A5068069651- Reproductive tract infections research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Gut microbiota and health
- Long-Term Effects of COVID-19
- Urinary Tract Infections Management
- Adolescent Sexual and Reproductive Health
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Probiotics and Fermented Foods
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Glutathione Transferases and Polymorphisms
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Genomics, phytochemicals, and oxidative stress
- Histone Deacetylase Inhibitors Research
- Respiratory viral infections research
- Pelvic floor disorders treatments
- Cancer-related cognitive impairment studies
- Protein Degradation and Inhibitors
- CAR-T cell therapy research
- HIV/AIDS Research and Interventions
- Frailty in Older Adults
- Childhood Cancer Survivors' Quality of Life
University of Michigan
2020-2025
Michigan United
2020-2025
Stony Brook University
2024
Stony Brook University Hospital
2024
Memorial Sloan Kettering Cancer Center
2021
University of Virginia
2018-2020
Pennsylvania State University
2010
Abstract The hallmarks of COVID-19 are higher pathogenicity and mortality in the elderly compared to children. Examining baseline SARS-CoV-2 cross-reactive immunological responses, induced by circulating human coronaviruses (hCoVs), is needed understand such divergent clinical outcomes. Here we show analysis coronavirus antibody responses pre-pandemic healthy children ( n = 89), adults 98), 57), patients 50) systems serology. Moderate levels cross-reactive, but non-neutralizing, antibodies...
Compared to adults, children with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have predominantly mild or asymptomatic infections, but the underlying immunological differences remain unclear. Here, we describe clinical features, virology, longitudinal cellular, and cytokine immune profile, SARS-CoV-2-specific serology salivary antibody responses in a family of two parents PCR-confirmed symptomatic SARS-CoV-2 infection their three children, who tested repeatedly PCR negative....
Respiratory tract infection with SARS-CoV-2 results in varying immunopathology underlying COVID-19. We examine cellular, humoral and cytokine responses covering 382 immune components longitudinal blood respiratory samples from hospitalized COVID-19 patients. SARS-CoV-2-specific IgM, IgG, IgA are detected blood, however, receptor-binding domain (RBD)-specific IgM IgG seroconversion is enhanced specimens. neutralization activity correlates RBD-specific levels. Cytokines/chemokines vary between...
The availability of high-affinity agonists for peroxisome proliferator-activated receptor-β/δ (PPARβ/δ) has led to significant advances in our understanding the functional role PPARβ/δ. In this study, a new PPARβ/δ antagonist, 4-chloro-<i>N</i>-(2-{[5-trifluoromethyl)-2-pyridyl]sulfonyl}ethyl)benzamide (GSK3787), was characterized using vivo and vitro models. Orally administered GSK3787 caused antagonism...
Abstract SARS-CoV-2, the pandemic coronavirus that causes COVID-19, has infected millions worldwide, causing unparalleled social and economic disruptions. COVID-19 results in higher pathogenicity mortality elderly compared to children. Examining baseline SARS-CoV-2 cross-reactive immunological responses, induced by circulating human coronaviruses, is critical understand such divergent clinical outcomes. The cross-reactivity of antibody responses healthy children (n=89), adults (n=98),...
Abstract Bacterial vaginosis is a condition associated with adverse reproductive outcomes and characterized by shift from Lactobacillus -dominant vaginal microbiota to polymicrobial microbiota, consistently colonized strains of Gardnerella vaginalis . Metronidazole the first-line treatment; however, treatment failure recurrence rates remain high. To understand complex interactions between involved in efficacy, here we develop an ordinary differential equation model that predicts bacterial...
An NRF2-p53 oxidative stress response network may promote both normal breast development and tumor progression.
Bacterial vaginosis ( BV ) is a common condition characterized by shift in vaginal microbiome composition that linked to negative reproductive outcomes and increased susceptibility sexually transmitted infections. Despite the commonality of BV, standard-of-care antibiotics provide limited control recurrent episodes development new biotherapies lack controlled models needed evaluate dosing treatment regimens. Here, we develop an silico framework selection criteria for potential probiotic...
Despite the efficacy of antiretroviral-based pre-exposure prophylactics (PrEP) in men who have sex with men, studies women produced widely varying outcomes. Recent evidence demonstrates that vaginal microbial communities are associated increased HIV acquisition risk and may impact PrEP efficacy. Here, we investigate mechanisms underlying how bacteria alter drug levels infection rates ex vivo . Using cervicovaginal lavages (CVLs) from or without bacterial vaginosis (BV), identified metabolism...
Immunoglobulin G (IgG) antibodies that activate Fc-mediated immune functions have been correlated with vaccine efficacy, but it is difficult to unravel the relative roles of multiple IgG and Fc receptor (FcR) features capacity influence IgG-FcR complex formation vary on a personalized basis. Here, we develop an ordinary differential-equation model determine how variability in subclass concentrations binding affinities IgG-FcγRIIIa validate samples from HIV RV144 trial. The identifies...
The vaginal microbiome (VMB) is a complex microbial community that closely tied to reproductive health. Optimal VMB communities have compositions are commonly defined by the dominance of certain Lactobacillus spp. and can remain stable over time or transition non-optimal states dominated anaerobic bacteria associated with bacterial vaginosis (BV). ability undergo transitions suggests system either single (mono-stable) multiple (multi-stable) equilibrium states, though factors contribute...
Fc-mediated immune functions have been correlated with protection in the RV144 HIV vaccine trial and are important for immunity to a range of pathogens. IgG antibodies (Abs) that form complexes Fc receptors (FcRs) on innate cells can activate functions. Genetic variation both IgGs FcRs capacity alter IgG-FcR complex formation via changes binding affinity concentration. A growing challenge lies unraveling importance multiple variations, especially context trials conducted homogenous genetic...
Abstract Bacterial vaginosis is a common condition characterized by shift in vaginal microbiome composition that linked to negative reproductive outcomes and increased susceptibility sexually transmitted infections. Despite the commonality of BV, standard-of-care antibiotics provide limited control recurrent BV episodes development new biotherapies lack controlled models needed evaluate dosing treatment regimens. Here, we develop an silico framework selection criteria for potential probiotic...
e23212 Background: Treatment of oncologic disease with Bispecific T-Cell Engager antibodies (BiTEs) are an emerging novel immunotherapy demonstrated efficacy in multiple hematologic malignancies. Notable side effects BiTE therapy include cytokine release syndrome (CRS) and immune effector cell-associated neurotoxicity (ICANS), which can be life-threatening must rapidly identified. We aim to fill knowledge gaps surrounding CRS ICANS for medical providers, particularly considering increased...
ABSTRACT Although the respiratory tract is primary site of SARS-CoV-2 infection and ensuing immunopathology, immune responses are understudied urgently needed to understand mechanisms underlying COVID-19 disease pathogenesis. We collected paired longitudinal blood samples (endotracheal aspirate, sputum or pleural fluid) from hospitalized patients non-COVID-19 controls. Cellular, humoral cytokine were analysed correlated with clinical data. SARS-CoV-2-specific IgM, IgG IgA antibodies detected...
Although the respiratory tract is primary site of SARS-CoV-2 infection and ensuing immunopathology, immune responses are understudied urgently needed to understand mechanisms underlying COVID-19 disease pathogenesis. We collected paired longitudinal blood samples (endotracheal aspirate, sputum or pleural fluid) from hospitalized patients non-COVID-19 controls. Cellular, humoral cytokine were analysed correlated with clinical data. SARS-CoV-2-specific IgM, IgG IgA antibodies detected using...
Sickle Cell Anemia is an inherited recessive disorder caused by a single point mutation in the human beta globin (HBB) gene resulting abnormal type of hemoglobin. Here we report targeting HBB locus using CRISPR/Cas9 technology for correction Disease, which affects nearly 1 million people worldwide. Cas9 and its variants can be used to introduce variety breaks including blunt double stranded break (DSB), nicks, or dual nicks leaving either 3' 5' overhang. The cut donor play role triggering...
The phosphorylation of histone H3 threonine 45 (H3T45ph) in S. cerevisiae and mammalian cells occurs at the N‐terminal alpha‐helix aligns precise location DNA points entry exit on nucleosome. peaks during S‐phase is mediated by replication kinase Cdc7, which dependent forming a complex with Dbf4 to phosphorylate residue. Loss T45ph leads greater sensitivity replicative stress, but it not required for cell survival. ChIP‐seq was used identify genomic sites H3T45ph asynchronous hydroxyurea...