A5082981151- Lymphatic System and Diseases
- Immune Cell Function and Interaction
- Sphingolipid Metabolism and Signaling
- Lymphatic Disorders and Treatments
- Cerebrospinal fluid and hydrocephalus
- T-cell and B-cell Immunology
- Ion Transport and Channel Regulation
- Inhalation and Respiratory Drug Delivery
- Proteoglycans and glycosaminoglycans research
- Immunotherapy and Immune Responses
- IL-33, ST2, and ILC Pathways
- Pancreatitis Pathology and Treatment
- Advancements in Transdermal Drug Delivery
- Glycosylation and Glycoproteins Research
- Neonatal Respiratory Health Research
- Myofascial pain diagnosis and treatment
- Renal Transplantation Outcomes and Treatments
- CAR-T cell therapy research
- Diet and metabolism studies
- Cancer Immunotherapy and Biomarkers
- Advanced Neuroimaging Techniques and Applications
- Immunodeficiency and Autoimmune Disorders
- Drug Solubulity and Delivery Systems
- Cholesterol and Lipid Metabolism
- Lipid Membrane Structure and Behavior
Monash University
2013-2024
Drugs are commonly administered via the intraperitoneal (IP) route to treat localized infections and cancers in patients test drug efficacy toxicity preclinical studies. Despite this, there remain large gaps our understanding of absorption routes (lymph vs blood) pharmacokinetics following IP administration. This is particularly true when drugs complex delivery systems such as liposomes which main marketed formulation for several that intraperitoneally. study investigated impact liposome...
The intestinal lymphatic system transports fluid, immune cells, dietary lipids, and highly lipophilic drugs from the intestine to systemic circulation. These transport functions are important health when dysregulated contribute pathology. This has generated significant interest in approaches deliver lymphatics. Most of current understanding lymph flow, lipid drug rates, comes vitro studies vivo animal studies. In contrast, human subjects have been limited. Recently, three surgical patients...
Recently, peripheral lymphatic vessels were found to transport high-density lipoprotein (HDL) from interstitial tissues the blood circulation during reverse cholesterol transport. This function is thought be critical clearance of atherosclerotic plaques. The role organ-specific lymphatics in modulating HDL and composition is, however, incompletely understood. study aimed 1) determine contribution draining intestine liver (which are major sites synthesis) total (thoracic) lymph 2) verify...
Interstitial administration (e.g., subcutaneous (SC) administration) of immunotherapies and vaccines within nanoparticles can improve access to lymph-resident immune cells, leading enhanced efficacy reduced off-target effects. Recently, endogenous high-density lipoproteins (HDLs) were found return from peripheral tissue back the systemic circulation via lymphatic vessels nodes. This suggests potential utility HDLs as biocompatible lymphatic-targeted therapeutic carriers. However, we have a...
The mesenteric lymph nodes (MLN) are a key site for the generation of adaptive immune responses to gut-derived antigenic material and cells within MLN contribute pathophysiology range conditions including inflammatory autoimmune diseases, viral infections, graft versus host disease cancer. Targeting immunomodulating drugs may thus be beneficial in conditions. This paper investigates potential benefit targeting model immunosuppressant drug, mycophenolic acid (MPA), T MLN, using triglyceride...
Background: Fluids, solutes and immune cells have been demonstrated to drain from the brain surrounding structures cervical lymph vessels nodes in neck via meningeal lymphatics, nasal lymphatics and/or lymphatic associated with cranial nerves. A method cannulate efferent duct for continuous fluid collection rodents has not described previously would assist evaluating transport of molecules head as well changes composition different physiological challenges or diseases. Aim: To develop a...
In recent years, the drainage of fluids, immune cells, antigens, fluorescent tracers, and other solutes from brain has been demonstrated to occur along lymphatic outflow pathways deep cervical lymph nodes in neck. To best our knowledge, no studies have evaluated transport therapeutics brain. The objective this study was determine model different molecular weights lipophilicity using cannulation ligation models rats. do this, anesthetized Sprague–Dawley rats were cannulated at carotid artery...
Abstract Objective Conventionally, in vivo mesenteric lymphatic contractile function is measured using a high magnification transmission microscope (field of view 0.3–1.5 mm), which precludes visualization extended lengths vessels embedded fat. Existing software not optimized for imaging at low contrast agent. We aimed to develop simple and clinically transferable method situ visualization, image analysis, quantitative assessment over an area. Methods Subserosal injection various blue dyes...
Changes to the number, type, and function of immune cells within joint-draining lymphatics is a major contributor progression inflammatory arthritis. In particular, there significant expansion in pathogenic B lymph node (jdLN). These appear clog lymphatic sinuses node, inhibit flow, therefore, reduce clearance fluid from joint. Taken together, potential treat arthritis more effectively, as well off-target side effects, with localized delivery B-cell depleting therapies jdLNs. We recently...
Consumption of a diet rich in saturated fat increases lipid absorption from the intestine, assembly into chylomicrons, and delivery to metabolic tissues via lymphatic circulatory systems. Accumulation ceramide lipids, composed sphingosine fatty acid, contributes pathogenesis cardiovascular diseases, type 2 diabetes mellitus cancer. Using mesenteric lymph duct cannulated rat model, we showed that ceramides are generated by intestine assembled which transported system. A lipidomic screen...
Abstract The tumor-draining lymph node (TDLN) modulates anti-tumor immunity. Targeting immune checkpoint blockade monoclonal antibodies (mAbs) to the TDLN via subcutaneous (SC) delivery has potential enhance efficacy and safety. Recombinant human hyaluronidase (rHuPH20; PH20) transiently removes hyaluronan at interstitial injection sites is used facilitate SC of large doses volumes required for mAbs. We have previously shown that PH20 co-dosing improves rate extent lymphatic absorption Here...