A5101828479- Alzheimer's disease research and treatments
- Neurological Disease Mechanisms and Treatments
- Dementia and Cognitive Impairment Research
- Pancreatic function and diabetes
- Cannabis and Cannabinoid Research
- Neuroscience and Neuropharmacology Research
- Geriatric Care and Nursing Homes
- S100 Proteins and Annexins
- Neurological Disorders and Treatments
- Cerebrovascular and genetic disorders
- Pregnancy and preeclampsia studies
- Synthesis and Biological Evaluation
- Prenatal Screening and Diagnostics
- Cerebrovascular and Carotid Artery Diseases
- Mitochondrial Function and Pathology
- Retinal Diseases and Treatments
- Cancer, Hypoxia, and Metabolism
- Sphingolipid Metabolism and Signaling
- Acute Ischemic Stroke Management
- Ocular and Laser Science Research
- Gestational Trophoblastic Disease Studies
- Glaucoma and retinal disorders
- Cardiac electrophysiology and arrhythmias
- Enzyme Catalysis and Immobilization
- Natural Antidiabetic Agents Studies
Yamaguchi University
2014-2022
Osaka Women's and Children's Hospital
2017-2022
Tokushima University
1998-2021
Kyoto University
1993-2020
Shinshu University
2019
Osaka Red Cross Hospital
2017
National Cerebral and Cardiovascular Center
2013-2016
Kansai Electric Power (Japan)
2016
Keio University Hospital
2016
Newcastle Hospitals - Campus for Ageing and Vitality
2015
Cortical microinfarcts (CMIs) observed in brains of patients with Alzheimer's disease tend to be located close vessels afflicted cerebral amyloid angiopathy (CAA). CMIs are preferentially distributed the arterial borderzone, an area most vulnerable hypoperfusion. However, causal association between CAA and remains elucidated. This study consists two parts: (1) observational using postmortem human (n = 31) determine CMIs, (2) experimental whether hypoperfusion worsens induces a mouse model....
Glucose transporter 1 (GLUT1) plays an important role in the transport of glucose placenta. During early pregnancy, placentation occurs a relatively hypoxic environment that is essential for appropriate embryonic development, and GLUT1 expression enhanced response to oxygen deficiency Hypoxia-inducible factor-1 (HIF-1)alpha involved induction other cells. The present study was designed test whether HIF-1alpha hypoxia-induced activation using trophoblast-derived human BeWo rat Rcho-1 cells as...
White matter hyperintensities as seen on brain T 2 -weighted magnetic resonance imaging are associated with varying degrees of cognitive dysfunction in stroke, cerebral small vessel disease and dementia. The pathophysiological mechanisms within the white accounting for remain unclear. With hypothesis that gliovascular interactions impaired subjects high burdens hyperintensities, we performed clinicopathological studies post-stroke survivors, who had exhibited greater frontal volumes...
Brain amyloidosis is a key feature of Alzheimer's disease (AD). It also incorporates cerebrovascular amyloid β (Aβ) in the form cerebral angiopathy (CAA) involving neurovascular dysfunction. We have recently shown by retrospective analysis that patients with mild cognitive impairment receiving vasoactive drug cilostazol, selective inhibitor phosphodiesterase (PDE) III, exhibit significantly reduced decline. Here, we tested whether cilostazol protects against disruption unit and facilitates...
A novel series of rhodanin (RH) and thiohydantoin (TH) derivatives were designed synthesized for detecting tau pathology in the brains patients with Alzheimer's disease (AD). In experiments vitro using β-amyloid (Aβ) aggregates, TH derivative, TH2, showed high specific binding to aggregates. hippocampal sections obtained from AD patients, TH2 intensely stained neurofibrillary tangles. normal mice, [125I]TH2 good uptake (1.54%ID/g, 2 min postinjection) into a rapid washout (0.25%ID/g, 60...
Background and Purpose Cognitive impairment resulting from cerebrovascular insufficiency has been termed vascular cognitive impairment, is generally accepted to be distinct Alzheimer's disease a neurodegenerative process. However, it clear that this simple dichotomy may need revision in light of the apparent occurrence several shared features between impairment. Nevertheless, still remains largely unknown whether burden vascular- Alzheimer-type neuropathology are independent or...
Cortical microinfarcts are reported in Alzheimer's disease, but not subcortical vascular dementia; the disease specificity of cortical therefore remains unclear. The distribution (n = 8) and dementia 6) was analyzed. were frequently detected whereas they rarely observed dementia. In present predominantly occipital lobe, area predilection for amyloid angiopathy, also borderzone. invariably located very close to beta-deposited vessels with intercellular adhesion molecule-1 expression. These...
Silent information regulator 2 homolog 1 (SIRT1) is a protein deacetylase that has been reported to suppress neurodegenerative and cardiovascular pathologies in model organisms. We have recently SIRT1 overexpression preserves cerebral blood flow (CBF) after bilateral common carotid artery stenosis (∼50% stenosis) by the deacetylation of endothelial nitric oxide synthase. This study was designed determine whether expression would be effective more severe ischemia caused occlusion (BCAO) vivo....
Abstract During early pregnancy, cytotrophoblast cells differentiate into extravillous trophoblast (EVT) and invade the uterine spiral arteries. This physiological process is essential for development of maternal-fetal circulation. Because EVT are exposed to a low-oxygen environment during this process, we investigated role hypoxia in mechanism that regulates invasive behavior cells. Real-time PCR immunofluorescent analysis were performed investigate how influences expression E-cadherin...
Two radiofluoro-pegylated phenylbenzoxazole derivatives, 4-(5-(2-(2-(2-[(18)F]fluoroethoxy)ethoxy)ethoxy)benzo[d]oxazol-2-yl)-N-methylaniline ([(18)F]24) and 4-(5-(2-(2-(2-[(18)F]fluoroethoxy)ethoxy)ethoxy)benzo[d]oxazol-2-yl)-N,N-dimethylaniline ([(18)F]32), were synthesized evaluated as probes for imaging cerebral β-amyloid (Aβ) plaques in living brain tissue by PET. [(18)F]24 [(18)F]32 displayed high affinity Aβ(1-42) aggregates (K(i) = 9.3 3.9 nM, respectively). In vitro autoradiography...
This paper describes the synthesis and biological evaluation of novel phenyldiazenyl benzothiazole (PDB) derivatives as probes for imaging neurofibrillary tangles (NFTs) in patients with Alzheimer's disease (AD). We successfully synthesized three PDB using a diazo coupling reaction. In binding experiments vitro, compounds displayed higher affinity tau aggregates than Aβ aggregates. fluorescent staining AD brain sections, 9 visualized NFTs clearly. No-carrier-added radioiodinated were...
In order to explore novel tau-imaging agents that can selectively detect neurofibrillary tangles in Alzheimer's disease (AD) brains, we designed and synthesized a series of heterocyclic phenylethenyl (3-pyridinyl)ethenyl derivatives with or without dimethyl amino group. vitro autoradiography using AD brain sections, all radioiodinated ligands group bound Aβ deposits the sections. contrast, showed different patterns radioactivity accumulation sections depending on kind heterocycle contained...
Imaging of β-amyloid (Aβ) plaques in the brain may facilitate diagnosis cerebral β-amyloidosis, risk prediction Alzheimer's disease (AD), and effectiveness anti-amyloid therapies. The purpose this study was to evaluate novel 123I-labeled pyridyl benzofuran derivatives as SPECT probes for Aβ imaging. formation a backbone accomplished by Suzuki coupling. [123I/125I]-labeled were readily prepared an iododestannylation reaction. In vitro binding assays carried out using Aβ(1–42) aggregates...
N-acylethanolamines (NAEs), a class of lipid mediators, are produced from N-acyl-phosphatidylethanolamine (NAPE) by several pathways, including the direct release NAPE-specific phospholipase D (NAPE-PLD) or multistep pathway via sn-glycero-3-phospho-N-acylethanolamine (Gp-NAE). Using liquid chromatography-tandem mass spectrometry, we compared peripheral tissue levels NAPE, Gp-NAE and NAE in NAPE-PLD-deficient (NAPE-PLD-/-) wild type (WT) mice. NAPE-PLD was suggested to play major role NAPE...
During early pregnancy, the invasion of trophoblast cells into decidua uterus is one essential steps in appropriate placentation. In this period, are exposed to a relatively low-oxygen environment. The c-met protooncogene product (Met), which high-affinity receptor for hepatocyte growth factor, plays an important role controlling many types cells. present study was designed investigate effect tension on Met expression and invasiveness isolated from early-stage human placenta...
We synthesized and evaluated (E)-4-((6-(2-(2-(2-fluoroethoxy)ethoxy)ethoxy)benzo[d]thiazol-2-yl)diazenyl)-N,N-dimethylaniline (FPPDB) as a probe for the imaging of neurofibrillary tangles (NFTs) in patients with Alzheimer's disease (AD). In assays using thioflavin S (ThS) competitive ligand, FPPDB competed ThS well showed high affinity both tau Aβ1-42 aggregates (K i = 13.0 20.0 nM, respectively). The results saturation binding also verified that bound to d 44.8 nM B max 45.8 pmol/nmol...
Facilitative glucose transporter-1 (GLUT1) is abundant in trophoblast cells and responsible for transport the placenta. However, change GLUT expression human placenta upon differentiation remains to be clarified. Therefore, we first examined localization of GLUT1 GLUT3 using first-trimester chorionic villi. We found that were mainly localized syncytiotrophoblast cytotrophoblast respectively. analyzed whether placental changes during a choriocarcinoma (BeWo) cell line which known show...
Connecting RNA-Modifying Similarities of TDP-43, FUS, and SOD1 with MicroRNA Dysregulation Amidst A Renewed Network Perspective Amyotrophic Lateral Sclerosis Proteinopathy,