A5101541189- biodegradable polymer synthesis and properties
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Microbial Fuel Cells and Bioremediation
- Bacteriophages and microbial interactions
- Biological and pharmacological studies of plants
- Plant and animal studies
- Glycosylation and Glycoproteins Research
- Amino Acid Enzymes and Metabolism
- CRISPR and Genetic Engineering
- Carbon dioxide utilization in catalysis
- Enzyme Catalysis and Immobilization
- Toxin Mechanisms and Immunotoxins
- Wine Industry and Tourism
- Seed Germination and Physiology
- Microplastics and Plastic Pollution
- Ginseng Biological Effects and Applications
- Plant tissue culture and regeneration
- Competency Development and Evaluation
- Advanced SAR Imaging Techniques
- Heavy metals in environment
- Cancer Mechanisms and Therapy
- Cancer Research and Treatments
- RNA regulation and disease
- Engineering Education and Curriculum Development
Zhejiang University of Technology
2024
National University of Defense Technology
2024
Tianjin Medical University
2023
Renmin Hospital of Wuhan University
2023
Wuhan University
2023
Chongqing Vocational Institute of Engineering
2023
Tianjin Agricultural University
2021-2022
Center for Life Sciences
2012-2019
Tsinghua University
2010-2019
Tianjin University of Traditional Chinese Medicine
2019
Bacteria, exemplified by enteropathogenic Escherichia coli (E. coli), rely on elaborate acid resistance systems to survive acidic environment (such as the stomach). Comprehensive understanding of bacterial is important for prevention and clinical treatment. In this study, we report a previously uncharacterized type system in E. that relies L-glutamine (Gln), one most abundant food-borne free amino acids. Upon uptake into coli, Gln converted L-glutamate (Glu) acid-activated glutaminase YbaS,...
5-Aminolevulinic acid (ALA), an important cell metabolic intermediate useful for cancer treatments or plant growth regulator, was produced by recombinant Escherichia coli expressing the codon optimized mitochondrial 5-aminolevulinic synthase (EC: 2.3.1.37, hem1) from Saccharomyces cerevisiae controlled via plasmid encoding T7 expression system with a RNA polymerase. When more efficient autoinduced approach free of IPTG applied, containing antibiotic-free stabilized able to produce 3.6 g/L...
Tropane alkaloids (TA) including hyoscyamine, anisodamine, scopolamine and anisodine, are used medicinally as anticholinergic agents with increasing market demand, so it is very important to improve TA production by metabolic engineering strategy. Here, we report the simultaneous introduction of genes encoding branch-controlling enzyme tropinone reductase I (TRI, EU424321) downstream rate-limiting hyoscyamine-6β-hydroxylase (H6H, EF187826) involved in biosynthesis into Anisodus acutangulus...
A couple of DNA ligation-independent cloning (LIC) methods have been reported to meet various requirements in metabolic engineering and synthetic biology. The principle LIC is the assembly multiple overlapping fragments by single-stranded (ss) overlaps annealing. Here we present a method generate based on Nicking Endonucleases (NEases) for LIC, was termed NE-LIC. Factors related efficiency were optimized this study. This NE-LIC allows generating 3'-end or 5'-end ss lengths assembly. We...
Ginsenosides have previously been demonstrated to effectively inhibit cancer cell growth and survival in both animal models lines. However, the specific ginsenoside component that is active ingredient for treatment through interaction with a target protein remains unknown. By an integrated quantitative proteomics approach via affinity mass spectrum (MS) technology, we deciphered core structure of derived from crude extracts ginsenosides progressed toward identifying mediates its anticancer...
Abstract Background Microbial polyhydroxyalkanoates (PHA) are biopolyesters consisting of diverse monomers. PHA synthase PhaC2 Ps cloned from Pseudomonas stutzeri 1317 is able to polymerize short-chain-length (scl) 3-hydroxybutyrate (3HB) monomers and medium-chain-length (mcl) 3-hydroxyalkanoates (3HA) with carbon chain lengths ranging C6 C12. However, the scl mcl production in Escherichia coli expressing limited very low yield. Results To improve a wide range monomer compositions E. ,...
Growing evidence has revealed high heterogeneity of fine root networks in both structure and function, with different orders corporately maintaining trees' physiological activities. However, little information is available on how trees responds to environmental stresses. We examined concentrations seven potentially toxic metals (Cr, Ni, Cu, Zn, As, Cd, Pb) within their correlations morphological macro-elemental traits six Chinese subtropical trees. The contributions roots fine-root metal...
Neovascular age-related macular degeneration AMD (nAMD) is characterized by choroidal neovascularization (CNV) and could lead to irreversible blindness. However, anti-vascular endothelial growth factor (VEGF) therapy has limited efficacy. Therefore, we generated a chimpanzee adenoviral vector (AdC68-PFC) containing three genes, pigment endothelial-derived (PEDF), soluble fms-like tyrosine kinase-1 (sFlt-1), forms of CD59 (sCD59), treat nAMD. The results showed that AdC68-PFC mediated strong...
Penicillium marneffei is a dimorphic, pathogenic fungus in Southeast Asia that mostly afflicts immunocompromised individuals. As the only dimorphic member of genus, it goes through phase transition from mold to yeast form, which believed be requisite for its pathogenicity. Mp1p, cell wall antigenic mannoprotein existing widely yeast, hyphae, and conidia fungus, plays vital role host immune response during infection. To understand function we have determined x-ray crystal structure ligand...
Based on randomized block design of experiment, agronomic traits and yields 14 F1 generations Dendrobium officinale were determined. The results showed that the differences in among families significant, hybrid vigor was obvious. Families 6b x 2a, 9 66 78 69 selected with remarkable superiority yields, product customization. Correlation analysis between plant height, stem diameter, leaf number, blade length width all significantly correlated biological economic yields. Among which, number...
Background Chronic pressure overload triggers pathological cardiac hypertrophy that eventually leads to heart failure. Effective biomarkers and therapeutic targets for failure remain be defined. The aim of this study is identify key genes associated with by combining bioinformatics analyses molecular biology experiments. Methods Comprehensive tools were used screen related overload-induced hypertrophy. We identified differentially expressed (DEGs) overlapping three Gene Expression Omnibus...
A long-standing objective of metabolic engineering has been to exogenously increase the expression target genes. In this research, we proposed permanent RNA replication system using DNA as a template store genetic information in bacteria. We selected Qβ phage prototype and made many improvements achieve gene enhancement directly by increasing mRNA abundance. First, identified endogenous Rnc, knockout which significantly improved efficiency. Second, elucidated essential elements for optimized...
With the rapid development of synthetic biology, demand for assembling multiple DNA (genes) fragments into a large circular structure in one step has dramatically increased. However, constructions most DNA, there are two contradictions ligation/assembly and transformation steps. The consists different reactions: 1) between any pieces linear form DNA; 2) cyclization (or self-ligation) single DNA. first contradiction is that bimolecular requires higher concentration while favors lower one;...
Using phenol-sulfuric acid method and hot-dip of alcohol-soluble extracts, the contents polysaccharides extracts in 11 F1 generations Dendrobium officinale were determined. The results showed that samples collected May February 32.89%-43.07% 25.77%-35.25%, respectively, while 2.81%-4.85% 7.90%-17.40%, respectively. They significantly different among families. content offspring could be improved by hybridization between parents with low high contents, hybrid vigor was obvious. Cross breeding...