A5100698221- Legume Nitrogen Fixing Symbiosis
- Soybean genetics and cultivation
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Plant Micronutrient Interactions and Effects
- Agronomic Practices and Intercropping Systems
- Plant Virus Research Studies
- Plant pathogens and resistance mechanisms
- Nematode management and characterization studies
- CRISPR and Genetic Engineering
- Recycling and Waste Management Techniques
- Public Policy and Administration Research
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Surface Modification and Superhydrophobicity
- RNA Research and Splicing
- Nuclear Structure and Function
- Cassava research and cyanide
- Aluminum toxicity and tolerance in plants and animals
- Neurogenesis and neuroplasticity mechanisms
- Plant Parasitism and Resistance
- Nanocomposite Films for Food Packaging
- Plant Physiology and Cultivation Studies
Guangzhou University
2019-2025
South China Agricultural University
2012-2024
Fujian Agriculture and Forestry University
2009-2024
Nanjing Agricultural University
2012
Soybean (Glycine max) serves as a major source of protein and edible oils worldwide. The genetic genomic bases the adaptation soybean to tropical regions remain largely unclear. Here, we identify novel locus Time Flowering 16 (Tof16), which confers delay flowering improve yield at low latitudes determines that it harbors homolog LATE ELONGATED HYPOCOTYL (LHY). Tof16 previously identified J genetically additively but independently control under short-day conditions. More than 80% accessions...
Photoperiod sensitivity is a key factor in plant adaptation and crop production. In the short-day soybean, to low latitude environments provided by mutations at J locus, which confer extended flowering phase thereby improve yield. The identity of as an ortholog Arabidopsis ELF3, component circadian evening complex (EC), implies that orthologs other EC components may have similar roles. Here we show two soybean homeologs LUX ARRYTHMO interact with form EC. Characterization mutants reveals...
Abstract Legume biological nitrogen (N) fixation is the most important N source in agroecosystems, but it also a process requiring considerable amount of phosphorus (P). Therefore, developing legume varieties with effective N2 under P-limited conditions could have profound significance for improving agricultural sustainability. We show here that inoculation rhizobial strains enhanced soybean (Glycine max) and P nutrition field as well hydroponics. Furthermore, we identified characterized...
Soybean (Glycine max) is an economically important oil and protein crop. Plant height a key trait that significantly impacts the yield of soybean; however, research on molecular mechanisms associated with soybean plant lacking. The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (CRISPR-associated system 9) recently developed technology for gene editing has been utilized to edit genomes crop plants.Here, we designed four gRNAs mutate LATE ELONGATED HYPOCOTYL (LHY)...
Flowering time and plant height are key agronomic traits that directly affect soybean (Glycine max) yield. APETALA1 (AP1) functions as a class A gene in the ABCE model for floral organ development, helping to specify carpel, stamen, petal, sepal identities. There four AP1 homologs soybean, all of which mainly expressed shoot apex. Here, we used clustered regularly interspaced short palindromic repeats (CRISPR) - CRISPR-associated protein 9 technology generate homozygous quadruple mutant,...
The NRAMP (natural resistance-associated macrophage protein) family of genes has been widely characterized in organisms ranging from bacteria to yeast, plants, mice, and humans. This gene plays vital roles divalent metal ion transport across cellular membranes. As yet, comprehensive analysis not reported for soybean. In this study, bioinformatics was conducted identify 13 soybean genes, along with their structures, phylogenetic relationships, transmembrane domains. Expression suggests that...
Rapeseed (Brassica napus) is an important oil crop worldwide. However, severe inhibition of rapeseed production often occurs in the field due to nitrogen (N) deficiency. The root system main organ acquire N for plant growth, but little known about mechanisms underlying adaptions Here, dynamic changes architectural traits N-deficient plants were evaluated by 3D situ quantification. Root proteome responses deficiency analyzed tandem mass tag-based proteomics method, and related proteins...
Symbiotic nitrogen (N2 ) fixation plays a vital role in sustainable agriculture. Efficient N2 requires various materials, including phosphate (Pi); however, the molecular mechanism underlying transport of Pi into nodules and bacteroids remains largely unknown. A nodule-localized transporter, GmPT7, was functionally characterized soybean (Glycine max) its yield investigated via composite whole transgenic plants. GmPT7 protein localized to plasma membrane showed activity for yeast. Altered...
Phosphorus (P) is an essential element for all organisms. Because P fertilizers are a non-renewable resource and high fixation in soils, sustainable agriculture requires researchers to improve crop acquisition efficiency. Here, we report strong association signal at locus of CPU1 (component phosphorus uptake 1), from genome-wide study efficiency soybean core collection grown the field. A SEC12-like gene, GmPHF1, identified as causal gene CPU1. GmPHF1 facilitates ER (endoplasmic reticulum)...
Shoot branching is fundamentally important in determining soybean yield. Here, through genome-wide association study, we identify one predominant locus on chromosome 18 that confers branch number the natural population. Further analyses determine Dt2 corresponding gene and variations result significant differential transcriptional levels between two major haplotypes. Functional characterization reveals interacts with GmAgl22 GmSoc1a to physically bind promoters of GmAp1a GmAp1d activate...
Phosphorus (P) is essential for plant growth and development. Phosphate (Pi) transporter genes in the Pht1 family play important roles Pi uptake translocation plants. Although have been well studied model plants, little known about their functions soybean, an legume crop worldwide.We identified isolated a complete set of 14 (GmPT1-14) soybean genome categorized them into two subfamilies based on phylogenetic analysis. Then, experiment to elucidate transport activity GmPTs was carried out...
Flowering time and stem growth habit determine inflorescence architecture in soybean, which turn influences seed yield. Dt1, a homolog of Arabidopsis TERMINAL FLOWER 1 (TFL1), is major controller habit, but its underlying molecular mechanisms remain unclear. Here, we demonstrate that Dt1 affects node number plant height, as well flowering time, soybean under long-day conditions. The bZIP transcription factor FDc1 physically interacts with the FDc1-Dt1 complex directly represses expression...
Summary Photoperiod‐mediated flowering determines the phenological adaptability of crops including soybean ( Glycine max ). A genome‐wide association study (GWAS) identified a new time locus, Time 13 Tof13 ), which defined gene encoding an AP2/ERF transcription factor. This factor, we named TOE4b, is localized in nucleus. TOE4b has been selected for latitude adaptability. The existing natural variant H4 was rare wild accessions but occurred more frequently landraces and cultivars. Notably,...
Abstract Background High-affinity nitrate transporter 2 ( NRT2 ) genes have been implicated in absorption and remobilization under nitrogen (N) starvation stress many plant species, yet little is known about this gene family respond to various stresses often occurs the production of rapeseed Brassica napus L.). Results This report details identification 17 members rapeseed, as well as, assessment their expression profiles using RNA-seq analysis qRT-PCR assays. In study, all BnNRT2. 1...
Soybean (Glycine max L.) is a typical photoperiod-sensitive crop, such that photoperiod determines its flowering time, maturity, grain yield, and phenological adaptability. During evolution, the soybean genome has undergone two duplication events, resulting in about 75% of all genes being represented by multiple copies, which associated with rampant gene redundancy. Among duplicated genes, important maturity E2 homologs, E2-Like (E2La) b (E2Lb), encode orthologs Arabidopsis GIGANTEA (GI)....
Summary Formation of root nodules is a unique hallmark the symbiotic interaction between legume host plants and rhizobia governed by complex regulatory framework that balances appropriate orchestration rhizobial infection subsequent nodule organogenesis. In contrast to prominent model species such as Medicago truncatula Lotus japonicus , research on signal transduction in staple‐crop soybean Glycine max remains relatively insufficient. Here, we identified mutant with ~25% additional over...
Flowering time is an important agronomic trait for soybean yield and adaptation. However, the genetic basis of adaptation to diverse latitudes still not clear. Four NIGHT LIGHT-INDUCIBLE AND CLOCK-REGULATED 2 (LNK2) homeologs Arabidopsis thaliana LNK2 were identified in soybean. Three single-guide RNAs designed editing four genes. A transgene-free homozygous quadruple mutant genes was developed using CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated...
Ammonium transporters (AMTs) are plasma membrane proteins mediating ammonium uptake and transport. As such, AMTs play vital roles in acquisition mobilization, plant growth development, stress pathogen defense responses. Identification of favorable AMT genotypes is a prime target for crop improvement. However, to date, systematic identification expression analysis gene family members has not yet been reported rapeseed (Brassica napus L.). In this study, 20 genes were identified comprehensive...
Abstract With the implementation of plastic restrictions and increasingly serious problem environmental pollution, paper with green recyclable characteristics is gradually becoming a substitute for packaging materials. However, rich cellulose structure in paper, which contains hydroxyl groups leading to its hydrophilicity, allows water molecules penetrate disrupt hydrogen bonds between cellulose, resulting decrease mechanical properties. Therefore, waterproof coatings are crucial paper‐based...
Intricate cerebral cortex formation is orchestrated by the precise behavior and division dynamics of radial glial cells (RGCs). Endocytosis functions in recycling remodeling adherens junctions (AJs) response to changes RGC activity function. Here, we show that conditional disruption ubiquitin-associated protein 1 (UBAP1), a component endosomal sorting complex required for transport (ESCRT), causes severe brain dysplasia prenatal ventriculomegaly. UBAP1 depletion disrupts AJs polarity RGCs,...