Cell loss due to apoptosis induced by oxidative stress is a major hurdle for endothelial progenitor cells (EPCs)‐based therapy. Sirtuin 1 (SIRT1) plays important roles in many pathophysiological processes deacetylating various substrates, including forkhead transcription factor (FOXO). However, after deacetylation, the fate of FOXO protein remains be explored. In present study, we investigated whether SIRT1 exerted protective effect on hydrogen peroxide (H 2 O )‐induced EPCs and, if so, what...

Abstract Background Mesenchymal stem cells (MSCs) play a crucial role in maintaining the dynamic balance of bone metabolism. Melatonin may have regulatory effect on metabolism by regulating lineage commitment and differentiation signalling pathways MSCs. Among BMP families, osteogenesis BMP9 is considered to be one strongest Here, we explored whether melatonin act synergistically MSC osteogenic differentiation. Methods The C3H10T1/2 function induced synergizes with BMP9, as detected...

Endothelial‐mesenchymal transition (EndMT) plays a pivotal role in organ fibrosis. This study examined the effect of SIRT1 on transforming growth factor beta (TGF‐β)‐induced EndMT human endothelial cells (ECs) and its probable molecular mechanism. We assessed by immunofluorescence staining, quantitative real‐time polymerase chain reaction, Western blotting, migration invasion assays. Adenovirus was used to overexpress or knockdown ECs. The regulatory relationship between Smad4 analyzed...

HSP70 overexpression has a remedying effect in neurodegenerative diseases. In Alzheimer's disease (AD), the suppressive effects of on AD-related phenotypes and underlying mechanisms are unknown. current study, geranylgeranylacetone (GGA), non-toxic inducer heat shock protein (HSP)-70 expression, cognitive function other pathological were evaluated APP/PS1 mice. It was observed that all doses orally administered GGA (200, 400, 800 mg/kg/day) improved deficit (P<0.05) lowered levels amyloid-β...

Cellular aging may be associated with epigenetics. Methyl-CpG-binding protein 2 (MeCP2) and sirtuin 1 (SIRT1) are two important epigenetic factors. Our former work demonstrated that MeCP2 expression increased SIRT1 decreased in senescent endothelial progenitor cells (EPCs). This article aims to reveal the regulation caused by EPCs discuss its mechanism. Tube formation assay cell apoptosis detection were used evaluate function of induced overexpression. Western blot analysis was testify...

Background Force platform training with functional electric stimulation aimed at improving balance may be effective in fall prevention for older adults. Aim of the study is to evaluate effects visual-feedback-based force on and Methods A single-centre, unblinded, randomized controlled trial was conducted. One hundred twenty adults were randomly allocated two groups: control group ( n = 60, one-leg standing exercise, 12 min/d) or intervention stimulation, min/d). The provided 15 days a month...

Endothelial dysfunction is involved in the pathogenesis of many cardiovascular diseases such as atherosclerosis. progenitor cells (EPCs) have been considered to be great significance therapeutic angiogenesis. Furthermore, Forkhead box O (FOXO) transcription factors are known important regulators cell cycle. Therefore, we investigated effects changes FOXO3a activity on proliferation and cycle regulatory proteins EPCs. The constructed recombinant adenovirus vectors Ad-TM (triple...

Abstract: Biscarbamate cross-linked polyethylenimine derivative (PEI-Et) has been reported as a novel nonviral vector for efficient and safe gene transfer in our previous work. However, it had no cell-specificity. To achieve specific delivery of genes to hepatocytes, galactosylated poly(ethylene glycol)-graft-polyethylenimine (GPE) was prepared through modification PEI-Et with glycol) lactobionic acid, bearing galactose group hepatocyte-targeting moiety. The composition GPE characterized by...

Background/Aims: Heart failure is the end result of various kinds cardiovascular diseases. It has a high rate morbidity and mortality. This article aims to determine effect MeCP2, key epigenetic regulator, on heart failure. Methods: The genes associated with were selected analyzed using Gene Ontology (GO) term analysis protein-protein interaction (PPI) network analysis. Significantly up- or downregulated in animal model identified, that had same opposite alteration trends as MeCP2 also...

Autophagy is an evolutionarily conserved intracellular degradation mechanism in which cell constituents are phagocytosed to maintain cellular homeostasis. Forkhead box O 3a (FoxO3a) promotes autophagy protect cells from environmental stress. Methylated CpG binding protein 2 (MeCP2) a nuclear that binds DNA and represses transcription. However, the interplay between FoxO3a MeCP2 underlying endothelial progenitor (EPC) function not fully understood.In EPCs, overexpression attenuated...

Therapeutic neovascularization is a strategy to promote blood vessel growth and improve flow, which critical tissue repair regeneration in ischemic diseases. Here, we investigated the role of endothelial progenitor cell - derived exosomes (EPC-Exos) therapeutic clarified mechanism hsa_circ_0093884 EPC-Exos mediated neovascularization. Injection improved mouse hindlimb perfusion, promoted angiogenesis Matrigel plugs skin wound healing. In vitro coculture with HUVEC proliferation, angiogenic...

Acidic fibroblast growth factor (FGF1) has been suggested to enhance the functional activities of endothelial progenitor cells (EPCs). The Forkhead homeobox type O transcription factors (FOXOs), a key substrate survival kinase Akt, play important roles in regulation various cellular processes. We previously have shown that FOXO3a is main subtype FOXOs expressed EPCs. Here, we aim determine whether FGF1 promotes EPC function through Akt/FOXO3a pathway. Human peripheral blood derived EPCs were...

Gene silencing by RNA interference (RNAi) is a promising approach for gene therapy. However, up to today, it still major challenge find safe and efficient non-viral vectors. Previously, we reported PEI-Bu, small molecular weight PEI derivative, as an carrier. like many PEI-based polymers, PEI-Bu was too toxic. In order reduce cytotoxicity while maintain or even enhance transfecion efficiency, modified with poly(ethylene glycol) (PEG) obtain PEG-Bu, used delivery theraputic short hairpin...

// Chunli Wang 1,* , Fei Zhen Li 1 Liya Huang Qing Cao and Shuyan Chen Department of Geriatrics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School Medicine, Shanghai, China * These authors have contributed equally this work Correspondence to: Chen, email: Keywords : EPCs, senescence, MeCP2, SIRT1, Gerotarget Received April 20, 2017 Accepted August 26, Published September 16, Abstract Aging endothelial progenitor cells (EPCs) exhibit functional impairment in terms...