A5031233719- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Alzheimer's disease research and treatments
- Immunotherapy and Immune Responses
- Microtubule and mitosis dynamics
- Oral and gingival health research
- Oral microbiology and periodontitis research
- Cytokine Signaling Pathways and Interactions
- Parkinson's Disease Mechanisms and Treatments
- Reproductive System and Pregnancy
- Immune Response and Inflammation
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- Dermatology and Skin Diseases
- Autophagy in Disease and Therapy
- Computational Drug Discovery Methods
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Parasites and Host Interactions
- NF-κB Signaling Pathways
- Cancer-related Molecular Pathways
- Synthesis and biological activity
- Respiratory Support and Mechanisms
- Prion Diseases and Protein Misfolding
- Mesenchymal stem cell research
- Bioactive Compounds and Antitumor Agents
Sun Yat-sen University
2017-2022
Third Affiliated Hospital of Sun Yat-sen University
2021
New York State Office for People With Developmental Disabilities
1997-1998
Alzheimer disease is characterized by a specific type of neuronal degeneration in which the microtubule associated protein τ abnormally hyperphosphorylated causing disruption network. We have found that phosphorylation human (τ3L) A‐kinase, GSK‐3 or CK‐1 inhibits its assembly‐promoting and microtubule‐binding activities. However, inhibition these activities significantly increased if prephosphorylated A‐kinase CK‐1. The most potent observed combination with GSK‐3. Under conditions, only very...
Abstract Background The R1441G mutation in the leucine-rich repeat kinase 2 (LRRK2) gene results late-onset Parkinson’s disease (PD). Peripheral inflammation and gut microbiota are closely associated with pathogenesis of PD. Chronic periodontitis is a common type peripheral inflammation, which Porphyromonas gingivalis (Pg), most bacterium causing chronic periodontitis, can cause alteration microbiota. It not known whether Pg-induced dysbiosis plays role pathophysiology Methods In this study,...
Recent studies indicated that CXCR5+CD8+ T cells in lymph nodes could eradicate virus-infected target cells. However, the current study we found a subset of germinal centers from human tonsils or are predominately memory express CD45RO and CD27. The involvement humoral immune responses is suggested by their localization B cell follicles concomitant expression costimulatory molecules, including CD40L ICOS after activation. In addition, produced significantly higher levels IL-21, IFN-γ IL-4 at...
Inflammatory bowel diseases (IBD) are prevalent and debilitating diseases; their clinical remedy is desperately unmet. Mesenchymal stem cells (MSCs) pluripotent with multiple immunomodulatory effects, which attributed to efficacy in the IBD rodent model. Optimization of MSC regimes a crucial step for further application. Wogonin flavonoid-like compound, showed extensive adjuvant effects. This research aimed at investigating whether how boosted therapeutic efficiency MSCs on DSS-induced...
Several kinases have been shown to phosphorylate tau protein at Ser‐262, an important site involved in the regulation of binding microtubules. In this study we compared phosphorylation Ser‐262 by CaMKII, PhK and PKA vitro as determined radioimmunoblots developed monoclonal antibody 12E8 which recognizes P‐Ser‐262 P‐Ser‐356; Ab‐262, a polyclonal is specific unphosphorylated tau. We found that was several times more effective CaMKII than or PhK. Employing rat brain extract source all KN‐62,...
Background and Purpose: Both NLRP3 inflammasome chemokines are involved in the initiation development of acute lung inflammation, but underlying mechanism is still elusive. The present study investigated role recruiting neutrophils early phase injury. Methods: In an endotoxin (lipopolysaccharide [LPS])-induced injury model, we measured severity, myeloperoxidase (MPO) activity chemokine profiles wild-type (WT) knockout (NLRP3 –/– ) mice, then identified key by specific antibody blockage....
γδT cells are non-conventional T and serve as the bridge for connecting innate adaptive immune systems. form a substantial population at barrier sites play an important role in development of physiology, inflammation, autoimmune diseases tumors. not only distribute maternal-fetal interface during pregnancy but also non-pregnant uterus. However, phenotypes functions uterus were clear. In current study, we found that percentages significantly higher than peripheral blood most distributed...
Tissue-resident memory γδT cells at mucosal and epithelial sites play an important role for pathogen clearance, immunosurveillance, participating in physiological processes. Different from other barrier sites, the immune uterus face protection against infections tolerate allogeneic fetus during a successful pregnancy. In previous study, we found that tissue-resident were enriched both human murine highly expressed IL-17 promoted invasion of trophocytes vitro. current but not blood or spleens...
Wogonin (5,7-dihydroxy-8-methoxyflavone) is an ingredient of the extracts from Scutellaria baicalensis, which has documented a wide spectrum anti-inflammatory and antitumor activities, including inhibiting regulatory T cells, regulating effector cell functions, mediating macrophage immunity. However, potential effect on B cells not been fully understood. Here, our results showed that inhibited IL-10 secretion in cells. When purified were activated by lipopolysaccharide (LPS) vitro, amount...
B cells play a key role in humoral immune responses by producing antibodies. Although there are numerous research on memory definition markers and cytokines cell development, different studies have yielded contradictory conclusions due to species studied, the stimulating agents used. In current study, we conducted detailed characterization of human CBMCs, PBMCs tonsil, including expression Igs, activation markers. Furthermore, found that considerable amounts IgA IgG were expressed CD27
Many kinds of lymphocytes are involved in Schistosoma japonicum (S. japonicum) infection-induced disease. γδ T cells comprise a small number innate that quickly respond to foreign materials. In this study, the role lung S. japonicum-infected C56BL/6 mice was investigated. The results demonstrated infection induces cell accumulation lung, expressing higher levels CD25, MHCII, CD80, and PDL1, lower CD127 CD62L (P < 0.05). intracellular cytokines staining illustrated percentages IL-4-, IL-10-,...
Abstract The field of tissue‐resident B cells has received increasing attention, yet the feature tissue in respiratory system is unclear. Here, we first show that non‐circulating obtained from nasal, trachea and lung tissues are numerically phenotypically distinct their circulating counterparts. Analysis single cell transcriptome sequence identified multiple differentially expressed genes between cells, which illustrated heterogeneity. Furthermore, found high expression CXCR3 on chemokine...
Tissue-resident memory T cells (TRM) are different from effector (TEM) and central (TCM) contribute to the protective immunity against local challenges. Currently, we found that CD4+ CD8+ TRM in nasal mucosa, trachea, lungs, lavage fluids were heterogeneous on expression of CD69 CD103 as well production cytokines including IFN-γ, IL-2, TNF-α. After intranasal vaccination mice with BCG, respiratory tissues expressed higher levels chemokine CXCL16 CXCR6 CXCL16. In addition, antigen-specific...
<p indent="0mm">Most effector T cells are known to die as only a few of them became memory following immune responses. According their distribution, function, and transcription factors, can be divided into cells, central tissue resident (T<sub>RM</sub>). The do not circulate in the blood lymph nodes but found non-lymphoid tissues, which includes skin mucous membranes, urogenital system, brain, respiratory lung, liver tissues. form play defense response directly quickly when pathogens invade...
Objective To investigate the inhibitory effects of Tofacitinib on 4-hydroxy-3-methyl-but-enyl pyrophosphate(HMBPP)-specific γδT cells. Method We used HMBPP as an antigen to stimulate cells, in order study tofacitinib production cytokines, activation, proliferation and phosphorylation STATs HMBPP-specific cells. Results Stimulation with could induce interferon(IFN)-γ tumor necrosis factor(TNF)-α cells.The IFN-γ showed a dose-dependent trend, while TNF-α reached peak at 12 hours...
Abstract Background The R1441G mutation in the leucine-rich repeat kinase 2 (LRRK2) gene results late-onset Parkinson’s disease (PD). Peripheral inflammation and gut microbiota are closely associated with pathogenesis of PD. Chronic periodontitis is a common type peripheral inflammation, which Porphyromonas gingivalis (Pg), most bacterium causing chronic periodontitis, can cause alteration microbiota. It not known whether Pg-induced dysbiosis plays role pathophysiology Methods In this study,...