Phytochemical investigation of the methanol extract aerial parts Salvia plebeia aided by a proprotein convertase subtilisin/kexin type 9 (PCSK9) mRNA expression screening assay in HepG2 cells led to identification 19 compounds including one new norsesquiterpene (1), six eudesmane sesquiterpenoids (2–5, 8, and 11), 12 known compounds. The structures all were elucidated interpretation their 1D 2D NMR spectroscopic MS data. Furthermore, computational prediction ECD or chemical shifts was used...

Thirteen C-methylated flavonoid glycosides (1–13), along with 15 previously known flavonoids (14–28), were isolated from rhizomes of Pentarhizidium orientale. Among these compounds, matteuorienates D–K (1–8) obtained as analogues A–C (14–16), which contain a characteristic 3-hydroxy-3-methylglutaryl (HMG) moiety. The structures 1–13 characterized by spectroscopic analysis and chemical derivatization. isolates evaluated for their antiviral activities against influenza virus (H1N1), compounds...

In recent years, investigations into the biochemistry of insect-associated bacteria have increased. When combined with analytical dereplication processes, these studies provide a powerful strategy to identify structurally and/or biologically novel compounds. Non-ribosomally synthesized cyclic peptides broad bioactivity spectrum high medicinal potential. Here, we report discovery three new tripeptides: natalenamides A–C (compounds 1–3). These compounds were identified from culture broth...

Porcine epidemic diarrhea virus (PEDV), a serious swine epidemic, has been rampant in Asia since the 1990s. Despite widespread use of PEDV vaccines, occurrence variants requires discovery new substances that inhibit these viruses. During search for inhibitory materials from natural sources, seven sabphenosides (1–7) and flavonoid (8), as well eight known phenolic compounds (9–16), were obtained leaves Sabia limoniacea. The structural determination derivatives (1–8) was accomplished using...

Abstract Commercially cultivated Limnospira (species formerly classified to genus Arthrospira ) is a popular food/supplement consumed by millions of people worldwide for health benefits. The objective the current research was advance standardization technology . Quantitative methods were established detect fatty acids as potential chemical markers and immune-enhancing activity. Analysis 20 different batches biomass obtained from one commercial grower demonstrated that there statistically...

Six new dimeric lignans (1–6) and one lignan glycoside (16) were isolated from Forsythia viridissima roots along with nine known (7–15). Spectroscopic analyses chemical methods used to determine these structures their absolute configurations. Among compounds, dimatairesinol (1) viridissimaols A–E (2–6) assigned as dimers of dibenzylbutyrolactone analogues. Furthermore, the compounds evaluated for antiviral activities against coxsackievirus B3 (CVB3) human rhinovirus 1B (HRV1B). In tests, 12...

A methanolic extract of the roots Polygala tenuifolia (Polygalaceae) significantly attenuated nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated BV2 microglia cells. Five xanthones, 1-hydroxy-7-methoxyxanthone (1), 3,6-dihydroxy-1,2,7-trimethoxyxanthone (2), 1,3,6-trihydroxy-2,7-dimethoxyxanthone (3), 1,7-dihydroxy-2,3-dimethoxyxanthone (4) and 1,7-dihydroxy-3-methoxyxanthone (5), five phenylpropanoids, 4-hydroxy-3-methoxypropiophenone (6), methyl 4-hydroxy-3-methoxycinnamic...

In this study, the chemical diversity of polyphenols in Iris lactea var. chinensis seeds was identified by combined MS/MS-NMR analysis. Based on annotated profile, isolation stilbene oligomers conducted, and consequently, (1-10) were characterized. Of these, compounds 1 2 are previously undescribed dimer glycoside (1) tetramer (2), respectively. Besides, to evaluate plant seed as a rich source oligomers, we quantified three I. seeds. The contents major oligomers—trans-ε-viniferin (3),...

This study was aimed to evaluate the potential neurotrophic effect of Lycium barbarum fruit extract (LB-E) via increased neurite outgrowth and synaptic formation, its neuroprotective efficacy also checked in vitro. LB-E exerted effects by regulating TrkA/ERK/CREB signaling. In nervous system, promoted neuronal survival differentiation increasing factor secretion C6 glioma cells. Interestingly, expressed levels synaptophysin GAP-43 CNS cell lines. It subsequently number ciliated cells length...

Gaylussacin (1), a stilbene glucoside, has been isolated from Pentarhizidium orientale and is used in Korean folk medicine. Although it was first 1972, the synthesis of gaylussacin never reported. Herein, we report total six steps with an overall yield 23.8%, as well its derivatives. Structurally, contains carboxylic acid glycoside along free phenol on same benzene ring, making selective functionalization for 1 difficult. Heck cross-coupling employed key step to introduce moiety....

Bioassay-guided fractionation of the EtOAc extract from Disporum viridescens roots led to isolation five new benzyl benzoate glycosides, BBGs (1–5). The neuroprotective activities were screened using neuronal HT22 hippocampal cells. BBG-D (4) significantly protected murine cells against glutamate-induced neurotoxicity by maintaining antioxidative defense systems such as superoxide dismutase, glutathione reductase, peroxidase, and content. BBG-D, in a dose-and time-dependent manner, increased...

Lignans are known to be an important class of phenylpropanoid secondary metabolites. In the course our studies on chemodiversity lignans, necessity arose develop a method for fast detection and identification bioactive lignan subclasses. this study, we detected 10 derivatives different extracts F. viridissima by UHPLC-ESI-QTOF-MS. Lignan glycosides (1 2), lignans (3 4), dimers (5-10) were identified analysis their exact masses MSe spectra along with characteristic mass fragmentation patterns...

Cough and phlegm frequently occur in respiratory diseases like upper tract infections, acute bronchitis, chronic obstructive pulmonary diseases. To relieve these symptoms diseases, various ingredients are being used despite the debates on their clinical efficacy. We aimed to investigate effects of extract CKD-497, composed Atractylodis Rhizoma Alba Fructus Schisandrae, relieving cough facilitating expectoration phlegm. CKD-497 was found inhibit inflammatory mediators such as interleukin-8...

Phytochemical investigation on the dried fruits of Casearia grewiifolia led to identification 10 new salicinoyl quinic acid derivatives (1-10), a benzoyl (11), and two known compounds (12 13). The structures were elucidated by interpreting 1D 2D NMR spectroscopic data including HMBC EXSIDE along with chemical method for sugar unit analysis. All isolates evaluated their inhibitory activities against prostaglandin E2 (PGE2) production in ultraviolet B (UVB)-irradiated HaCat keratinocytes. Of...

Liriope platyphylla F.T. Wang & Tang and Ophiopogon japonicus (Thunb.) Ker Gawl are morphologically similar perennial herbaceous plants belonging to the Asparagaceae family used as evergreen ornamental well for medicinal purposes. Complete plastid genomes (plastomes) 45S nuclear ribosomal DNA (45S rDNA) sequences were obtained from two L. accessions one O. using low-coverage, whole-genome data. Phylogenomic analysis plastomes or rDNA of three showed identical topology. Comparative...

Fractionation of a methanol extract Orixa japonica leaves led to the identification five new quinoline alkaloids (1, 2, 4, 8, and 9), three coumarins (15, 17, 19), 20 known compounds. The structures were determined by analysis 1D 2D NMR spectroscopic data. absolute configuration 19 was proposed electronic circular dichroism calculation. Among compounds tested in phenotypic screening measure adiponectin secretion human bone marrow mesenchymal stem cells, metabolites 4 12 stimulated secretions...

Pyrrosia petiolosa (Christ) Ching (Polypodiaceae) has been used for treating gonorrhea, asthma, hematuria, urolithiasis and diuresis in folk medicine. We investigated to isolate the active components from a methanolic extract of this species that showed neuroprotective activities against glutamate-induced oxidative stress HT22 hippocampal cells. The P. was suspended water then fractionated with n-hexane, CHCl3, EtOAc, n-BuOH. Ten compounds were isolated BuOH fraction using various...