A5024904221- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Angiogenesis and VEGF in Cancer
- Acute Lymphoblastic Leukemia research
- Renal and related cancers
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Acute Myeloid Leukemia Research
- Lymphoma Diagnosis and Treatment
- Cytomegalovirus and herpesvirus research
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Chronic Lymphocytic Leukemia Research
- Polyomavirus and related diseases
- Virus-based gene therapy research
- Renal Transplantation Outcomes and Treatments
- Immunotherapy and Immune Responses
- Chronic Myeloid Leukemia Treatments
- Immunodeficiency and Autoimmune Disorders
- Immune cells in cancer
- Neonatal Respiratory Health Research
- Transplantation: Methods and Outcomes
- Prenatal Screening and Diagnostics
- CRISPR and Genetic Engineering
- T-cell and Retrovirus Studies
Hyogo University
2023
Hyogo Medical University
2004-2019
Reha Rheinfelden
2016
Yamagata Research Institute Of Technology
2016
Osaka Minami Medical Center
2002-2009
KU Leuven
2008-2009
Kyoto University
2009
Catholic University of America
2008
Osaka University
1996-2007
National Hospital Organization
2006
Thrombo-occlusive cerebrovascular disease resulting in stroke and permanent neuronal loss is an important cause of morbidity mortality. Because the unique properties cerebral vasculature limited reparative capability tissue, it has been difficult to devise effective neuroprotective therapies ischemia. Our results demonstrate that systemic administration human cord blood–derived CD34+ cells immunocompromised mice subjected 48 hours earlier induces neovascularization ischemic zone provides a...
Thrombo-occlusive cerebrovascular disease resulting in stroke and permanent neuronal loss is an important cause of morbidity mortality. Because the unique properties cerebral vasculature limited reparative capability tissue, it has been difficult to devise effective neuroprotective therapies ischemia. Our results demonstrate that systemic administration human cord blood–derived CD34+ cells immunocompromised mice subjected 48 hours earlier induces neovascularization ischemic zone provides a...
To determine the role of Wilms' tumor gene WT1 in tumorigenesis solid tumors, expression was examined 34 cell lines (four gastric cancer lines, five colon 15 lung four breast one germ line, two ovarian uterine thyroid and hepatocellular carcinoma line) by means quantitative reverse transcriptase-polymerase chain reaction. detected three all 12 line. Therefore, examined, 28 (82%) expressed WT1. Three expressing (gastric line AZ-521, OS3, TYK-nu) were further analyzed for mutations and/or...
Increasing evidence points to a role for circulating endothelial progenitor cells, including populations of CD34- and CD133-positive cells present in peripheral blood, maintenance the vasculature neovascularization. Immature populations, CD34-positive have been shown contribute vascular homeostasis, not only as pool but also source growth/angiogenesis factors at ischemic loci. We hypothesized that diminished numbers immature might impair such physiological reparative processes, potentially...
The short-term clinical benefits of bone marrow mononuclear cell transplantation have been shown in patients with critical limb ischemia. purpose this study was to assess the long-term safety and efficacy thromboangiitis obliterans.Eleven limbs (3 rest pain 8 an ischemic ulcer) were treated by transplantation. followed up for events a mean 684+/-549 days (range 103 1466 days). At 4 weeks, improvement observed all 11 limbs, complete relief (36%). Pain scale (visual analog scale) score...
Abstract Increasing evidence shows that administration of bone marrow mononuclear cells (BMMCs) is a potential treatment for various ischemic diseases, such as stroke. Although angiogenesis has been considered primarily responsible the effect BMMCs, their direct contribution to endothelial (ECs) by being functional elements vascular niches neural stem/progenitor (NSPCs) not considered. Herein, we examine whether BMMCs affected properties ECs and NSPCs, they promoted neurogenesis recovery...
The goal of this clinical trial was to assess the feasibility and safety transplanting autologous bone marrow mononuclear cells into patients suffering severe embolic stroke. Major inclusion criteria included with cerebral embolism, age 20–75 years, National Institute Health Stroke Scale (NIHSS) score displaying improvement ≤5 points during first 7 days after stroke, NIHSS ≥10 on day Bone aspiration (25 or 50 mL; N = 6 in each case) performed 7–10 poststroke, were administrated...
Although mesenchymal stem cells (MSCs) can be obtained from the fetal membrane (FM), little information is available regarding biological differences in MSCs derived different layers of FM or their therapeutic potential. Isolated both amnion and chorion showed similar morphological appearance, multipotency, cell-surface antigen expression. Conditioned media amnion- chorion-derived inhibited cell death caused by serum starvation hypoxia endothelial cardiomyocytes. Amnion secreted significant...
The CNS has the potential to marshal strong reparative mechanisms, including activation of endogenous neurogenesis, after a brain injury such as stroke. However, response neural stem/progenitor cells stroke is poorly understood. Recently, have been identified in cerebral cortex, well previously recognized regions subventricular or subgranular zones hippocampus, suggesting that contribution cortex-derived may repair ischemic lesions cortex. In present study, using highly reproducible murine...
Abstract Acute inflammation in the poststroke period exacerbates neuronal damage and stimulates reparative mechanisms, including neurogenesis. However, only a small fraction of neural stem/progenitor cells survives. In this report, by using highly reproducible model cortical infarction SCID mice, we examined effects immunodeficiency on reduction brain injury, survival cells, functional recovery. Subsequently, contribution T lymphocytes to neurogenesis was evaluated mice depleted for each...
Summary. The Wilms' tumour gene, WT1, is expressed at high levels in leukaemia cells and plays an important role leukaemogenesis. WT1 also human normal CD34 + bone marrow (BM) about 100 times lower than cells. To identify characterize WT1‐expressing BM cells, they were sorted into single analysed for expression using two kinds of single‐cell reverse transcriptase polymerase chain reaction (RT–PCR) methods. Using the semiquantitative polyA‐PCR sequence‐specific (SS)‐PCR method, was detected...
Increasing evidence points to a role for circulating endothelial progenitor cells, including populations of CD34-positive (CD34 + ) in maintenance cerebral blood flow. In this study, we investigated the link between level CD34 cells and neovascularization at ischemic brain. Compared with control subjects, remarkable increase was observed patients angiographic moyamoya vessels, although no significant change major artery occlusion (or severe stenosis) but without vessels. Our results suggest...
Abstract The administration of CD34‐positive cells after stroke has been shown to have a beneficial effect on functional recovery by accelerating angiogenesis and neurogenesis in rodent models. Granulocyte colony‐stimulating factor (G‐CSF) is known mobilize from bone marrow displayed neuroprotective properties transient ischemic stress. This led us investigate the effects G‐CSF mouse. We utilized permanent ligation M1 distal portion left middle cerebral artery develop reproducible focal...
Abstract Aims Circulating progenitor cells such as CD34+ play a key role in maintenance of vascular endothelial function and neovascularization, decrease the number is associated with cardiovascular disease. However, contribution circulating to microvascular disease, diabetic nephropathy, unclear. This study was therefore designed clarify association between nephropathy cells. Methods We measured cell numbers 85 Type 2 patients aged 40–70 years normo‐ microalbuminuria determined urinary...
Abstract According to previous reports, the frequency of Epstein–Barr virus (EBV) positivity in diffuse large B‐cell lymphoma is higher East Asia (approximately 9%) than Western countries. The presence EBV genome was examined patients registered with Osaka Lymphoma Study Group (OLSG) Osaka, Japan, situated Asia. EBV‐positive rate situ hybridization (ISH) 484 immunocompetent OLSG. male‐to‐female ratio 1.29, ages ranging from 16 95 (median, 68) years. ISH EBV‐encoded small RNAs (EBER) probes...