A5004162530- Cellular Mechanics and Interactions
- Innovations in Medical Education
- Anatomy and Medical Technology
- Force Microscopy Techniques and Applications
- Surgical Simulation and Training
- 3D Printing in Biomedical Research
- Homelessness and Social Issues
- Particle physics theoretical and experimental studies
- Appendicitis Diagnosis and Management
- Tendon Structure and Treatment
- Diversity and Career in Medicine
- Hernia repair and management
- Cardiac, Anesthesia and Surgical Outcomes
- Social Media in Health Education
- Polymer Surface Interaction Studies
- Marine Biology and Environmental Chemistry
- Diverticular Disease and Complications
- Heat shock proteins research
- Lipid Membrane Structure and Behavior
- Teacher Education and Leadership Studies
- Dental Research and COVID-19
- Clinical Reasoning and Diagnostic Skills
- Bariatric Surgery and Outcomes
- Marine Invertebrate Physiology and Ecology
- Metal-Catalyzed Oxygenation Mechanisms
George Washington University
2014-2024
Westfield State University
2024
GW Medical Faculty Associates
2024
George Washington University Hospital
2024
American College of Surgeons
2023-2024
Barnard College
2021-2023
American College of Financial Services
2023
Pacific Institute For Research and Evaluation
2016-2020
University of Pittsburgh
2020
University of Connecticut
2001-2018
Fish keratocytes can generate rearward directed traction forces within front portions of the lamellipodium, suggesting that a retrograde flow actin may also occur here but this was not detected by previous photoactivation experiments. To investigate relationship between and force generation, we have transfected with GFP-actin used fluorescent speckle microscopy, to observe flow. We leading lamellipodium is inversely proportional both protrusion rate cell speed. effect reducing contractility,...
Abstract Alkanes are the most energy-rich form of carbon and widely dispersed in environment. Their transformation by microbes represents a key step global cycle. Alkane monooxygenase (AlkB), membrane-spanning metalloenzyme, converts straight chain alkanes to alcohols first microbially-mediated degradation alkanes, thereby playing critical role cycling bioremediation oil. AlkB biodiversity is attributed its ability oxidize various lengths, while individual AlkBs target relatively narrow...
ABSTRACT Close contacts are the predominant type of cell-substratum adhesion in rapidly moving cells yet little is known about their composition and dynamics. To address these issues we have attempted to identify molecular components close formed fish epithelial keratocytes. In addition utilized simple shape keratocytes explore relationship between contact formation rapid locomotion. β1 Integrin talin molecules were found be localized within a narrow rim very along leading edge. These...
The objective of this study was to determine the direction membrane lipid flow in locomoting cells. plasma human polymorphonuclear leukocytes stained with a fluorescent analog dihexadecanoyl indocarbocyanine. A line photobleached on cell surface perpendicular motion. Low-light-level fluorescence microscopy and digital image-processing techniques were used analyze series images taken at short intervals after photobleaching. bleached remained visible for about 5 seconds before being erased by...
This paper examines neighborhood, outlet, and server characteristics related to successful purchases of alcohol by intoxicated patrons underage drinkers at establishments. It is hypothesized that outlets in commercial areas near other premises, with poor exterior maintenance, much advertising, inexperienced youthful servers will be more likely sell patrons.Data were collected using pseudo-intoxicated patron apparent minor surveys randomly selected establishments a metropolitan area northern...
Continuous cell movement requires the coordination of protrusive forces at leading edge with contractile rear cell. Myosin II is required to generate necessary force facilitate retraction; however, Dictyostelium cells that lack myosin (mhcA-) are still motile. To directly investigate role in contractility we used a gelatin traction assay measure magnitude and dynamic redistribution stresses generated by randomly moving wild-type, essential light chain null (mlcE-) mhcA- cells. Our data show...
Human heat shock 27-kDa protein 1 (HSPB1)/heat (Hsp) 27 is a small which thought to have several roles within the cell. One of these includes regulating actin filament dynamics in cell movement, since Hsp27 has previously been found inhibit polymerization vitro. In this study, role further investigated. levels were reduced using siRNA SW480 cells, human colon cancer line. An vitro wound closure assay showed that cells with knocked down unable close wounds, indicating involved motility....
American Indian and Alaska Native (AI/AN) youth have the highest commercial tobacco smoking rate of any group in United States. Unified by aim to reduce AI/AN use, six separately funded prevention programs serving Southern California tribes formed Tribal Tobacco Coalition (SCTTC). Since joining forces, SCTTC has hosted various activities events that encourage community involvement, leadership, commitment tradition. The SCTTC’s pinnacle event, annual Inter-Tribal Prevention Youth Summit, is...
The coordination of protrusion with retraction is essential for continuous cell movement. In fish keratocytes the activation stretch-activated calcium channels, and resulting increase in intracellular calcium, trigger release rear margin when forward movement impeded. Although it likely that involves a calcium-dependent cytoskeletal contractility, not known how timing, magnitude localization contractile forces are organized during retraction. We have addressed this question using new gelatin...
Surgical anatomy is taught early in medical school training. The literature shows that many physicians, especially surgical specialists, think anatomical knowledge of students inadequate and nesting sciences later the clinical curriculum may be necessary. Quantitative data concerning this perception an deficit are lacking, as specifics to what content should reinforced. This study identifies baseline areas strength weakness entering rotations. Third‐year completed a 20–25‐question test at...
In order to find the helicity of neutrino nu u associated with mu meson, reaction pi --- -+ /sub / is studied. The found from asymmetry in Mott scattering polarized - mesons produced reaction. It that helicities and e/ are equal. (T.F.H.)
Background: Residency program applicants use a variety of resources during the application cycle. Program websites can vary substantially, and it is unclear how website information used by applicants. We aimed to determine most popular source also sought identify specific online content that was deemed important in decision-making process. Methods: A survey distributed fourth-year medical students at an academic institution. Demographic collected, importance various gauged using Likert...
The activation of stretch-activated calcium channels (SACs) in keratocytes can induce spatially coordinated increases traction stress that promote protrusion at the cell front, while simultaneously inducing retraction rear. To investigate how this occurs, we correlated calcium-induced changes with alterations speed and shape. Cyclic these parameters were associated each transient. In addition, an inverse relationship was found between speed, suggesting alternating adhesiveness occurring We...
The search for the ic 1100 deg C are e + decay mode was made by observing coincidences between a gamma detector and an electron placed on either side of source decaying positive muons. These were obtained stopping 60-Mev effects /sup +/ beam Nevis cyclotron in lithium target. Theres mesons decayed into muons whose range too short them to escape from A branching ratio less than 2 x 10/sup -6/ found with confidence level 90%. (W.D.M.)
Release of GnRH surges in female rats is directed by a daily neural signal and occurs only after exposure the hypothalamus to sustained, elevated estrogen (E2) levels serum. We have proposed that preovulatory E2 couples circuitry governing release two-step process, which includes stimulation neuronal progesterone receptors (PRs) subsequent activation PRs signal. In preceding report we documented PR obligatory for E2. these studies assess validity second essential feature this model, signals...
Complicated skin and structure infections (cSSSIs) frequently result in hospitalization with significant morbidity mortality. In this phase 3b/4 parallel, randomized, open-label, comparative study, 531 subjects cSSSI received tigecycline (100 mg initial dose, then 50 intravenously every 12 hrs) or ampicillin-sulbactam 1.5-3 g IV 6 hrs amoxicillin-clavulanate 1.2 6-8 hrs. Vancomycin could be added at the discretion of investigator to comparator arm if methicillin-resistant Staphylococcus...
The diagnosis of acute appendicitis can be surprisingly difficult without computed tomography, which carries significant radiation exposure. Circulating blood cells may carry informative changes in their RNA expression profile that would signal internal infection or inflammation the appendix. Genome-wide profiling was applied to whole patients versus with other abdominal disorders, order identify biomarkers appendicitis. From a large cohort emergency patients, discovery set surgically...