A5085060592- Hearing, Cochlea, Tinnitus, Genetics
- Hearing Loss and Rehabilitation
- Vestibular and auditory disorders
- Ear and Head Tumors
- Ear Surgery and Otitis Media
- Meningioma and schwannoma management
- Neurofibromatosis and Schwannoma Cases
- Music and Audio Processing
- Speech and Audio Processing
- Nasal Surgery and Airway Studies
- RNA regulation and disease
- Allergic Rhinitis and Sensitization
- Sinusitis and nasal conditions
- RNA Interference and Gene Delivery
- Brain Metastases and Treatment
- Asthma and respiratory diseases
- Head and Neck Surgical Oncology
- Extracellular vesicles in disease
- Facial Nerve Paralysis Treatment and Research
- Silk-based biomaterials and applications
- CRISPR and Genetic Engineering
- Glioma Diagnosis and Treatment
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Ultrasound Imaging and Elastography
Vienna General Hospital
2016-2024
Medical University of Vienna
2014-2024
Stanford University
2024
Universitätsklinik für Hals-, Nasen- und Ohrenheilkunde
2024
Christian Doppler Laboratory for Thermoelectricity
2023-2024
University of Vienna
2024
Massachusetts Eye and Ear Infirmary
2014-2023
Harvard University
2014-2023
Eaton (United States)
2018-2022
Universidade de Brasília
2016
Abstract Vestibular schwannomas (VSs) are the most common tumours of cerebellopontine angle. Ninety-five percent people with VS present sensorineural hearing loss (SNHL); mechanism this SNHL is currently unknown. To establish first model to study role VS-secreted factors in causing SNHL, murine cochlear explant cultures were treated human tumour secretions from thirteen different unilateral, sporadic VSs subjects demonstrating varied degrees ipsilateral SNHL. The extent damage due secretion...
Objectives/Hypothesis Only a few reports describe the outcomes following endoscopic ear surgery (EES) in children for chronic disease. We differentiate between transcanal (TEES), where case is performed with only visualization, from non‐TEES, endoscope not used at all or as an adjunct to microscope. hypothesize that EES effective approach manage middle pathology using most cases, and can be incorporated into pediatric otology practice neutral positive effect on outcomes. Lessons learned...
Abstract Inner ear gene therapy using adeno-associated viral vectors (AAV) promises to alleviate hearing and balance disorders. We previously established the benefits of Anc80L65 in targeting inner outer hair cells newborn mice. To accelerate translation humans, we now report feasibility efficiency surgical approach vector delivery a nonhuman primate model. Five rhesus macaques were injected with AAV1 or expressing eGFP transmastoid posterior tympanotomy access round window membrane after...
Objective Periostin is an extracellular matrix protein that elevated in the sinonasal tissues of patients with chronic rhinosinusitis (CRS). The purpose this study was to determine whether serum periostin could serve as a molecular biomarker nasal polyp burden disease. Study Design Prospective cohort study. Setting Academic medical center. Subjects and Methods Serum levels were measured by ELISA on blood samples collected from undergoing sinus surgery for CRS (n = 71), further stratified...
Optogenetics is a transformative technology based on light-sensitive microbial proteins, known as opsins, that enable precise modulation of neuronal activity with pulsed radiant energy. has been proposed means to improve auditory implant outcomes by reducing channel interaction and increasing electrode density, but the introduction opsins into cochlear spiral ganglion neurons (SGNs) in vivo challenging. Here we test opsin delivery using synthetically developed ancestral adeno-associated...
Vestibular schwannoma (VS) is a tumor of the vestibular nerve that transmits balance information from inner ear to brain. Sensorineural hearing loss occurs in 95% patients with these tumors, but cause this not well understood. We posit role VS-secreted extracellular vesicles (EVs) as major contributing factor cochlear damage. Using differential centrifugation, we isolated EVs VS cell line HEI-193 and primary cultured human cells good or poor hearing. The were characterized using Nanosight...
Normalizing the tumor microenvironment with losartan prevents tumor-induced hearing loss and enhances radiation response in schwannoma rodent models.
Hearing impairment is the most common sensory disorder in humans, and yet hardly any medications are licensed for treatment of inner ear pathologies. Intricate pharmacokinetic examinations to better understand drug distribution within this complex organ could facilitate development novel therapeutics. For such translational research projects, animal models indispensable, but differences dimensions other anatomical features complicate transfer experimental results clinic. The gap between...
Abstract Our sense of hearing is mediated by cochlear hair cells, which there are two types organized in one row inner cells and three rows outer cells. Each cochlea contains 5–15 thousand terminally differentiated their survival essential for as they do not regenerate after insult. It often desirable research to quantify the number within samples, both pathological conditions, response treatment. Machine learning can be used automate quantification process but requires a vast diverse...
Alzheimer's disease (AD) accounts for more than 60% of the dementia cases and currently there is no curative treatment it. With emergence potentially modifying treatments, early diagnosis key to identify patient groups that would benefit from such aiming prevent severe cognitive decline. We previously identified a set DNA methylation signatures allow accurate AD in cortical neurons brain tissue, even before clinical manifestation [1]. Here we investigate 11 these signature regions via...
Sensorineural hearing loss (SNHL) is the most common sensory deficit worldwide, frequently caused by noise trauma and aging, with inflammation being implicated in both pathologies. Here, we provide first direct measurements of proinflammatory cytokines inner ear fluid, perilymph, adolescent 2-year-old mice. The perilymph mice exposed to intensity resulting permanent auditory threshold elevations had significantly increased levels IL-6, TNF-α, CXCL1 6 h after exposure, elevated (19.3 ± 6.2...
Significance In patients with progressive vestibular schwannoma (VS), radiotherapy is associated the risk of debilitating hearing loss. There an urgent need to identify adjunct therapy that, by enhancing efficacy radiation, can help lower radiation dose and preserve hearing. our newly developed cerebellopontine angle model schwannomas that faithfully recapitulates tumor-induced loss, we demonstrate cMET blockade sensitizes (RT) in neurofibromatosis type II animal models without any adverse...
It has been shown that glucocorticoids reduce the hearing threshold shifts associated with cochlear implantation. Previous studies evaluated administration of immediately before surgery or repeated pre- perioperative systemic application glucocorticoids. The aim this study was to evaluate effects a sustained release dexamethasone hydrogel in preservation To address issue, guinea pig model implantation used. 30 normal pigmented pigs were randomized into group receiving single dose...
Chronic rhinosinusitis (CRS) is a common disease that substantially impairs the quality of life (QoL). Here, we aimed to assess patients’ QoL in different subtypes CRS and correlated this with nasal polyp size improve clinical understanding burden disease. In retrospective single-center study, 107 patients following diagnoses were analyzed: without polyps (CRSsNP), (CRSwNP), or aspirin-exacerbated respiratory (AERD). Sino-Nasal Outcome Test-20 German Adapted Version (SNOT-20 GAV) scores...
Objective: The aim of the study was to evaluate predictive value regarding postoperative hearing benefit electrically evoked auditory brainstem response audiometry in sporadic vestibular schwannoma patients undergoing simultaneous tumor resection and cochlear implantation. Design: Patients were included a prospective conducted between October 2016 January 2019. Setting: at tertiary care center. Participants: Subjects with unilateral screened for participation. underwent translabyrinthine...
Nonsteroidal anti-inflammatory drug (NSAID)-exacerbated respiratory disease (N-ERD) comprises the triad of chronic rhinosinusitis with nasal polyps, asthma and intolerance to NSAIDs. Dupilumab treatment, targeting interleukin-4 (IL-4) receptor α, significantly reduces polyp burden as well symptoms. Here we aimed investigate effect dupilumab on aspirin intolerance, cytokine profiles in patients N-ERD.In this open-label trial, adult confirmed N-ERD were treated for 6 months. Clinical...
Vestibular schwannoma (VS) is an intracranial tumor arising from neoplastic Schwann cells and typically presenting with hearing loss. The traditional belief that deficit caused by physical expansion of the VS, compressing auditory nerve, does not explain common clinical finding patients small tumors can have profound loss, suggesting tumor-secreted factors could influence ability in VS patients. We conducted profiling patients’ plasma for 66 immune-related sporadic ( N > 170) identified...
Vestibular schwannomas (VSs), the most common tumors of cerebellopontine angle, arise from Schwann cells lining vestibular nerve. Pharmacotherapies against VS are almost non‐existent. Although therapeutic inhibition inflammatory modulators has been established for other neoplasms, it not explored in VS. A bioinformatic network analysis all genes reported to be differentially expressed human revealed a pro‐inflammatory transcription factor nuclear factor‐kappa B (NF‐κB) as central molecule...
Tumor necrosis factor-alpha (TNF-alpha) is a proinflammatory cytokine that plays prominent role in the nervous system, mediating range of physiologic and pathologic functions. In auditory elevated levels TNF-alpha have been implicated several types sensorineural hearing loss, including loss induced by vestibular schwannoma, potentially fatal intracranial tumor originates from eighth cranial nerve; however, mechanisms underlying tumor's deleterious effects on are not well understood. Here, we...