A5013057115- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Cell Image Analysis Techniques
- Cellular Mechanics and Interactions
- Amyotrophic Lateral Sclerosis Research
- Retinal Development and Disorders
- Image Processing Techniques and Applications
- Cell Adhesion Molecules Research
- Genetics and Neurodevelopmental Disorders
- Genetic Neurodegenerative Diseases
- Advanced Fluorescence Microscopy Techniques
- Cellular transport and secretion
- Nicotinic Acetylcholine Receptors Study
- Neurobiology and Insect Physiology Research
- Glaucoma and retinal disorders
- Ion Channels and Receptors
- Neurogenetic and Muscular Disorders Research
- Pain Mechanisms and Treatments
- Microtubule and mitosis dynamics
- Cardiac electrophysiology and arrhythmias
- Parkinson's Disease Mechanisms and Treatments
- Retinal Diseases and Treatments
- Topological and Geometric Data Analysis
- Lipid Membrane Structure and Behavior
- Neurogenesis and neuroplasticity mechanisms
National Institute of Genomic Medicine
2025
Orion Corporation (Finland)
2022-2023
Instituto Cajal
1994-2021
University of the Basque Country
2017-2021
Instituto Biofisika
2021
Lockheed Martin (United States)
2021
Consejo Superior de Investigaciones Científicas
1994-2019
Vall d'Hebron Institut de Recerca
2019
Centre for Biomedical Network Research on Rare Diseases
2019
Universitat Autònoma de Barcelona
1992-2017
The possibility of changing the number synapses may be an important asset in treatment neurological diseases. In this context, synaptogenic role phosphoinositide-3-kinase (PI3K) signaling cascade has been previously demonstrated Drosophila. This study shows that with a PI3K-activating transduction peptide is able to promote synaptogenesis and spinogenesis primary cultures rat hippocampal neurons, as well CA1 neurons vivo. culture, increases synapse density independently cell density, culture...
Learning and memory deficits are early clinical manifestations of Huntington's disease (HD). These cognitive impairments have been mainly associated with frontostriatal HD pathology; however, compelling evidence provided by several murine models suggests that the hippocampus may contribute to synaptic dysfunction in HD. The neurotrophin receptor p75(NTR) negatively regulates spine density, which is learning memory; therefore, we explored whether disturbed function could Here, determined...
Down syndrome (DS) is the most frequent genetic cause of mental retardation. Cognitive dysfunction in these patients correlated with reduced dendritic branching and complexity, along fewer spines abnormal shape that characterize cortical neuronal profile DS. DS phenotypes are caused by disruptive effect specific trisomic genes. Here, we report overexpression dual-specificity tyrosine phosphorylation-regulated kinase 1A, DYRK1A, sufficient to produce alterations observed patients. Engineered...
Abstract Excitatory amino acids exert a depolarizing action on central nervous system cells through an increase in cationic conductances. Non‐NMDA receptors have been considered to be selectively permeable Na + and K , while Ca 2+ influx has thought occur the NMDA receptor subtype. Recently, however, expression of cloned non‐NMDA subunits shown that α ‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid (AMPA) are whenever lacks particular subunit (edited GluR‐B). The behaviour recombinant...
The objective of this study was to investigate the association functional variants human CX3CR1 gene (Fractalkine receptor) with risk Amyotrophic Lateral Sclerosis (ALS), survival and progression rate disease symptoms in a Spanish ALS cohort. 187 patients (142 sporadic [sALS] 45 familial) 378 controls were recruited. We investigated V249I (rs3732379) T280M (rs3732378) genotypes their haplotypes as predictors survival, (as measured by ALSFRS-R FVC decline) suffering disease. results indicated...
ABSTRACT Studies suggest that vegetarians and Asians have lower mortality rates from prostate cancer compared to men who follow a Western diet. β‐sitosterol, key compound of plant‐based diets, has been found induce significant changes in the ultrasonic structure prostatic adenomas, making it promising candidate for further research. Consequently, we investigated potential β‐sitosterol synthetic derivative 2 as potent inhibitors androgen synthesis, critical process growth survival tumor LNCaP...
Associative memory is the main type of learning by which complex organisms endowed with evolved nervous systems respond efficiently to certain environmental stimuli. It has been found in different multicellular species, from cephalopods humans, but never individual cells. Here we describe a motility pattern consistent associative conditioned behavior microorganism Amoeba proteus. We use controlled direct-current electric field as stimulus, and specific chemotactic peptide unconditioned...
Trabecular meshwork (TM) cell shape, volume, contractility and their interactions with extracellular matrix determine outflow facility. Because volume seems essential to TM function, this study was conducted investigate further the ionic channels receptors involved in regulatory decrease roles modulating facility.Primary cultures of bovine cells were used. K(+) Cl(-) currents studied whole-cell patch clamping. Swelling induced by hypotonic shock. [Ca(2+)](i) measured loaded fura-2. Bovine...
The establishment of neural circuits depends on the ability axonal growth cones to sense their surrounding environment en route target. To achieve this, a coordinated rearrangement cytoskeleton in response extracellular cues is essential. Although previous studies have identified different chemotropic and adhesion molecules that influence development, molecular mechanism by which these signals control remains poorly understood. Here, we show vivo conditional ablation focal kinase gene (Fak)...
The PI3K-dependent activation of AKT results in the inhibition GSK3β most signaling pathways. These kinases regulate multiple neuronal processes including control synapse number as shown for Drosophila and rodents. Alzheimer disease's patients exhibit high levels circulating and, consequently, pharmacological strategies based on antagonists have been designed. approach, however, has yielded inconclusive so far. Here, we carried out a comparative study rats addressing role synaptogenesis. In...
PI3K activation promotes the formation of synaptic contacts and dendritic spines, morphological features glutamatergic synapses that are commonly known to be related learning processes. In this report, we show in vivo administration a peptide activates signaling pathway increases spine density rat hippocampus enhances animals' cognitive abilities, while electrophysiological recordings results enhancement Schaffer stratum lacunosum moleculare inputs. Morphological characterization spines...
Cellular motility is the basis for cancer cell invasion and metastasis. In case of breast cancer, most common type among women, metastasis represents devastating stage disease. The central role cellular in development emphasizes importance understanding specific mechanisms involved this process. context, tumor would be consequence a loss or defect that control cytoskeletal remodeling. Profilin I belongs to family small actin binding proteins are thought assist filament elongation at leading...
Phosphatase and tensin homolog on chromosome 10 (PTEN) is a tumor suppressor autism-associated gene that exerts an important influence over neuronal structure function during development. In addition, it participates in synaptic plasticity processes adulthood. As attempt to assess developmental mechanisms by which PTEN can modulate cognitive function, we studied the consequences of 2 different genetic manipulations mice: presence additional genomic copies Pten (Ptentg) knock-in truncated...
Our objective was to investigate the prevalence of FUS/TLS mutations in a Catalan familial ALS cohort undergoing mutational study for SOD1 2006. We screened 25 probands from non-SOD1 families mutations. identified two FALS with One carried C-to-T transition at nucleotide position 1561 (c.1561C>T) producing p.R521C sequence change protein level. The phenotype characterized by young age onset (38.2 years old), proximal limb girdle weakness, predominant lower motor neuron signs and dropped...
Abstract The study of regulated vesicle exocytosis, which underlies neurotransmitter and neuropeptide release, has benefited from a convergence several independent approaches. These include the use genetically tractable organisms model preparations that allow direct characterization presynaptic ionic currents. Aiming for comprehensive analysis we had already developed Drosophila preparation in electrophysiological recordings peptidergic terminals are feasible. Here, report on Ca 2+ ‐channel...
In nonexcitable cells, G(q)-coupled membrane receptor activation induces a biphasic increase in intracellular calcium ([Ca(2+)](i)) expressed as an initial IP(3)-dependent release from stores followed by sustained Ca(2+) influx the extracellular space that involves store-operated channels (SOCs). trabecular meshwork (TM) contractile agonists such bradykinin (BK) and endothelin-1 (ET-1) induce this type of signaling. Given outflow is modified tissue contractility, authors characterized SOCs...
Profilin has been implicated in cell motility and a variety of cellular processes, such as membrane extension, endocytosis, formation focal complexes. In vivo, profilin replenish the pool ATP-actin monomers by increasing rate nucleotide exchange ADP-actin for ATP-actin, promoting incorporation new actin at barbed end filaments. For this report, we generated membrane-permeable version I (PTD4-PfnI) alteration intracellular levels taking advantage protein transduction technique. We show that...