A5013683592- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Dysphagia Assessment and Management
- Child Nutrition and Feeding Issues
- Cancer Immunotherapy and Biomarkers
- Hepatocellular Carcinoma Treatment and Prognosis
- PI3K/AKT/mTOR signaling in cancer
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Cancer-related gene regulation
- Ear Surgery and Otitis Media
- RNA regulation and disease
- Head and Neck Cancer Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Esophageal Cancer Research and Treatment
- Cancer-related molecular mechanisms research
- Pancreatic and Hepatic Oncology Research
- Vector-Borne Animal Diseases
- Polyamine Metabolism and Applications
- Genomics and Chromatin Dynamics
- Gastrointestinal Tumor Research and Treatment
- Antibiotic Use and Resistance
- Phytochemical compounds biological activities
Systems Biology Institute
2010-2024
RIKEN Center for Biosystems Dynamics Research
2018-2024
University of Yamanashi
2019-2024
Tokai University
2020-2023
National Cancer Center Hospital East
2004-2022
Showa University
2012-2022
RIKEN Center for Integrative Medical Sciences
2021-2022
Tokushima University Hospital
2022
Juntendo University
2022
The University of Tokyo
2009-2020
The recurrence rates of Hepatocellular carcinoma (HCC) are high, necessitating novel and effective adjuvant therapies. Therefore, we conducted a phase II study glypican-3 (GPC3) peptide vaccine as an therapy for HCC patients. Forty-one patients with initial who had undergone surgery or radiofrequency ablation (RFA) were analyzed in this II, open-label, single-arm trial. Ten vaccinations performed 1 y after curative treatment. We also investigated case-control subjects, where selected treated...
Integrated stress response on the brain During translation, regulation of protein synthesis by phosphorylation eukaryotic translation initiation factor 2 (eIF2) is a common consequence diverse stimuli, which leads to reprogramming gene expression. This process, known as integrated response, one most fundamental mechanisms translational control conserved throughout eukaryotes. It also promising therapeutic target in neurodegenerative diseases and traumatic injury. Kashiwagi et al. report...
The use of allogeneic, pluripotent stem-cell-derived immune cells for cancer immunotherapy has been the subject recent clinical trials. In Japan, investigator-initiated trials will soon begin ovarian treatment using human leukocyte antigen (HLA)-homozygous-induced stem cell (iPSC)-derived anti-glypican-3 (GPC3) chimeric receptor (CAR)-expressing natural killer/innate lymphoid (NK/ILC). Using as source allogeneic facilitates stringent quality control final product, in terms efficacy, safety...
While multiple squamous cell carcinomas are often observed in the esophagus and head neck region confound us about favorable treatments, reason why some patients more likely to develop cancers remains obscure. We statistically analyzed clinical factors 203 with newly diagnosed carcinoma, assess risk of for establishment an effective prevention screening programs. Widespread epithelial oncogenic alterations were assessed as lugol-voiding lesions (multiple LVL) using lugol chromoendoscopy....
Specific identification of bacteria has been achieved through precisely transferred bacterial structure on the surface overoxidized polypyrrole (OPPy) film. The recognition target was successfully carried out in real time using OPPy film combination with dielectrophoresis. unique both techniques made specific detection template bacilli possible at concentrations as low 10(3) CFU/mL within 3 min, without any pretreatment. observation movement by a fluorescent microscope revealed that living...
Glypican-3 (GPC3), a carcinoembryonic antigen, is an ideal target for anticancer immunotherapy against hepatocellular carcinoma (HCC). In this study, we attempted to compare the induction of GPC3-specific T-cell-mediated immune response after locoregional therapies in HCC patients and tumor-bearing mice. Twenty-seven treated with therapies, including radiofrequency ablation (RFA), surgical resection transcatheter arterial chemoembolization (TACE), were prospectively enrolled study....
The CUG-binding protein 1 (CUG-BP1) is a member of the CUG-BP1 and ETR-like factors (CELF) family or Bruno-like involved in control splicing, translation mRNA degradation. Several target RNA sequences have been predicted, such as CUG triplet repeat, GU-rich AU-rich element nuclear pre-mRNAs and/or cytoplasmic mRNA. has three RNA-recognition motifs (RRMs), among which third RRM (RRM3) can bind to RNAs on its own. In this study, we solved solution structure RRM3 by hetero-nuclear NMR...
Human Transformer2-β (hTra2-β) is an important member of the serine/arginine-rich protein family, and contains one RNA recognition motif (RRM). It controls alternative splicing several pre-mRNAs, including those calcitonin/calcitonin gene-related peptide (CGRP), survival motor neuron 1 (SMN1) tau protein. Accordingly, RRM hTra2-β specifically binds to two types sequences [the CAA (GAA)2 sequences]. We determined solution structure (spanning residues Asn110–Thr201), which not only has a...
Abstract Various stressors such as viral infection lead to the suppression of cap-dependent translation and activation integrated stress response (ISR), since stress-induced phosphorylated eukaryotic initiation factor 2 [eIF2(αP)] tightly binds eIF2B prevent it from exchanging guanine nucleotide molecules on its substrate, unphosphorylated eIF2. Sandfly fever Sicilian virus (SFSV) evades this through interaction between nonstructural protein NSs host eIF2B. However, precise mechanism has...
Protein methylation occurs predominantly on lysine and arginine residues, but histidine also serves as a substrate. However, limited number of enzymes responsible for this modification have been reported. Moreover, the biological role has remained poorly understood to date. Here, we report that human METTL18 is methyltransferase ribosomal protein RPL3 specifically slows ribosome traversal Tyr codons, allowing proper folding synthesized proteins. By performing an in vitro assay with methyl...
The WWE domain is often identified in proteins associated with ubiquitination or poly‐ADP‐ribosylation. Structural information about domains has been obtained for the ubiquitination‐related proteins, such as Deltex and RNF146, but not yet poly‐ADP‐ribose polymerases (PARPs). Here we determined solution structures of from PARP11 PARP14, compared them that RNF146 domain. NMR perturbation experiments revealed specific differences their ADP‐ribose recognition modes correlated individual...
Antigen-specific cancer immunotherapy is a promising strategy for improving treatment. Recently, many tumor-associated antigens and their epitopes recognized by cytotoxic T lymphocytes (CTLs) have been identified. However, the density of endogenously presented antigen-derived peptides on tumor cells generally sparse, resulting in inability antigen-specific CTLs to work effectively. We hypothesize that increasing an peptide would enhance immunotherapy. Here, we demonstrated intratumoral...
Abstract We developed fluorescent turn‐on probes containing a nucleoside, 5‐(benzofuran‐2‐yl)deoxyuridine (dU BF ) or 5‐(3‐methylbenzofuran‐2‐yl)deoxyuridine MBF ), for the detection of single‐stranded DNA RNA by utilizing triplex formation. Fluorescence measurements revealed that probe dU achieved superior fluorescence enhancement than . NMR and analyses indicated intensity increased upon formation partly as consequence conformational change at bond between 3‐methylbenzofuran uracil rings....
A polarization-sensitive Fourier domain optical coherence tomography (PS-FD-OCT) system is demonstrated. This OCT based on a spectral interferometer, does not require mechanical axial scanning, and enables phase information to be used in an image. Owing this information, the requires only two measurements for determining Jones matrix images Müller of biological samples. reveals birefringence properties inner surface porcine esophagus.
We recently reported the safety, immunological and clinical responses to a GPC3-derived peptide vaccine in phase I trial of patients with advanced hepatocellular carcinoma (HCC). conducted subsequent HCC assess histopathological findings before after vaccination GPC3 peptide. Here, we present course pathological study including autopsy patient ongoing trial. A 62-year old suffering from refractory sorafenib therapy received vaccine. The had fever remarkably impaired liver function twice...
Glypican-3 (GPC3) is a glycosylphosphatidylinositol-anchored cell surface protein overexpressed in hepatocellular carcinoma(HCC), and its overexpression associated with poor prognosis. The diagnostic potential of GPC3 as serum marker has been reported. In the present study, we evaluated usefulness plasma predictor for recurrence after surgical resection stage I HCC patients by newly developed an enzyme-linked immunosorbent assay (ELISA) system. Current study demonstrated that high levels...
Abstract The MYO7A gene is known to be responsible for both syndromic hearing loss (Usher syndrome type1B:USH1B) and non-syndromic including autosomal dominant recessive inheritance (DFNA11, DFNB2). However, the prevalence detailed clinical features of -associated across a large population remain unclear. In this study, we conducted next-generation sequencing analysis cohort 10,042 Japanese patients. As result, 137 patients were identified with so that among was 1.36%. We 70 disease-causing...