A5080351529- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Glioma Diagnosis and Treatment
- Intracranial Aneurysms: Treatment and Complications
- Meningioma and schwannoma management
- Pituitary Gland Disorders and Treatments
- Immunotherapy and Immune Responses
- Vascular Malformations Diagnosis and Treatment
- Cerebrospinal fluid and hydrocephalus
- Traumatic Brain Injury and Neurovascular Disturbances
- Neurosurgical Procedures and Complications
- Head and Neck Surgical Oncology
- Spinal Fractures and Fixation Techniques
- Brain Metastases and Treatment
- CRISPR and Genetic Engineering
- T-cell and B-cell Immunology
- Cerebrovascular and Carotid Artery Diseases
- Intraoperative Neuromonitoring and Anesthetic Effects
- Spine and Intervertebral Disc Pathology
- Acute Ischemic Stroke Management
- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Cervical and Thoracic Myelopathy
- Adrenal and Paraganglionic Tumors
- Nerve injury and regeneration
Nara Medical University
2015-2024
Hiroshima University
2009-2024
Nara Medical University Hospital
2022-2023
National Archives and Records Administration
2021
Neurological Surgery
2004-2020
Stony Brook University
2020
Kanazawa University
2020
Tokyo Metropolitan Komagome Hospital
2011
Hyogo Medical University
2009
University of Pittsburgh
2004-2007
Abstract Background Toll-like receptor (TLR)3 ligands serve as natural inducers of pro-inflammatory cytokines capable promoting Type-1 adaptive immunity, and TLR3 is abundantly expressed by cells within the central nervous system (CNS). To improve efficacy vaccine strategies directed against CNS tumors, we evaluated whether administration a ligand, polyinosinic-polycytidylic (poly-IC) stabilized with poly-lysine carboxymethylcellulose (poly-ICLC) would enhance anti-CNS tumor effectiveness...
Interleukin (IL)-23 is an essential cytokine involved in expansion of the Th17 lineage, which associated with many immune-related destructive tissue diseases. We hypothesized that IL-23-induced pathway plays a role periodontal pathology and examined expression cytokines, related molecules, lesions control sites. IL-23 IL-12 were expressed at significantly higher levels than However, relative receptor compared β2 was lesions. Moreover, IL-17 lesions, especially adjacent to bone destruction,...
Background and Purpose— The primary results of the Japan Adult Moyamoya Trial revealed statistically marginal superiority bypass surgery over medical treatment alone in preventing rebleeding moyamoya disease. purpose this analysis is to test prespecified subgroup hypothesis that natural course surgical effects vary depending on hemorrhagic site at onset. Methods— site, classified as either anterior or posterior, was only stratifying variable for randomization. Statistical analyses were...
Natural killer (NK) cells are considered potential antitumor effector cells. The aim of this study was to establish a novel type chimeric antigen receptor (CAR) NK cell line (CAR-KHYG-1) specific for epidermal growth factor variant III (EGFRvIII)-expressing tumors and investigate the anti-tumor activity EGFRvIII-specific-CAR-KHYG-1 (EvCAR-KHYG-1).EvCAR-KHYG-1 established by self-inactivated lentiviral-based transduction EvCAR gene magnetic bead-based purification EvCAR-expressing effects...
Glioblastoma (GBM), which is the most common malignant brain tumor, resistant to standard treatments. Immunotherapy might be a promising alternative for treatment of this cancer. Chimeric antigen receptor (CAR) an artificially modified fusion protein that can engineered direct specificity and function T cells against tumor antigens. However, antitumor effects EGFRvIII-targeting CAR-T (EvCAR-T) in GBM are limited. The inhibitory effect induced by interaction between programmed cell death 1...
Glioblastoma (GBM) is the most common and aggressive primary malignant brain tumor in adults. Natural Killer (NK) cells are potent cytotoxic effector against inducing GBM cells; therefore, NK cell based- immunotherapy might be a promising target GBM. T immunoglobulin mucin family member 3 (TIM3), receptor expressed on cells, has been suggested as marker of dysfunctional cells. We established TIM3 knockout using clustered regularly interspaced short palindromic repeats...
Abstract Background and Aims. The purpose of the present study was to examine efficacy transplantation mouse embryonic-stem-(ES)-cell-derived tyrosine hydroxylase-positive (TH+) cells into Parkinsonian mice using behavioral tests immunohistochemical evaluation. Methods. Undifferentiated ES carrying enhanced green fluorescent protein (EGFP) gene were differentiated a cell population containing TH+ neurons five-step in vitro differentiation method. These ES-cell-derived used as allografts...
Abstract The development of effective immunotherapeutic strategies for central nervous system (CNS) tumors requires a firm understanding factors regulating the trafficking tumor antigen–specific CTLs into CNS lesions. Using C57BL/6 mice bearing intracranial (i.c.) ovalbumin-transfected melanoma (M05), we evaluated efficacy and homing i.v. transferred type 1 or 2 (Tc1 Tc2, respectively) prepared from ovalbumin-specific T-cell receptor–transgenic OT-1 mice. We also tested our hypothesis that...
The feasibility and reliability of combined use transcranial direct cortical motor evoked potential (MEP) monitoring during unruptured aneurysm surgery were evaluated. Forty-eight patients with cerebral aneurysms underwent craniotomy neck clipping accompanied by muscle MEP monitoring. MEPs elicited successfully electrical stimulation in all patients. Direct 44 Reduction amplitude to less than 50% baseline was considered significant. No postoperative paresis occurred 39 whom remained...
Abstract We have previously shown preferential tumor-homing and therapeutic efficacy of adoptively transferred type 1 CTL (Tc1) when compared with 2 (Tc2) in mice bearing intracranial ovalbumin-transfected melanoma (M05). Further characterizing the expression a panel homing receptors on Tc1 Tc2 cells, we found that very late antigen (VLA)-4 (a heterodimer CD49d CD29), but none other evaluated, was expressed at significantly higher levels cells than cells. Although (α4 integrin) can form...
Glioblastoma is the leading malignant glioma with a poor prognosis. This study aimed to investigate antitumor effects of natural killer cells in combination temozolomide as standard chemotherapeutic agent for glioblastoma. Using simple, feeder-less, and chemically defined culture method, we expanded human peripheral blood mononuclear assessed receptor expression, cell activity, regulatory T frequency cells. Next, using glioblastoma lines (temozolomide-sensitive U87MG, temozolomide-resistant...
To evaluate cervical facet joint degeneration using a newly developed classification, investigate its prevalence and relationship with degenerative spondylolisthesis, clarify clinical significance in patients myelopathy (DCM).This study included 145 consecutive DCM who underwent surgical treatment. Clinical variables radiological findings were analyzed. A new 6-grade computed tomography (CT) classification for was adapted, evaluated by categorizing the joints into those at responsible...
BACKGROUND: A novel tyrosine kinase receptor EphA2 is expressed at high levels in advanced and metastatic cancers. We examined whether vaccinations with synthetic mouse (mEphA2)-derived peptides that serve as T cell epitopes could induce protective therapeutic anti-tumor immunity. METHODS: C57BL/6 mice received subcutaneous (s.c.) bone marrow-derived dendritic cells (DCs) pulsed recognized by CD8+ (mEphA2671-679, mEphA2682-689) CD4+ (mEphA230-44) cells. Splenocytes (SPCs) were harvested from...
Abstract Restricted and high-level expression of interleukin-13 receptor α2 (IL-13Rα2) in a majority human malignant gliomas makes this protein an attractive vaccine target. We have previously described the identification IL-13Rα2345-353 peptide as leukocyte antigen-A2 (HLA-A2)–restricted CTL epitope. However, it remains unclear how efficiently peptide-based vaccines can induce specific CTLs patients with gliomas, we examined whether analogue epitopes could elicit heteroclitic antitumor...
Embryonic stem (ES) cells are a potential source for treatment of spinal cord injury (SCI). Although one the main problems ES cell-based cell therapy is tumor formation, there no ideal method to suppress development. In this study, we examined whether transplantation with bone marrow stromal (BMSCs) prevented formation in SCI model mice that received cell-derived grafts containing both undifferentiated and neural cells. Embryoid bodies (EBs) formed 4-day hanging drop cultures were treated...
Cell-based immunotherapy is categorized as a regenerative therapy under the Regenerative Medicine Safety Act in Japan. Natural killer (NK) cell-based considered promising strategy for treating cancer, including glioblastoma (GBM). We previously reported an expansion method highly purified human peripheral blood-derived NK cells using cytokine cocktail. Here, we aimed to establish more efficient cell compared our method. T cell-depleted blood mononuclear (PBMCs) were isolated from three...
Abstract We tested whether modulation of the CNS-tumor microenvironment by delivery IFN-α-transduced dendritic cells (DCs: DC-IFN-α) would enhance therapeutic efficacy peripheral vaccinations with cytokine-gene transduced tumor cells. Mice bearing intracranial GL261 glioma or MCA205 sarcoma received immunizations corresponding irradiated engineered to express IL-4 GM-CSFs, respectively, as well intratumoral DC-IFN-α. This regimen prolonged survival animals and induced tumor-specific CTLs...
<b><i>Background:</i></b> Hyponatremia is a common complication occurring in one third of patients after subarachnoid hemorrhage (SAH). One mechanism that likely mediates the development hyponatremia SAH cerebral salt wasting syndrome (CSWS), which induces natriuresis and reduces total blood volume, resulting risk symptomatic vasospasm (SVS). The mineral corticoid fludrocortisone acetate enhances sodium reabsorption renal distal tubules may help prevent post-SAH...
Aims: To investigate the efficacy of embryonic stem cell-derived neural cells (NSCs) for spinal cord injury (SCI) in mice and whether a combination treatment with thyroid hormone provides more effective ES cell-based therapy. Methods: Nestin-positive NSCs were induced from undifferentiated mouse by step-by-step culture used as grafts. Thirty-six subjected to an SCI at Th10 divided into three groups 12. Graft transplanted site 10 days after injury. Group 1 left under observation without...