A5062039093- Ultrasound and Hyperthermia Applications
- Photoacoustic and Ultrasonic Imaging
- Glioma Diagnosis and Treatment
- Spectroscopy Techniques in Biomedical and Chemical Research
- Alzheimer's disease research and treatments
- Ultrasound Imaging and Elastography
- Advanced MRI Techniques and Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- 3D Printing in Biomedical Research
- Neuroblastoma Research and Treatments
- PARP inhibition in cancer therapy
- Health Systems, Economic Evaluations, Quality of Life
- Microscopic Colitis
- Intracerebral and Subarachnoid Hemorrhage Research
- Cerebrovascular and genetic disorders
- Ultrasound and Cavitation Phenomena
- Nanoparticle-Based Drug Delivery
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Bio-sensing Technologies
- Electrical and Bioimpedance Tomography
- Nanoplatforms for cancer theranostics
- Cell Adhesion Molecules Research
- Inflammatory Bowel Disease
- Delphi Technique in Research
- Advanced Neuroimaging Techniques and Applications
Princess Máxima Center
2019-2023
University Medical Center Utrecht
2012-2022
Leiden University Medical Center
2019-2021
Heidelberg University
2019
University Hospital Heidelberg
2019
Association François Aupetit
2017
Centre National de la Recherche Scientifique
2010-2012
ESPCI Paris
2010
Inserm
2010
Institut Langevin
2010
Arterial spin labeling (ASL)‐MRI can noninvasively map cerebral blood flow (CBF) and cerebrovascular reactivity (CVR), potential biomarkers of cognitive impairment dementia. Mouse models disease are frequently used in translational MRI studies, which commonly performed under anesthesia. Understanding the influence specific anesthesia protocol on measured parameters is important for accurate interpretation hemodynamic studies with mice. Isoflurane a anesthetic vasodilative properties. Here,...
Microbubbles (MBs) in combination with ultrasound (US) can enhance cell membrane permeability, and have the potential to facilitate cellular uptake of hydrophilic molecules. However, exact mechanism behind US- MB-mediated intracellular delivery still remains be fully understood. Among proposed mechanisms are formation transient pores endocytosis stimulation. In our study, we investigated whether is involved small Dynamic fluorescence microscopy was used investigate effects inhibitors on...
Cancer immunotherapy has transformed the landscape of adult cancer treatment and holds a great promise to treat paediatric malignancies. However, in vitro test coculture systems evaluate efficacy immunotherapies on representative tumour models are lacking. Here, we describe detailed procedure for establishment an ex vivo system organoids immune cells that enables assessment different approaches organoids. We provide step-by-step protocol efficient generation patient-derived diffuse intrinsic...
Diffuse midline glioma H3K27-altered (DMG) is an aggressive, inoperable, predominantly paediatric brain tumour. Treatment strategies are limited, resulting in a median survival of only 11 months. Currently, radiotherapy (RT), often combined with temozolomide, considered the standard care but remains palliative, highlighting urgency for new therapies. Radiosensitisation by olaparib, inhibitor PARP1 and subsequently PAR-synthesis, promising treatment option. We assessed whether inhibition...
Abstract CADASIL is a NOTCH3 -associated cerebral small vessel disease. A pathological ultrastructural disease hallmark the presence of NOTCH3-protein containing deposits called granular osmiophilic material (GOM), in arteries. How these GOM develop over time and what their role progression largely unknown. Here, we studied humanized transgenic Arg182Cys mice, compared them to patient material, determined whether mice are associated with functional phenotype. We found that not static, but...
Impaired cerebrovascular function is an early biomarker for cerebral amyloid angiopathy (CAA), a neurovascular disease characterized by amyloid-β accumulation in the vasculature, leading to stroke and dementia. The transgenic Swedish Dutch Iowa (Tg-SwDI) mouse model develops microvascular deposits, but whether this leads similar functional impairments incompletely understood. We assessed longitudinally Tg-SwDI mice with arterial spin labeling (ASL)-magnetic resonance imaging (MRI) laser...
Diffuse midline glioma (DMG) is an aggressive brain tumour with high mortality and limited clinical therapeutic options. Although in vitro research has shown the effectiveness of medication, successful translation to clinic remains elusive. A literature search highlighted variability lack standardisation protocols applied for establishing commonly used HSJD-DIPG-007 patient-derived xenograft (PDX) model, based on animal host, injection location, number cells inoculated, volume, suspension...
Quantitative Increasing number of patients is affected by chronic kidney disease (CKD) world while with ensuing end-stage renal (ESRD). A change in global approach to CKD from costly treatment ESRD more aggressive primary and secondary prevention imperative. This improvement needs: a better understanding mechanisms underlying scarring, development specific therapies slow the progression modern diagnostic tools characterize these processes. Number underestimates has probably exceeded factor...
The combination of ultrasound and microbubbles can facilitate cellular uptake (model) drugs via transient permeabilization the cell membrane. By using fluorescent molecules, this process be studied conveniently with confocal fluorescence microscopy. This study aimed to investigate relation between intensity increase intercalating model drugs.SYTOX Green, an dye that displays >500-fold enhancement upon binding nucleic acids, was used as a drug for ultrasound-induced uptake. SYTOX Green...
In solid tumors, rapid local intravascular release of anticancer agents, e.g., doxorubicin (DOX), from thermosensitive liposomes (TSLs) can be an option to overcome poor extravasation drug nanocarriers. The driving force DOX penetration is the concentration gradient between vascular compartment and tumor interstitium. this feasibility study, we used fibered confocal fluorescence microscopy (FCFM) monitor in real-time interstitium a subcutaneous after its TSLs, Thermodox®. Cell uptake...
We have previously demonstrated the feasibility of monitoring ultrasound-mediated uptake a hydrophilic model drug in real time with dynamic confocal fluorescence microscopy. In this study, we evaluate and correct impact photobleaching to improve accuracy pharmacokinetic parameter estimates.
The blood-brain barrier (BBB) has been a major hurdle for the treatment of various brain diseases. Endothelial cells, connected by tight junctions, form physiological preventing large molecules (>500 Da) from entering tissue. Microbubble-mediated focused ultrasound (FUS) can be used to induce transient local BBB opening, allowing larger drugs enter parenchyma. In addition large-scale clinical devices translation, preclinical research therapy response assessment drug candidates requires...
Background: Value-based healthcare aims to achieve the best possible health outcomes for lowest cost. Key its success involves measuring that matter most patients. Currently inflammatory bowel disease (IBD), registries and clinical trials lack a unifying set of well-defined outcomes, making comparisons between populations difficult. Our goal was develop minimum Standard Set patient-centered IBD provide common language can be tracked systematically in variety settings. Methods: An...
ABSTRACT Vascular dysfunction is increasingly recognized to play a role in the development of Alzheimer’s disease (AD). The relation between vascular and neuropathological amyloid β accumulation characteristic for AD however unclear. limited resolution vivo imaging techniques, intricate 3D structure microvasculature different co-occurring types patients hamper studying this patients. Here, we therefore employed APP/PS1 mouse model, which develops parenchymal plaques, study effect plaques on...