A5100320331- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Power Systems and Renewable Energy
- Streptococcal Infections and Treatments
- Viral-associated cancers and disorders
- Respiratory viral infections research
- RNA Interference and Gene Delivery
- Cytomegalovirus and herpesvirus research
- Plant Virus Research Studies
- Herpesvirus Infections and Treatments
- Escherichia coli research studies
- Berberine and alkaloids research
- Plant Pathogenic Bacteria Studies
- Toxin Mechanisms and Immunotoxins
- Animal Disease Management and Epidemiology
- T-cell and Retrovirus Studies
- High-Voltage Power Transmission Systems
- Energy Load and Power Forecasting
- Wnt/β-catenin signaling in development and cancer
- Signaling Pathways in Disease
- Monoclonal and Polyclonal Antibodies Research
- Autophagy in Disease and Therapy
- Medical Research and Treatments
Ministry of Agriculture and Rural Affairs
2022-2024
Yangzhou University
2010-2024
Jiangsu Academy of Agricultural Sciences
2014-2024
State Key Laboratory of Food Science and Technology
2022
Jilin Academy of Agricultural Sciences
2021
Xiaomi (China)
2010-2013
Nanjing Agricultural University
2011-2012
Ministry of Agriculture
2011
China Agricultural University
2005-2010
Theodore Roosevelt High School
1989-1990
In previous studies in intact 3T3-Ll fibroblasts and adipocytes, we demonstrated that the phosphorylation state of an acidic, multicomponent M, 80,000 protein appeared to be a specific useful marker for activation kinase C (Blackshear, P.
This study aimed to investigate the polymorphism of porcine-origin Ral guanine nucleotide dissociation stimulator (RalGDS) in LLC-PK1 cells using RT-PCR and sequencing. Our results revealed seven polymorphisms RalGDS gene, including insertions, deletions, frameshift mutations. These variations may significantly alter protein structure function RalGDS, potentially influencing its role GTPase-mediated signaling pathways. work provides foundational insights into genetic diversity porcine...
We report here the genome sequence of a porcine circovirus-like agent. The sequenced this agent is composed 648-nucleotide circular DNA that includes three predicted protein-coding genes, which means should be novel member family Circoviridae.
ABSTRACT Porcine bocavirus 5 is a novel porcine species found in pig with clinical diarrhea from farm China. Here, we report the complete genome sequence of strain PBoV5/JS677, which will help toward understanding molecular and evolutionary characteristics bocavirus.
Streptococcus suis serotype 2 (SS2) is an important swine and human pathogen responsible for septicemia meningitis. The bacterial homologues of eukaryotic-type serine/threonine kinases (ESTKs) have been reported to play critical roles in various cellular processes. To investigate the role STK SS2, isogenic stk mutant strain (Δstk) a complemented (CΔstk) were constructed. Δstk showed significant decrease adherence HEp-2 cells, compared with wild-type strain, reduced survival ratio whole...
A novel porcine pathogen tentatively named P1, which was obtained from the sera of pigs exhibiting clinical signs postweaning multisystemic wasting syndrome (PMWS) experimentally caused classical clinic and pathologic lesions disease in by direct vivo injection with P1 DNA plasmids. Twenty colostrum-fed (CF) that were free PCV2 at 1 month age randomly designated equally to two groups. Group each injected 400 µg cloned plasmid into superficial inguinal lymph nodes 2 same amount empty pSK...
Abstract The recently discovered porcine boca-like virus (Pbo-likeV) is a member of the Parvoviridae family, genus Bocavirus , and it potentially associated with swine disease. Several studies have Pbo-likeV postweaning multisystemic wasting syndrome in pigs, but full spectrum clinical disease epidemiology infection remain unclear. availability rapid reliable molecular diagnostics would aid future this novel virus. Thus, we developed sensitive specific TaqMan-based real-time PCR assay to...
Abstract Recently, we identified a novel porcine circovirus type 2-like agent P1 isolate from swine. The present study represents the first survey of prevalence in swine herds Jiangsu, China, by using PCR targeting complete genome P1. Prevalences 50% and 19% were found among 6 248 animals, respectively. results indicate high China pig populations.
A field porcine epidemic diarrhea virus (PEDV) strain, JS2008, was isolated from stool samples of a piglet with acute on vaccinated farm in eastern China. We sequenced and analyzed the complete genome strain which will help increase our understanding molecular characteristics PEDV
We first report here the genome sequences of 4 rearranged porcine circovirus type 2 strains, JSTZ, ZJQDH1, ZJQDH2, and JSHM, isolated from sera in China. The complete circular genomes these isolates are 578, 483, 574, 772 nucleotides length, respectively. They predicted to be defective interfering particles 2. findings will help us understand molecular evolution relationship between diseases.
Outbreaks of trembling and shaking were reported among pigs at two pig farms in Jiangsu Province, China. Serum tissue samples tested positive for porcine circovirus-like virus P1 negative classical swine fever virus, circovirus type 2, astrovirus pestivirus using PCR/RT-PCR immunohistochemical techniques. High viral genome loads identified sera, brain lymph node by qPCR. In addition, one the most notable pathological changes was dissolution nucleus Purkinje cells. The results this study...
Nitric oxide (NO), an important signaling molecule with biological functions, has antimicrobial activity against a variety of pathogens including viruses. To our knowledge, little information is available about the regulatory effect NO on porcine circovirus type 2 (PCV2) infection. This study was conducted to investigate antiviral generated from S-nitrosoglutathione (GSNO), during PCV2 infection PK-15 cells and BALB/c mice.GSNO released considerable in culture medium cells, scavenged by its...
Recently identified porcine circovirus-like virus P1 has the smallest DNA viral genome. In this study, we genes and their corresponding mRNA transcripts. The RNAs of P1, synthesized in kidney cells, were examined with northern blotting PCR analyses. Eight virus-specific detected. Four mRNAs (open reading frames (ORFs) 1, 2, 4, 5) are encoded by (−) strand four (ORFs 3, 6, 7, 8) (+) strand. All proteins ORFs less than 50 amino acids length, except that ORF1 (113 acids). We show a very complex...
Porcine circovirus-like virus P1, a novel single-stranded circular DNA virus, is potential causative agent of post-weaning multisystemic wasting syndrome in swine. In this study, we detected P1 strains serum samples from cattle, goats and rabbits suffering different clinical symptoms, Jiangsu Province, China. We sequenced the whole genomes six strains, which showed 99.4%–100% nucleotide sequence similarity were closely related to other porcine-origin isolates. The sequences...
ABSTRACT Partetravirus is a novel defined genus of animal parvoviruses. Here, we first report the genome sequence porcine partetravirus strain JSNJ62, which highly prevalent in mainland China. It will help understanding epidemiology and molecular characteristics partetravirus.
Porcine circovirus type 2 (PCV2) is the etiologic agent of porcine circovirus-associated disease. Here, we first report complete genome sequence PCV2 strain JSTZ, which was isolated from piglet stool samples and highly prevalent in China. It will help understanding epidemiology molecular characteristics PCV2.