Jinhua pig, a well-known Chinese indigenous breed, has evolved as pig breed with excellent meat quality, greater disease resistance and higher prolificacy. The reduction in the number of pigs over past years raised concerns about inbreeding. Runs homozygosity (ROH) along genome been applied to quantify individual autozygosity improve understanding inbreeding depression identify genes associated traits interest. Here, we investigated occurrence distribution ROH using next-generation...

ABSTRACT Gram-positive Streptomyces bacteria produce thousands of bioactive secondary metabolites, including antibiotics. To systematically investigate genes affecting metabolism, we developed a hyperactive transposase-based Tn 5 transposition system and employed it to mutagenize the model species coelicolor , leading identification 51,443 insertions. These insertions were distributed randomly along chromosome except for some preferred regions associated with relatively low GC content in...

Abstract Background Jianli pig, a renowned indigenous breed in China, has the characteristics of two-end black (TEB) coat color, excellent meat quality, strong adaptability and increased prolificacy. However, there is limited information available regarding genetic diversity, population structure genomic regions under selection pig. On other hand, mechanism TEB color remained largely unknown. Results In this study, whole genome resequencing 30 pigs within context 153 individuals representing...

The research on genetic characterization of Chinese indigenous pig breeds is essential to promote scientific conservation and sustainable development pigs. Here, we systematically surveyed the genomes 75 unrelated Diannan small-ear (DSE) pigs that were from 3 diverse regions (Yingjiang County, Jinping County Sipsongpanna in Yunnan Province) describe their population structure, diversity, inbreeding coefficient, selection signatures. First, these individuals sequenced genotyped following...

Laiwu pigs, distinguished by their high intramuscular fat of 7–9%, is an indigenous pig breed China, and recent studies also found that pigs showed resistance to Porcine circovirus type 2. However, with the introduction commercial varieties, population has declined, some lineages have disappeared, which could result in inbreeding. Runs homozygosity (ROH) can be used as a good measure individual inbreeding status normally detect selection signatures so map candidate genes associated...

Abstract Motivation Small non-coding RNAs (ncRNAs), especially microRNAs (miRNAs) and piwi-interacting (piRNAs), play key roles in many biological processes. However, only a few tools can be used to develop the optimal primer or probe design for expression profile of small ncRNAs. Here, we developed sRNAPrimerDB, first automated designing query web service Results The online module sRNAPrimerDB is composed algorithms quality evaluation polymerase chain reaction (PCR) primer. Five types...

Chinese indigenous pigs in the Taihu Lake region are well known for their high fecundity and other excellent characteristics. To better understand characteristics of these breeds this area as to provide government breeders molecular basis formulating a reasonable conservation policy, we explored structure haplotype blocks genetic diversity 7 populations which is relevant management important resources using next-generation sequencing data. In study, total 131 300 single-nucleotide...

The primary purpose of the current study was to assess genetic diversity, runs homozygosity (ROH) and ROH islands in a Chinese composite pig explore hotspot regions for traces selection. First, we estimated length, number, frequency 262 Xidu black pigs using Porcine SNP50 BeadChip compared estimates inbreeding coefficients, which were calculated based on ROHs (F ) HOM ). Our result shows that total 7,248 exceeding 1Mb detected pigs. In addition, Sus scrofa chromosome (SSC) 8 SSC10,...

Muscle growth and development are important aspects of chicken meat production, but the underlying regulatory mechanisms remain unclear need further exploration. CRISPR has been used for gene editing to study function in mice, less done chick muscles. To verify whether postnatal could be achieved muscles determine transcriptomic changes, we knocked out Myostatin (MSTN), a potential inhibitor muscle development, chicks performed transcriptome analysis on knock-out (KO) wild-type (WT) at two...

A marine prosthecate bacterium, designated strain P-1 km-3(T), was isolated from deep seawater the Pacific. Cells of km-3(T) were Gram-stain-negative, aerobic, catalase- and oxidase-positive, dimorphic rods with a single polar prostheca or flagellum. The hydrolysed gelatin grew at 6-40 °C (optimum, 30 °C) 0.5-12% (w/v) NaCl 2%). Phylogenetic analysis 16S rRNA gene sequences revealed that belonged to family Hyphomonadaceae in class Alphaproteobacteria represented separate lineage, located...

Myostatin (MSTN), a member of the transforming growth factor-β superfamily, plays crucial negative role in muscle growth. MSTN mutations or inhibitions can dramatically increase mass most mammal species. Previously, we generated transgenic mouse model hypertrophy via expression N-terminal propeptide cDNA under control skeletal muscle-specific MLC1 promoter. Here, compare mRNA profiles between mice and wild-type littermate controls with high-throughput RNA sequencing method. The results show...

Abstract Background China is the country with most abundant swine genetic resources in world. Through thousands of years domestication and natural selection, pigs have developed unique characteristics. Finding characteristics modules each breed an essential part their precise conservation. Results In this study, we used partial least squares method to identify significant specific SNPs 19 local Chinese pig breeds 5 Western breeds. A total 37,514 ( p < 0.01) were obtained from these...

Ibogaine (IBO) is an indole alkaloid from the West African shrub, Tabernanthe iboga. It structurally related to harmaline, and both these compounds are rigid analogs of melatonin. IBO has psychoactive stimulant properties. In single-blind trials with humans, it ameliorated withdrawal symptoms interrupted addiction process. However, also produced neurodegeneration Purkinje cells gliosis Bergmann astrocytes in cerebella rats given even a single dose (100 mg/kg, ip). Here, we treated (n = 6 per...

Streptomyces species are important antibiotic-producing organisms that tightly regulate their antibiotic production. Actinorhodin is a typical produced by the model actinomycete coelicolor To discover regulators of actinorhodin production, we constructed library 50,000 independent mutants with hyperactive Tn5 transposase-based transposition systems. Five hundred fifty-one genes were found to influence production in 988 individual mutants. Genetic complementation suggested most insertions...

DNA methylation plays a critical role in regulating gene expression during testicular development. However, few studies report on candidate genes related to the regulation of porcine This study examined differentially expressed (DEGs) and their levels tissues from pigs at 60 days age (60 d) 180 (180 using RNA-Seq whole genome bisulfite sequencing (WGBS). It was determined that primarily occurs cytosine-guanine (CG) context, analysis identified 106,282 methylated regions (DMRs) corresponding...

Abstract A recessive epistatic genic male sterility (REGMS) two‐type line, 9012AB, has been used for rapeseed hybrid seed production in China. The of 9012AB is controlled by two duplicate sterile genes ( ms1 and ms2 ) interacting with one suppressor gene esp ). Homozygosity at the locus espesp suppresses expression trait homozygous ms1ms1ms2 plants. In this study, we a combination bulked segregant analyses amplified fragment length polymorphism (AFLP) to identify markers linked BC 1...

The aim of this study was to detect evidence for signatures recent selection in the Jinhua pig genome. These results can be useful better understand regions under pigs and might shed some lights on groups genes that control production traits. In present study, we performed extended haplotype homozygosity (EHH) tests identify significant core 202 pigs. A total 26 161 spanning 636.42 Mb were identified, which occupied approximately 28% genome across all autosomes, 1 158 (P<0.01) haplotypes...

Objective Porcine respiratory disease is one of the most important health problems causing significant economic losses. To understand genetic basis for susceptibility to swine enzootic pneumonia (EP) in pigs, we detected 102,809 single nucleotide polymorphisms a total 249 individuals based on genome-wide sequencing data. Methods Genome comparison EP three pig breeds (Jinhua, Erhualian, and Meishan) with two western lines that are considered more resistant (Duroc Landrace) using...

Summary Anqing six‐end‐white (AQ) pig performs well on resistance to coarse fodder and disease, reproduction meat quality, offering high potential for exploitation. Environmental conditions strict selections from local farmers have cultivated the AQ be an outstanding unique breed. Thus we aim detect genetic positive selection signatures within population explore underlying mechanisms. A relative extended haplotype homozygosity (REHH) test was performed in of 79 pigs seek evidence...

Abstract Over the past decade, polima cytoplasmic male sterility ( pol CMS) three‐line and two‐line systems have been developed for production of hybrid seed in Brassica napus oilseed rape China. The discovery novel CMS restorer line FL‐204 is described here. It restores fertility plants system, but can only be carried out under autumn sowing Wuhan south China moderate temperatures at flowering. cannot used as a northwestern (Gansu) spring conditions, because there it more or less sterile...

Feed efficiency (FE) is a very important trait in livestock industry. Identification of the candidate genes could be benefit for improvement FE trait. Mouse used as model many studies mammals. In this study, related to and coat color were identified using C57BL/6J (C57) × Kunming (KM) F2 mouse population. GWAS results showed that 61 2 SNPs genome-wise suggestive significantly associated with feed conversion ratio (FCR) intake (FI) traits, respectively. Moreover, Erbin, Msrb2, Ptf1a, Fgf10...

Production of secondary metabolites is tightly regulated by transcriptional regulators in Streptomyces. Many have been identified gene disruption Streptomyces; however, there are still unknown regulatory genes yet to be revealed due silence under laboratory culture conditions. In present study, a T7 expression system was used identify novel deletion and constitutive-expression nine candidates. We reported that the MarR family regulator MapR (SCO2398) resulted increase actinorhodin (ACT)...

Nowadays, breed conservation has entered the genomics era and it is imperative to develop novel theory design breeding schemes of populations by using genomic information. The genome-wide markers have been regarded as a useful strategy maintain genetic diversity. However, SNPs optimize diversity might not be optimal for some specific loci associated with specific-traits. Using sequencing data conserved population Saba pig breed, we demonstrated that program designed result in loss...