A5070630878- Enzyme Catalysis and Immobilization
- Catalysis for Biomass Conversion
- Asymmetric Hydrogenation and Catalysis
- Innovative Microfluidic and Catalytic Techniques Innovation
- Mesoporous Materials and Catalysis
- Chemical Reactions and Isotopes
- Microbial Metabolic Engineering and Bioproduction
- Synthesis and Catalytic Reactions
- Nanomaterials for catalytic reactions
- Carcinogens and Genotoxicity Assessment
- Chemical Synthesis and Analysis
- Toxic Organic Pollutants Impact
- Electrochemical sensors and biosensors
- Amino Acid Enzymes and Metabolism
- Effects and risks of endocrine disrupting chemicals
- Cyclopropane Reaction Mechanisms
- Protein Kinase Regulation and GTPase Signaling
- Herbal Medicine Research Studies
- Ubiquitin and proteasome pathways
- Supramolecular Chemistry and Complexes
- Biofuel production and bioconversion
- Respiratory and Cough-Related Research
- Click Chemistry and Applications
- Drug-Induced Hepatotoxicity and Protection
- Metabolomics and Mass Spectrometry Studies
Hebei University of Technology
2016-2025
Incyte (United States)
2024-2025
University of Arizona
2011-2013
Shenyang Pharmaceutical University
2004
Abstract The direct asymmetric reductive amination of heteroaryl ketones has been a long‐standing synthetic challenge. Here we report the engineering an amine dehydrogenase (AmDH) from Jeotgalicoccus aerolatus for synthesis chiral α‐(hetero)aryl primary amines in excellent conversions (up to 99 %) and enantioselectivities % ee). best AmDH variant ( Ja ‐AmDH‐M3 3 ) exhibited high activity specificity toward alkyl (hetero)aryl ketones, even those bearing bulky chain. An efficient directed...
For synthesizing glycerol carbonate (GC) by a reaction between (GL) and dimethyl (DMC), lipase immobilized on magnetic organosilica nanoflowers was prepared utilized as biocatalyst. Candida antarctica B (CALB) chosen model enzyme for preparing an biocatalyst (CALB@nanoflowers). The obtained CALB@nanoflowers characterized using scanning electron microscopy, transmission confocal laser microscopy. Effects of GL/DMC molar ratio, amount, temperature, surfactant molecular sieve addition, time the...
The highly efficient chemoselectivity, stereoselectivity, and regioselectivity render enzyme catalysis an ideal pathway for the synthesis of various chemicals in broad applications. While cofactor is necessary but expensive, conversed state not beneficial positive direction reaction. Cofactor regeneration using electrochemical methods has advantages simple operation, low cost, easy process monitoring, product separation, electrical energy green sustainable. Therefore, bioelectrocatalysis...
The inhibition of mutant KRAS proteins has emerged as a promising approach for treating KRAS-driven cancers, evidenced by the clinical success G12C inhibitors. G12D, most common mutant, promises significant expansion addressable patient population; however, reduced nucleophilicity aspartate compared to cysteine poses challenges in balancing sufficient potency with ADME properties support oral exposure. Herein, we describe discovery G12D inhibitor 23 (INCB159020), which achieves exposure...
<title>Abstract</title> While microbial cells have emerged as a versatile platform for the synthesis of metal nanoparticles, their application production single-atom catalysts (SACs) has been rarely studied yet. Here, we develop facile method ambient SACs with high loading >4.0 wt% by in-situ reduction ions on overexpressing catalytically active enzyme (e.g., alcohol dehydrogenase (ADH), amine and monoamine oxidase), producing chemo-bio bifunctional (SAC@cell). The ADH-overexpressed...
Dendritic mesoporous silica nanoparticles (DMSNs) are a new class of solid porous materials used for enzyme immobilization support due to their intrinsic characteristics, including unique open central–radial structures with large pore channels and excellent biocompatibility. In this review, we review the recent progress in research on using DMSNs different structures, namely, flower-like tree-branch-like DMSNs. Three DMSN synthesis methods briefly compared, distinct characteristics two types...
Ketoreductases(KREDs), which play an important role in the preparation of chiral alcohol drug intermediates, are promising biocatalysts that can lead to become a greener and more sustainable process. Two decades ago, KREDs were widely researched from protein structure catalytic mechanism, many researchers worked on properties research. And with engineering development, useful production This review has summarized applications, perspective biocatalysis, providing detailed introduction...
Abstract KRAS G12D (G12D) is one of the most frequent oncogenic driver mutations, and especially common in pancreatic (PDAC) colorectal (CRC) cancers. Patients with G12D-mutated disease experience poor treatment outcomes, representing a significant unmet medical need. mutation results constitutively active signaling, including hyperactivation ERK PI3K pathways, which drive cell proliferation survival. Here we describe INCB161734, novel, potent, selective, orally bioavailable small molecule...
The polycyclic aromatic hydrocarbon pollutant benzo[a]pyrene (BaP) is a known developmental gonadotoxicant. However, the mechanism of BaP-induced germ cell death unclear. We investigated whether exposure to BaP induces apoptotic in mouse fetal ovary or testis. Mouse gonads were dissected at embryonic day 13.5 days postcoitum (dpc) and fixed immediately cultured for 6, 24, 48, 72 h with various concentrations (1–1000 ng/ml). Germ cells numbers, apoptosis, proliferation evaluated by...
The enantioselective synthesis of chiral fluorinated amines is great importance but highly challenging in synthetic chemistry and the pharmaceutical industry. Herein, we established a chemoenzymatic cascade for α-mono- difluoromethyl from easily available β-keto-acid esters water–oil–solid multiphasic systems via two catalytic modules, i.e., organo-enzymatic decarboxylative fluorination bienzymatic reductive amination. An efficient continuous system was constructed by stepwise enzyme...
Multienzyme cascades (MECs) have gained much attention in synthetic chemistry but remain far from being a reliable tool. Here we report four-enzyme cascade comprising cofactor-independent and cofactor self-sustaining bienzymatic modules for the enantioselective benzylic C-H amination of arylalkanes, challenging transformation bulk chemicals to high value-added chiral amines. The two were subsequently optimized by enzyme co-immobilization with microenvironmental tuning, finally integrated...
Core-shell structured magnetic wrinkled organosilica-based metal-enzyme integrated catalysts were synthesized, and their catalytic performances studied in the chemoenzymatic dynamic kinetic resolution of chiral amines an organic solvent, as well synthesis alcohols water. Structure-performance studies revealed important influence tunable structure composition on optimization activity, stability, recyclability catalysis.
Abstract Nature has evolved highly efficient and complex systems to perform cascade reactions by the elegant combination of desired enzymes, offering a strategy for achieving bioprocess intensification. Chemoenzymatic (CECRs) merge complementary strengths chemo-catalysis bio-catalysis, such as wide reactivity chemo-catalysts exquisite selective properties biocatalysts, representing an important step toward emulating nature construct artificial However, incompatibilities between two catalytic...