MicroRNAs are widely dysregulated in various cancers and integrated into tumorigenic programs as either oncogenes or tumor suppressor genes. Here, we show that miR-26b, which is down-regulated human breast cancer specimens cell lines, impairs viability triggers apoptosis of MCF7 cells. SLC7A11 identified a direct target miR-26b its expression remarkably increased both lines clinical samples. Furthermore, silence mimics miR-26b-aroused impairment Our studies reveal protective role the...

The shikimic acid (SA) pathway is a fundamental route to synthesize aromatic building blocks for cell growth and metabolic processes, as well fermentative production of various compounds. Genes encoding enzymes SA are not continuous on genome they differently regulated. In this study, efforts were made construct genetic modules that regulated by same Ptac promoter. Firstly, aro genes [aroG (NCgl2098), aroB (NCgl1559), aroD (NCgl0408) aroE (NCgl1567)] from Corynebacterium glutamicum ribosome...

Abstract Background Oxaliplatin resistance usually leads to therapeutic failure and poor prognosis in colorectal cancer (CRC), while the underlying mechanisms are not yet fully understood. Metabolic reprogramming is strongly linked drug resistance, however, role mechanism of metabolic oxaliplatin remain unclear. Here, we aim explore functions purine metabolism on oxaliplatin-induced apoptosis CRC. Methods An oxaliplatin-resistant CRC cell line was generated, untargeted metabolomics analysis...

Ivorenolide A (1), a novel 18-membered macrolide featuring conjugated acetylenic bonds and five chiral centers, was isolated from Khaya ivorensis. The structure of 1 fully determined by spectroscopic analysis, single-crystal X-ray diffraction, bioinspired total synthesis. Both compound its synthetic enantiomer 2 showed potent selective immunosuppressive activity.

Abstract The cytoplasm of the eukaryotic cell is subdivided into distinct functional domains by presence a variety membrane-bound organelles. remaining aqueous space may be further partitioned regulated assembly discrete ribonucleoprotein (RNP) complexes that contain particular proteins and messenger RNAs. These RNP granules are conserved structures whose importance highlighted studies linking them to human disorders like amyotrophic lateral sclerosis. However, relatively little known about...

Specific locations of carbon-carbon double bonds (C═C) in lipids often play an essential role biological processes, and there has been a booming development C═C composition analysis by mass spectrometry. However, universal derivatization fragmentation pattern for the annotation positions is still challenging attractive. To expand this field lipidomics, flexible convenient N-tosylaziridination method was developed, with high efficiency, sensitivity, specificity. The very fast (15 s), numbers...

The protein carrier and encapsulation system based on polyelectrolytes plays crucial roles in drug research development. Traditional methods such as isothermal titration calorimetry molecular dynamics simulation have illuminated parts of this complex relationship. However, they fall short capturing the full picture interaction during carrier's fabrication loading dynamics. We propose a strategy article that combines ordered porous layer interferometry (OPLI) with electrophoretic light...

Abstract Renal fibrosis is a common pathological process in various chronic kidney diseases. The accumulation of senescent renal tubular epithelial cells (TECs) tissues plays an important role the development fibrosis. Eliminating TECs has been proven to effectively reduce Procyanidin C1 (PCC1) senolytic by specifically eliminating and extending its overall lifespan. However, whether PCC1 can alleviate unilateral ureteral obstruction (UUO)‐induced associated therapeutic mechanisms remains...

A novel plasmid-free engineered E. coli strain was developed to significantly increase l -cysteine yield, achieving a titer of 35.54 g L −1 in 5 bioreactor.

Ivorenolide B (1), an unprecedented 17-membered macrolide featuring conjugated acetylenic bonds and four chiral centers, was isolated from Khaya ivorensis. The structure of 1 fully determined by spectroscopic analysis total syntheses its most possible stereoisomers. Compound showed significant immunosuppressive activity.

Neuron connectivity and correct neural function largely depend on axonal integrity. Neurofilaments (NFs) constitute the main cytoskeletal network maintaining structural integrity of neurons exhibit dynamic changes during dendritic growth. However, mechanisms underlying development maintenance remain poorly understood. Here, we identify that multisynthetase complex p43 (MSC p43) is essential for NF assembly axon maintenance. The MSC protein was predominantly expressed in central interacted...

Shikimic acid (SA) is a key chiral starting molecule for the synthesis of neuramidase inhibitor GS4104 against viral influenza. Microbial production SA has been extensively investigated in Escherichia coli, and to less extent Bacillus subtilis. However, metabolic flux high SA-producing strains not explored. In this study, we constructed with genetic manipulation further determined 13C-labeling test B. subtilis strains.B. 1A474 had mutation kinase gene (aroI) accumulated 1.5 g/L SA....

Abstract The protein–protein interaction (PPI) is a basic strategy for life to operate. analysis of PPIs in multicellular organisms very important but extremely challenging because are particularly dynamic and variable among different development stages, tissues, cells, even organelles. Therefore, understanding PPI needs good resolution time space. More importantly, vivo be realized situ. Proximity-based biotinylation combined with mass spectrometry (MS) has emerged as powerful approach...

Eleven new mexicanolide-type limonoids, swietmanins A−I (1−4, 7−11), 2-hydroxy-3-O-isobutyrylproceranolide (5), 2-hydroxy-3-O-benzoylproceranolide (6), and a andirobin-type limonoid, swietmanin J (12), together with 19 known compounds were isolated from the fruits of Swietenia mahagoni. Their structures established on basis spectroscopic methods. These evaluated against small panel microorganisms.

This study was undertaken to explore the effects of trichostatin A (TSA), an inhibitor histone deacetylase, on viability, apoptosis, and invasiveness hypoxic rheumatoid arthritis fibroblast-like synoviocytes (RA FLSs). RA FLSs were exposed hypoxia for 24 h in presence or absence 2 μM TSA tested cell invasion, gene expression. The involvement phosphatidylinositol-3-kinase (PI3K)/Akt pathway checked. significantly inhibited viability induced apoptosis FLSs, compared vehicle control. blocked...

Cold shock proteins (Csps) enable organisms to acclimate and survive in cold environments the bacterial CspA family exerts protection via its RNA chaperone activity. However, most Archaea do not contain orthologs csp. TRAM, a conserved domain among modification ubiquitously distributed organisms, occurs as an individual protein archaeal phyla has structural similarity Csp proteins, yet biological functions remain unknown. Through physiological biochemical studies on four TRAM from adaptive...

Seven new limonoids, amotsangins A−G (1−7), two known and four sesquiterpenoids were isolated from the twigs leaves of Amoora tsangii. Their structures elucidated primarily on basis spectroscopic data.

Triple-negative breast cancer (TNBC) is a highly heterogeneous disease with different molecular subtypes. Although progress has been made, the identification of TNBC subtype-associated biomarkers still hindered by traditional RNA-seq or array technologies, since bulk data detected them usually have some non-disease tissue samples, they are confined to measure averaged properties whole tissues. To overcome these constraints and discover subtype-specific prognosis signatures (TSPSigs), we...

Treatment of non-small cell lung cancer (NSCLC) with drugs targeting the epidermal growth factor receptor (EGFR), e.g., gefitinib and erlotinib, will eventually fail because development secondary mutations such as T790M in EGFR. Strategies to overcome this resistance are therefore an urgent need. In study, we synthesized a dozen novel analogues evaluated their effects on L858R/T790M-EGFR harboring NSCLC cells, reported that one these mimetics, N-(2-bromo-5-(trifluoromethyl)...