterials in Catalysis
- Biochemical Analysis and Sensing Techniques
- Meat and Animal Product Quality
Institute of Clinical and Experimental Medicine
2019-2025
Charles University
2021-2024
Recent studies suggest that treatment with SGLT-2 inhibitors can reduce hepatic lipid storage and ameliorate non-alcoholic fatty liver disease (NAFLD) development beyond their glycemic benefits. However, the exact mechanism involved is still unclear. We investigated metabolic effect of empagliflozin (10 mg/kg/day for eight weeks) on NAFLD its complications using HHTg rats as a non-obese prediabetic rat model. Empagliflozin reduced neutral triacylglycerols lipotoxic diacylglycerols in was...
Background: Hypertriglyceridemia has serious health risks such as cardiovascular disease, type 2 diabetes mellitus, nephropathy, and others. Fenofibrate is an effective hypolipidemic drug, but its benefits for ameliorating disorders associated with hypertriglyceridemia failed to be proven in clinical trials. Methods: To search possible causes of this situation possibilities their favorable influence, we tested the effect FF monotherapy combination fenofibrate silymarin on metabolic a unique...
Monounsaturated fatty acids (MUFA) are understood to have therapeutic and preventive effects on chronic complications associated with type 2 diabetes mellitus (T2DM); however, there differences between individual MUFAs. Although the of palmitoleic acid (POA) still debated, POA can regulate glucose homeostasis, lipid metabolism, cytokine production, thus improving metabolic disorders. In this study, we investigated compared oleic (OA) supplementation insulin sensitivity, inflammation in a...
Magnetic nanoparticles (MNPs) modified with tannic acid (TA) have shown remarkable success as an antioxidant and antimicrobial therapeutic agent. Herein, we report a synthetic procedure for the preparation of silica-coated MNPs N-acetylcysteine-modified chitosan TA. This was achieved by free-radical grafting NAC onto (CS), layer-by-layer technique modifying negatively charged MNP@SiO2 positively CS-NAC, crosslinking CS The metabolic effects MNP@SiO2-CS-NAC MNP@SiO2-CS-NAC-TA were tested in...
Background and aims Recent studies suggest that empagliflozin reduces total cardiovascular mortality in both diabetic nondiabetic subjects. Although the exact mechanism is unclear, it understood to positively affect myocardial energetics, including metabolism of ketone bodies, lipids, fatty acids. In this study, we compared effects on lipid heart liver a prediabetic rat model with severe dyslipidemia. Materials methods Wistar rats served as control group, while hereditary...
Magnetic γ-Fe2O3/CeOx nanoparticles were obtained by basic coprecipitation/oxidation of iron chlorides with hydrogen peroxide, followed precipitation Ce(NO3)3 ammonia. The appearance CeOx on the magnetic particle surface was confirmed X-ray photoelectron spectroscopy (XPS), powder diffraction (XRD), and elemental analysis; a magnetometer used to measure properties γ-Fe2O3/CeOx. relatively high saturation magnetization particles (41.1 A∙m2/kg) enabled separation. functionalized...
Background and aims: Low-grade chronic inflammation plays an important role in the pathogenesis of metabolic syndrome, type 2 diabetes their complications. In this study, we investigated effects salsalate, a non-steroidal anti-inflammatory drug, on disturbances animal model prediabetes-a strain non-obese hereditary hypertriglyceridemic (HHTg) rats. Materials Methods: Adult male HHTg Wistar control rats were fed standard diet without or with salsalate delivering daily dose 200 mg/kg body...
Gliflozins (inhibitors of sodium-glucose cotransporter 2) show many beneficial actions beyond their antidiabetic effects. The underlying mechanisms these additional protective effects are still not well understood, especially under non-diabetic conditions. Therefore, we analyzed the empagliflozin in young (3-month-old) and adult (12-month-old) male spontaneously hypertensive rats (SHR) expressing human C-reactive protein (CRP) liver. SHR-CRP a model metabolic syndrome, inflammation, organ...
A combination of liver and heart dysfunction worsens the prognosis human survival. The aim this study was to investigate whether empagliflozin (a sodium-glucose transporter-2 inhibitor) has beneficial effects not only on cardiac renal function but also hepatic function. Adult (6-month-old) male spontaneously hypertensive rats (SHR) were fed a high-fat diet (60% fat) for four months induce steatosis mild failure. For last two months, treated with (empa, 10 mg.kg
Introduction . The development of metabolic syndrome-associated renal dysfunction is exacerbated by a number factors including dyslipidemia, ectopic deposition lipids and their toxic metabolites, impairment lipid metabolism, insulin resistance. Renal also affected the production proinflammatory profibrotic secreted from adipose tissue, which can in turn directly impair kidney cells potentiate In this study, we investigated manifestation accumulation its effect on model syndrome—the...
Ovarian hormone deficiency leads to increased body weight, visceral adiposity, fatty liver and disorders associated with menopausal metabolic syndrome. To better understand the underlying mechanisms of these in their early phases development, we investigated effect ovariectomy on lipid glucose metabolism. Compared sham-operated controls, ovariectomized Wistar female rats markedly whole adipose tissue weight (p ˂ 0.05) exhibited insulin resistance peripheral tissues. Severe hepatic...
Metformin can reduce cardiovascular risk independent of glycemic control. The mechanisms behind its non-glycemic benefits, which include decreased energy intake, lower blood pressure and improved lipid fatty acid metabolism, are not fully understood. In our study, metformin treatment reduced myocardial accumulation neutral lipids—triglycerides, cholesteryl esters the lipotoxic intermediates—diacylglycerols lysophosphatidylcholines in a prediabetic rat model (p < 0.001). We observed an...
The new antidiabetic drugs, gliflozins, inhibit sodium-glucose transporter-2 in renal proximal tubules promoting glucose and sodium excretion. This leads not only to a significant improvement of control but also the reduction blood pressure body weight both diabetic patients experimental models. We examined whether these beneficial effects can be achieved non-diabetic hypertensive model, namely Ren-2 transgenic rats (TGR). Adult 6-month-old TGR their normotensive controls (Hannover...
The combination of plant-derived compounds with anti-diabetic agents to manage hepatic steatosis closely associated diabetes mellitus may be a new therapeutic approach. Silymarin, complex bioactive substances extracted from Silybum marianum, evinces an antioxidative, anti-inflammatory, and hepatoprotective activity. In this study, we investigated whether metformin (300 mg/kg/day for four weeks) supplemented micronized silymarin (600 mg/kg/day) would effective in mitigating fatty liver...
: Metabolic-dysfunction-associated steatotic liver disease (MASLD) represents a major clinical complication of obesity.
Recently, red beetroot has attracted attention as a health-promoting functional food. Studies have shown that administration can reduce blood pressure and ameliorate parameters of glucose lipid metabolism; however, mechanisms underlying these beneficial effects are not yet fully understood. In the current study, we analysed on metabolism in two models metabolic syndrome: (i) transgenic spontaneously hypertensive rats expressing human C-reactive protein (SHR-CRP rats), (ii) hereditary...
Excessive methylglyoxal (MG) production contributes to metabolic and vascular changes by increasing inflammatory processes, disturbing regulatory mechanisms exacerbating tissue dysfunction. MG accumulation in adipocytes leads structural functional changes. We used transcriptome analysis investigate the effect of on visceral adipose hereditary hypetriglyceridaemic rats, a non-obese model syndrome. Compared controls, 4-week intragastric administration impaired glucose tolerance (p < 0.05)...
Metabolic syndrome (MetS) is an important cause of worldwide morbidity and mortality. Its complex pathogenesis includes, on the one hand, sedentary lifestyle high caloric intake, and, other there a clear genetic predisposition. PD (Polydactylous rat) animal model hypertriglyceridemia, insulin resistance, obesity. To unravel pathophysiologic background this phenotype, we compared morphometric metabolic parameters as well liver transcriptomes among PD, spontaneously hypertensive rat, Brown...
Hypolipidemic and cardioprotective effects of statins can be associated with the development myopathies new-onset type 2 diabetes. These adverse may related to increased oxidative stress. The plant extract silymarin (SM) is known for its antioxidant anti-inflammatory actions. We tested hypothesis that combination atorvastatin (ATV) SM could improve therapy efficacy eliminate some negative statin on hypertriglyceridemia-induced metabolic disorders. Hereditary hypertriglyceridemic rats were...
The mechanisms behind the cardiovascular and renal benefits of empagliflozin is not fully understood. positive impact medication on mortality can be solely attributed to its antidiabetic effect, with a metabolic mechanism possibly involved. To investigate effects treatment (10 mg/kg/day for 6 weeks), we used an adult male rat model serious vascular complications associated syndrome prediabetes. Impaired glucose tolerance, severe albuminuria impaired insulin sensitivity were induced by...
If menopause is really independent risk factor for cardiovascular disease still under debate. We studied if ovariectomy in the model of insulin resistance causes changes, to what extent are these changes reversible by estradiol substitution and they accompanied other organs tissues. Hereditary hypertriglyceridemic female rats were divided into three groups: ovariectomized at 8th week (n = 6), with 17-β sham group 5). The strain abdominal aorta measured ultrasound, expression vascular genes,...
We investigated the sex-dependent effects of inflammatory responses in visceral adipose tissue (VAT) and perivascular (PVAT), as well hematological status, relation to cardiovascular disorders associated with prediabetes. Using male female hereditary hypertriglyceridemic (HHTg) rats-a nonobese prediabetic model featuring dyslipidemia, hepatic steatosis, insulin resistance-we found that HHTg females exhibited more pronounced hypertriglyceridemia than males, while males had higher non-fasting...
Menopause brings about profound physiological changes, including the acceleration of insulin resistance and other abnormalities, in which adipose tissue can play a significant role. This study analyzed effect ovariectomy estradiol substitution on metabolic parameters transcriptomic profile prediabetic females hereditary hypertriglyceridemic rats (HHTgs). The HHTgs underwent (OVX) or sham surgery (SHAM), half OVX group received 17β-estradiol (OVX+E2) post-surgery. Ovariectomy resulted weight...
Recently, we have identified a recessive mutation, an abnormal coat appearance in the BXH6 strain, member of HXB/BXH set recombinant inbred (RI) strains. The RI strains were derived from spontaneously hypertensive rat (SHR) and Brown Norway (BN-Lx) progenitors. Whole genome sequencing mutant rats 195875980 G/A mutation tuftelin 1 (Tuft1) gene on chromosome 2, which resulted premature stop codon. Compared with wild-type rats, BXH6-Tuft1 exhibited lower body weight due to reduced visceral fat...