A5008261341- Forensic and Genetic Research
- Molecular Biology Techniques and Applications
- Environmental DNA in Biodiversity Studies
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Epigenetics and DNA Methylation
- Chemical synthesis and alkaloids
- Bone Metabolism and Diseases
- Wnt/β-catenin signaling in development and cancer
- Salivary Gland Disorders and Functions
- Fungal and yeast genetics research
- Oral microbiology and periodontitis research
- Identification and Quantification in Food
- Retinoids in leukemia and cellular processes
- Ginseng Biological Effects and Applications
- Acne and Rosacea Treatments and Effects
- Genomic variations and chromosomal abnormalities
- Computational and Text Analysis Methods
- Cancer-related gene regulation
- RNA Research and Splicing
- Demographic Trends and Gender Preferences
- Enzyme Catalysis and Immobilization
- TGF-β signaling in diseases
- Catalytic Cross-Coupling Reactions
- Diverse Topics in Contemporary Research
Seoul National University
2009-2025
National Forensic Institute
2016-2025
Seoul Institute
2025
Korea Advanced Institute of Science and Technology
2015
Korea University
2011-2014
Korea Research Institute of Bioscience and Biotechnology
2014
Konkuk University
2010
Seoul National University Hospital
2009
Korea Institute of Radiological and Medical Sciences
2008
The high price of petroleum-based diesel fuel has led to the development alternative fuels, such as ethanol. Saccharomyces cerevisiae was metabolically engineered utilize glycerol a substrate for ethanol production. For synthesis fatty acid ethyl esters (FAEEs) by S. that substrate, heterologous expression an unspecific acyltransferase from Acinetobacter baylyi with utilizing genes established. As result, YPH499 (pGcyaDak, pGupWs-DgaTCas) strain produced 0.24 g/L FAEEs using endogenous...
Surfactin is a biological surfactant with numerous potential applications. In this study, Bacillus subtilis was engineered to improve surfactin production by the activation of two competence-stimulating pheromones, ComX and competence sporulation factor (CSF) stimulate transcription srfA operon. Both signaling factors, encoded comX phrC, were successfully overexpressed subsequently increased production. produced strains showed functional groups similar commercially available analyzed via...
Osteoporosis is a systemic skeletal disorder characterized by reduced bone mineral density (BMD) and increased risk of fracture. We studied the effects transplantation mesenchymal stem cells (MSCs) overexpressing receptor activator nuclear factor-kappaB (RANK)-Fc CXC chemokine receptor-4 (CXCR4) using retrovirus on ovariectomy (OVX)-induced loss in mice. Ten-week-old adult female C57BL/6 mice were divided into six groups as follows: Sham-operated treated with phosphate-buffered saline (PBS)...
Osteoblasts are derived from mesenchymal progenitors. Differentiation to osteoblasts and adipocytes is reciprocally regulated. Transcriptional coactivator with a PDZ-binding motif (TAZ) transcriptional that induces differentiation of cells into while blocking adipocytes. To investigate the role TAZ on bone metabolism in vivo, we generated transgenic mice overexpress under control procollagen type 1 promoter (Col1-TAZ). Whole body mineral density (BMD) 6- 19-week-old Col-TAZ was 4% 7% higher...
This study developed a new method for forensic saliva identification using three oral bacteria, Streptococcus salivarius, sanguinis, and Neisseria subflava, combined with real-time polymerase chain reaction (RT-PCR) system we called OB mRT-PCR. Analytical sensitivity results showed that the target bacteria were amplified at 102-107 copies/reaction, analytical specificity was assessed 24 other viruses, protozoa. To evaluate mRT-PCR kit applications, from 140 Korean individuals tested, least...
ABSTRACT Estimating age at death narrows the pool of potential donors in mass disasters and criminal investigations. In this study, we developed a capillary electrophoresis‐based SNaPshot assay for prediction costal cartilage used it to analyze DNA methylation 11 CpG sites across six genes 136 samples from deceased Koreans aged 28–84 years. To develop predictive model, levels these training set 83 were analyzed using multivariate linear regression five ways. We then compared performance...
Abstract PPARγ has critical role in the differentiation of mesenchymal stem cells into adipocytes while suppressing osteoblastic differentiation. We generated transgenic mice that overexpress specifically osteoblasts under control a 2.3-kb procollagen type 1 promoter (Col.1-PPARγ). Bone mineral density (BMD) 6- to 14-week-old Col.1 − male was 8% 10% lower than their wild-type littermates, whereas no difference noticed Col.1-PPARγ female mice. exhibited decreased bone volume (45%), trabecular...
Triacylglycerol (TAG) is a microbial oil feedstock for biodiesel production that uses an inexpensive substrate, such as glycerol. Here, we demonstrated the overproduction of TAG from glycerol in engineered Saccharomyces cerevisiae via glycerol-3-phosphate (G3P) pathway by overexpressing major synthesis. The G3P accumulation was increased 2.4-fold with utilization gained overexpression kinase (GUT1). By diacylglycerol acyltransferase (DGA1) and phospholipid (LRO1), YPH499 (pGutDgaLro1) strain...
The DNA intelligence tool, methylation-based age prediction, can help identify disaster victims and suspects in criminal investigations. In this study, we developed a costal cartilage-based prediction tool that uses massive parallel sequencing (MPS) of age-associated methylation markers. Costal cartilage samples were obtained from 85 deceased Koreans, aged between 26 89 years. An MPS library was prepared using two rounds multiplex polymerase chain reaction nine genes (TMEM51, MIR29B2CHG,...
Background & Aims Accumulating evidence suggests that retinol and its metabolites are closely associated with liver fibrogenesis. Recently, we demonstrated genetic ablation of alcohol dehydrogenase 3 (ADH3), a metabolizing gene is expressed in hepatic stellate cells (HSCs) natural killer (NK) cells, attenuated fibrosis mice. In the current study, investigated whether pharmacological ADH3 has therapeutic effects on experimentally induced Methods Liver was by intraperitoneal injections carbon...
The analytical study of intracellular (IC) metabolites has developed with advances in chromatography-linked mass spectrometry and fast sampling procedures. We applied the IC metabolite analysis to characterize role GCY1 glycerol (GLY) catabolic pathway Saccharomyces cerevisiae.Strains disrupted or overexpressing GLY genes such as GCY1, DAK1 DAK2 were constructed. strains cultivated under different aeration conditions quickly quenched using a novel rapid port. concentrations GLY,...